{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T19:51:49Z","timestamp":1772913109765,"version":"3.50.1"},"reference-count":31,"publisher":"Journal of Neurosurgery Publishing Group (JNSPG)","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2001,1]]},"abstract":"<jats:p content-type=\"fine-print\"><jats:italic>Object.<\/jats:italic> The <jats:italic>neurofibromatosis Type 2 (NF2)<\/jats:italic> gene is the only tumor suppressor gene that has been clearly implicated in the development of benign meningiomas. Interestingly, previous data obtained by the authors indicate that reduced NF2 protein expression seldom occurs in meningothelial meningiomas, the most common histological type of meningioma. The goal of the current study was to explore further the hypothesis of <jats:italic>NF2<\/jats:italic> gene-independent tumorigenesis of meningothelial meningiomas.<\/jats:p>\n<jats:p content-type=\"fine-print\"><jats:italic>Methods.<\/jats:italic> The authors performed a mutational analysis of all 17 exons of the <jats:italic>NF2<\/jats:italic> gene by using single-stranded conformational polymorphism (SSCP). In addition, expression levels of the NF2 protein and (\u00b5-calpain, a protease suggested to inactivate the NF2 protein, were determined by immunoblotting analysis of 27 meningiomas (20 meningothelial and seven nonmeningothelial). Mutations of the <jats:italic>NF2<\/jats:italic> gene were found in only one (5%) of 20 meningothelial meningiomas and three (43%) of seven nonmeningothelial tumors (Fisher's exact test, p = 0.042). The levels of NF2 protein were severely reduced in six (28.5%) of 21 meningothelial meningiomas, in contrast to six (86%) of seven nonmeningothelial meningiomas (Fisher's exact test, p = 0.023). Activation of (\u00b5-calpain did not correlate with the status of NF2 protein expression in the meningiomas analyzed, demonstrating that (\u00b5-calpain activation does not account for the loss of NF2 protein in meningiomas with apparently normal <jats:italic>NF2<\/jats:italic> genes.<\/jats:p>\n<jats:p content-type=\"fine-print\"><jats:italic>Conclusions.<\/jats:italic> These results clearly demonstrate that <jats:italic>NF2<\/jats:italic> gene mutations and decreased NF2 protein expression rarely occur in meningothelial meningiomas compared with other histological types of meningiomas. The clinical behavior of meningothelial meningiomas, however, is similar to that of other benign meningiomas. It is likely, therefore, that the tumorigenesis of meningothelial meningiomas is the result of deleterious alterations of genes that have final phenotypical effects similar to inactivation of the <jats:italic>NF2<\/jats:italic> gene.<\/jats:p>","DOI":"10.3171\/jns.2001.94.1.0111","type":"journal-article","created":{"date-parts":[[2009,5,13]],"date-time":"2009-05-13T17:41:45Z","timestamp":1242236505000},"page":"111-117","source":"Crossref","is-referenced-by-count":36,"title":["Molecular alterations in the neurofibromatosis Type 2 gene and its protein rarely occurring in meningothelial meningiomas"],"prefix":"10.3171","volume":"94","author":[{"given":"James J.","family":"Evans","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sin-Soo","family":"Jeun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joung H.","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jyoti A.","family":"Harwalkar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yigal","family":"Shoshan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John K.","family":"Cowell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mladen","family":"Golubic","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1754","reference":[{"key":"b1","series-title":"Human Gene Mutation.","author":"Cooper","year":"1994"},{"key":"b2","series-title":"Anticancer Res","first-page":"845","article-title":"Cytogenetic changes in 67 cranial and spinal meningiomas: relation to histopathological and clinical pattern.","volume":"13","author":"Doco-Fenzy","year":"1993"},{"key":"b3","series-title":"Surg Neurol","doi-asserted-by":"publisher","first-page":"131","DOI":"10.1016\/0090-3019(89)90201-2","article-title":"Multiple meningiomas and their relation to neurofibromatosis. 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