{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T21:58:00Z","timestamp":1767650280919},"reference-count":39,"publisher":"Journal of Neurosurgery Publishing Group (JNSPG)","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2002,7]]},"abstract":"Object.<\/jats:italic> Gene transfer to cerebral vessels is a promising new therapeutic approach for cerebral vasospasm after subarachnoid hemorrhage (SAH). This study was undertaken to explore whether a delayed treatment with adenovirus encoding the prepro-calcitonin<\/jats:italic> gene\u2014related peptide (CGRP), 2 days after initial blood injection, reduces cerebral vasospasm in a double-hemorrhage model of severe vasospasm in dogs.<\/jats:p>\nMethods.<\/jats:italic> In 20 dogs, arterial blood was injected into the cisterna magna on Days 0 and 2. Thirty minutes after the second blood injection, the animals received either adenovirus encoding the prepro-CGRP<\/jats:italic> gene (AdCMVCGRP\u2014treated group, eight dogs) or adenovirus encoding the \u03b2-galactosidase<\/jats:italic> gene (AdCMV\u03b2gal\u2014treated group, six dogs) under the cytomegalovirus (CMV) promoter. One group of dogs did not receive treatment and served as controls (control SAH group, six dogs). Angiography was performed on Days 0 and 7 to assess cerebral vasospasm. On Day 7 following angiography, the animals were killed and their brains were stained with X-gal to detect the distribution of gene expression. Cerebrospinal fluid (CSF) was also tested for CGRP immunoreactivity.<\/jats:p>\nSevere vasospasm was observed in control SAH dogs on Day 7, and the mean basilar artery (BA) diameter was 53.4 \u00b1 5.5% of the value measured on Day 0. Treatment with AdCMV\u03b2gal did not alter vasospasm (the BA diameter was 55 \u00b1 3.9% of that measured on Day 0). The leptomeninges and adventitia of the BAs of dogs treated using AdCMV\u03b2gal demonstrated positive staining with X-gal. High levels of CGRP were measured in CSF from dogs that received AdCMVCGRP. In the group treated with AdCMVCGRP, vasospasm was significantly reduced (the BA diameter was 78.2 \u00b1 5.3% of that measured on Day 0, p < 0.05 compared with the control SAH group and the AdCMV\u03b2gal group).<\/jats:p>\nConclusions.<\/jats:italic> In a model of severe vasospasm in dogs, gene transfer of CGRP after injection of blood attenuated cerebral vasospasm after SAH.<\/jats:p>","DOI":"10.3171\/jns.2002.97.1.0136","type":"journal-article","created":{"date-parts":[[2009,5,13]],"date-time":"2009-05-13T17:47:43Z","timestamp":1242236863000},"page":"136-142","source":"Crossref","is-referenced-by-count":26,"title":["Posttreatment with adenovirus-mediated gene transfer of calcitonin gene\u2014related peptide to reverse cerebral vasospasm in 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