{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T21:45:37Z","timestamp":1783633537853,"version":"3.55.0"},"reference-count":50,"publisher":"Journal of Neurosurgery Publishing Group (JNSPG)","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2003,12]]},"abstract":"<jats:p content-type=\"fine-print\"><jats:italic>Object.<\/jats:italic> This study was conducted to evaluate the effects of grafting encapsulated basic fibroblast growth factor (bFGF)\u2014secreting cells in rat brains subjected to ischemic injury.<\/jats:p>\n<jats:p content-type=\"fine-print\"><jats:italic>Methods.<\/jats:italic> Two cell lines were used for encapsulated grafting in this experiment, namely, a bFGF-secreting cell line established by genetic manipulation of baby hamster kidney (BHK) cells, and a naive BHK cell line. Forty-seven Sprague\u2014Dawley rats were used in this experiment. The animals were divided into the following three groups: those receiving grafts of encapsulated bFGF-secreting cells (BHK-bFGF group); those with grafts of encapsulated naive BHK cells (naive BHK group); and those with no grafts (control group). The authors implanted encapsulated cells into the right striatum of host rats in the BHK-bFGF and naive BHK groups. Six days after grafting, the host and control animals underwent permanent right middle cerebral artery occlusion (MCAO) with an intraluminal suture procedure. The infarct volume was evaluated using 2,3,5-triphenyltetrazolium chloride staining and computerized image analysis 24 hours after MCAO. Fragmentations of DNA in the host brains were analyzed using terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling 12 hours after MCAO.<\/jats:p>\n<jats:p content-type=\"fine-print\">The authors found that the infarct volume in the BHK-bFGF group was reduced by approximately 30% compared with that in the naive BHK and control groups. In the ischemic penumbral area, the number of apoptotic cells in the BHK-bFGF group was significantly decreased compared with that in the other groups.<\/jats:p>\n<jats:p content-type=\"fine-print\"><jats:italic>Conclusions.<\/jats:italic> The grafting of encapsulated BHK bFGF-secreting cells protected the brain from ischemic injury. Encapsulation and grafting of genetically engineered cells such as bFGF-secreting cells is thus thought to be a useful method for protection against cerebral ischemia.<\/jats:p>","DOI":"10.3171\/jns.2003.99.6.1053","type":"journal-article","created":{"date-parts":[[2009,5,13]],"date-time":"2009-05-13T17:49:07Z","timestamp":1242236947000},"page":"1053-1062","source":"Crossref","is-referenced-by-count":24,"title":["Reduction of infarct volume and apoptosis by grafting of encapsulated basic fibroblast growth factor\u2014secreting cells in a model of middle cerebral artery occlusion in rats"],"prefix":"10.3171","volume":"99","author":[{"given":"Kenjiro","family":"Fujiwara","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isao","family":"Date","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsuro","family":"Shingo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideyuki","family":"Yoshida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuki","family":"Kobayashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akira","family":"Takeuchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akimasa","family":"Yano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Tamiya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Ohmoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1754","reference":[{"key":"b1","series-title":"Neurosci Lett","first-page":"37","article-title":"Amelioration of brain edema by topical application of glial cell line-derived neurotrophic factor in reperfused rat brain.","volume":"231","author":"Abe","year":"1997"},{"key":"b2","series-title":"Exp Neurol","first-page":"269","article-title":"Long-term cross-species brain transplantation of a polymer encapsulated dopamine-secreting cell line.","volume":"111","author":"Aeibisher","year":"1991"},{"key":"b3","series-title":"Brain Res Mol Brain Res","first-page":"71","article-title":"Intravenous basic fibroblast growth factor (bFGF) decreases DNA fragmentation and prevents downregulation of Bcl-2 expression in the ischemic brain following middle cerebral artery occlusion in rats.","volume":"87","author":"Ay","year":"2001"},{"key":"b4","series-title":"Mol Reprod Dev","first-page":"43","article-title":"Potential mechanisms regulating the extracellular activities of basic fibroblast growth factor (FGF-2).","volume":"39","author":"Baird","year":"1994"},{"key":"b5","series-title":"Stroke","first-page":"1616","article-title":"Middle cerebral artery occlusion in the rat by intraluminal suture. Neurological and pathological evaluation of an improved model.","volume":"27","author":"Belayev","year":"1996"},{"key":"b6","series-title":"Stroke","first-page":"609","article-title":"Intravenous basic fibroblast growth factor decreases brain injury resulting from focal ischemia in cats.","volume":"28","author":"Bethel","year":"1997"},{"key":"b7","series-title":"J Cell Biol","first-page":"648","article-title":"Junctions between intimately apposed cell membranes in the vertebrate brain.","volume":"40","author":"Brightman","year":"1969"},{"key":"b8","series-title":"J Cereb Blood Flow Metab","first-page":"186","article-title":"Apoptosis and necrosis after reversible focal ischemia: an in situ DNA fragmentation analysis.","volume":"16","author":"Charriaut-Marlangue","year":"1996"},{"key":"b9","series-title":"Exp Eye Res","first-page":"461","article-title":"Flow of cerebral interstitial fluid as indicated by the removal of extracellular markers from the rat caudate nucleus.","volume":"25","author":"Cserr","year":"1977"},{"key":"b10","series-title":"Exp Neurol","first-page":"50","article-title":"Bulk flow of interstitial fluid after intracranial injection of Blue Dextran 2000.","volume":"45","author":"Cserr","year":"1974"},{"key":"b11","series-title":"Science","first-page":"1208","article-title":"Hypotensive activity of basic fibroblast growth factor.","volume":"254","author":"Cuevas","year":"1991"},{"key":"b12","series-title":"Exp Neurol","first-page":"333","article-title":"Neural transplantation and trophic factors in Parkinsons disease: special reference to chromaffin cell grafting, NGF support from pretransected peripheral nerve, and encapsulated dopamine-secreting cell grafting.","volume":"137","author":"Date","year":"1996"},{"key":"b13","series-title":"Neuroreport","first-page":"1813","article-title":"Chromaffin cell survival from both young and old donors is enhanced by co-grafts of polymer-encapsulated human NGF-secreting cells.","volume":"7","author":"Date","year":"1996"},{"key":"b14","series-title":"J Neurosurg","first-page":"1006","article-title":"Cografting with polymer-encapsulated human nerve growth factor-secreting cells and chromaffin cell survival and behavioral recovery in hemiparkinsonian rats.","volume":"84","author":"Date","year":"1996"},{"key":"b15","series-title":"Exp Neurol","first-page":"10","article-title":"Long-term enhanced chromaffin cell survival and behavioral recovery in hemiparkinsonian rats with co-grafting polymer encapsulated human NGF-secreting cells.","volume":"147","author":"Date","year":"1997"},{"key":"b16","series-title":"Cell Transplant","first-page":"397","article-title":"Grafting of encapsulated genetically modified cells secreting GDNF into the striatum of parkinsonian model rats.","volume":"10","author":"Date","year":"2001"},{"key":"b17","series-title":"Neurosci Biobehav Rev","first-page":"437","article-title":"A novel approach to neural transplantation in Parkinson's disease: use of polymer-encapsulated cell therapy.","volume":"16","author":"Emerich","year":"1992"},{"key":"b18","series-title":"J Cereb Blood Flow Metab","first-page":"953","article-title":"Delayed treatment with intravenous basic fibroblast growth factor reduces infarct size following permanent focal cerebral ischemia in rats.","volume":"15","author":"Fisher","year":"1995"},{"key":"b19","series-title":"Acta Neurochir","first-page":"49","article-title":"Effects of intraventricular infusion of vascular endothelial growth factor on cerebral blood flow, edema, and infarct volume.","volume":"145","author":"Harrigan","year":"2003"},{"key":"b20","series-title":"Am J Physiol","first-page":"H1401","article-title":"bFGF ameliorates focal ischemic injury by blood flow-independent mechanisms in eNOS mutant mice.","volume":"272","author":"Huang","year":"1997"},{"key":"b21","series-title":"J Neurol Sci","first-page":"173","article-title":"Delayed intravenous administration of basic fibroblast growth factor (bFGF) reduces infarct volume in a model of focal cerebral ischemia\/reperfusion in the rat.","volume":"139","author":"Jiang","year":"1996"},{"key":"b22","series-title":"J Cereb Blood Flow Metab","first-page":"542","article-title":"Intracisternal basic fibroblast growth factor (bFGF) enhances behavioral recovery following focal cerebral infarction in the rat.","volume":"16","author":"Kawamata","year":"1996"},{"key":"b23","series-title":"Proc Natl Acad Sci USA","first-page":"8179","article-title":"Intracisternal basic fibroblast growth factor enhances functional recovery and up-regulates the expression of a molecular marker of neuronal sprouting following focal cerebral infarction.","volume":"94","author":"Kawamata","year":"1997"},{"key":"b24","series-title":"J Neurosci Res","first-page":"357","article-title":"Effect of NGF treatment on outcome measures in a rat model of middle cerebral artery occlusion.","volume":"55","author":"Kent","year":"1999"},{"key":"b25","series-title":"Stroke","first-page":"1417","article-title":"Reduction of ischemic brain injury by topical application of glial cell line-derived neurotrophic factor after permanent middle cerebral artery occlusion in rats.","volume":"29","author":"Kitagawa","year":"1998"},{"key":"b26","series-title":"J Cereb Blood Flow Metab","first-page":"1336","article-title":"Adenovirus-mediated gene transfer of glial cell line-derived neurotrophic factor prevents ischemic brain injury after transient middle cerebral artery occlusion in rats.","volume":"19","author":"Kitagawa","year":"1999"},{"key":"b27","series-title":"Ann Neurol","first-page":"451","article-title":"Pretreatment with intraventricular basic fibroblast growth factor decreases infarct size following focal cerebral ischemia in rats.","volume":"35","author":"Koketsu","year":"1994"},{"key":"b28","series-title":"Am J Physiol","first-page":"H1583","article-title":"Basic fibroblast growth factor increases nitric oxide synthase production in bovine endothelial cells.","volume":"269","author":"Kostyk","year":"1995"},{"key":"b29","series-title":"Nucleic Acids Res","first-page":"5201","article-title":"Nucleotide sequence of rat basic fibroblast growth factor cDNA.","volume":"16","author":"Kurokawa","year":"1988"},{"key":"b30","series-title":"Am J Physiol","first-page":"1528","article-title":"Sucrose and inulin space measurements of cerebral cortex in four mammalian species.","volume":"219","author":"Levin","year":"1970"},{"key":"b31","series-title":"Stroke","first-page":"84","article-title":"Reversible middle cerebral artery occlusion without craniectomy in rats.","volume":"20","author":"Longa","year":"1989"},{"key":"b32","series-title":"Brain Res","first-page":"315","article-title":"Basic fibroblast growth factor promotes in vivo cerebral angiogenesis in chronic forebrain ischemia.","volume":"558","author":"Lyons","year":"1991"},{"key":"b33","series-title":"J Neurotrauma","first-page":"3","article-title":"Endogenous neuroprotection factors and traumatic brain injury: mechanisms of action and implications for therapy.","volume":"11","author":"Mattson","year":"1994"},{"key":"b34","series-title":"Exp Neurol","first-page":"169","article-title":"Histological analysis of micro-encapsulated dopamine-secreting cells in agarose\/poly(styrene sulfonic acid) mixed gel xenotransplanted into the brain.","volume":"138","author":"Miyoshi","year":"1996"},{"key":"b35","series-title":"Brain Res","first-page":"354","article-title":"Protective effects of basic fibroblast growth factor against hippocampal neuronal damage following cerebral ischemia in the gerbil.","volume":"605","author":"Nakata","year":"1993"},{"key":"b36","series-title":"Gene","first-page":"193","article-title":"Efficient selection for high-expression transfectants with a novel eukaryotic vector.","volume":"108","author":"Niwa","year":"1991"},{"key":"b37","series-title":"J Cereb Blood Flow Metab","first-page":"221","article-title":"Basic fibroblast growth factor protects against hypoxia-ischemia and NMDA neurotoxicity in neonatal rats.","volume":"13","author":"Nozaki","year":"1993"},{"key":"b38","series-title":"Cell Transplant","first-page":"511","article-title":"Evaluation of intracerebral grafting of dopamine-secreting PC12 cells into allogeneic and xenogeneic brain.","volume":"6","author":"Ono","year":"1997"},{"key":"b39","series-title":"Childs Nerv Syst","first-page":"12","article-title":"Cerebrospinal fluid and extracellular fluid: their relationship to pressure and duration of canine hydrocephalus.","volume":"1","author":"Page","year":"1985"},{"key":"b40","series-title":"Brain Res","first-page":"283","article-title":"Intraventricular infusion of HBGF-2 promotes cerebral angiogenesis in Wistar rat.","volume":"534","author":"Puumala","year":"1990"},{"key":"b41","series-title":"J Cereb Blood Flow Metab","first-page":"70","article-title":"Basic fibroblast growth factor dilates rat pial arterioles.","volume":"14","author":"Rosenblatt","year":"1994"},{"key":"b42","series-title":"Stroke","first-page":"427","article-title":"Synergistic effects of a combination of low-dose basic fibroblast growth factor and citicoline after temporary experimental focal ischemia.","volume":"30","author":"Schabitz","year":"1999"},{"key":"b43","series-title":"J Neurosci Res","first-page":"946","article-title":"Neuroprotective and restorative effects of intrastriatal grafting of encapsulated GDNF-producing cells in a rat model of Parkinson's disease.","volume":"69","author":"Shingo","year":"2002"},{"key":"b44","series-title":"Stroke","first-page":"2154","article-title":"Basic fibroblast growth factor increases regional cerebral blood flow and reduces infarct size after experimental ischemia in a rat model.","volume":"26","author":"Tanaka","year":"1995"},{"key":"b45","series-title":"Stroke","first-page":"2292","article-title":"Effect of basic fibroblast growth factor on experimental focal ischemia studied by diffusion-weighted and perfusion imaging.","volume":"27","author":"Tatlisumak","year":"1996"},{"key":"b46","series-title":"J Neurosci","first-page":"2618","article-title":"Basic and acidic fibroblast growth factors have trophic effects on neurons from multiple CNS regions.","volume":"8","author":"Walicke","year":"1988"},{"key":"b47","series-title":"Metab Brain Dis","first-page":"271","article-title":"Post-occlusion treatment with BDNF reduces infarct size in a model of permanent occlusion of the middle cerebral artery in rat.","volume":"12","author":"Yamashita","year":"1997"},{"key":"b48","series-title":"J Neurosurg","first-page":"874","article-title":"Stereotactic transplantation of a dopamine-producing capsule into the striatum for treatment of Parkinson disease: a preclinical primate study.","volume":"98","author":"Yoshida","year":"2003"},{"key":"b49","series-title":"Gene Therapy","first-page":"942","article-title":"Adenoviral gene transfer of basic fibroblast growth factor promotes angiogenesis in rat brain.","volume":"7","author":"Yukawa","year":"2000"},{"key":"b50","series-title":"Stroke","first-page":"1378","article-title":"Neuroprotection in transient focal brain ischemia after delayed intravenous administration of brain-derived neurotrophic factor conjugated to a blood-brain barrier drug targeting system.","volume":"32","author":"Zhang","year":"2001"}],"container-title":["Journal of Neurosurgery"],"original-title":[],"link":[{"URL":"https:\/\/thejns.org\/view\/journals\/j-neurosurg\/99\/6\/article-p1053.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/thejns.org\/downloadpdf\/journals\/j-neurosurg\/99\/6\/article-p1053.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/thejns.org\/downloadpdf\/journals\/j-neurosurg\/99\/6\/article-p1053.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,3,20]],"date-time":"2023-03-20T00:13:37Z","timestamp":1679271217000},"score":1,"resource":{"primary":{"URL":"https:\/\/thejns.org\/view\/journals\/j-neurosurg\/99\/6\/article-p1053.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,12]]},"references-count":50,"journal-issue":{"issue":"6"},"URL":"https:\/\/doi.org\/10.3171\/jns.2003.99.6.1053","relation":{},"ISSN":["0022-3085"],"issn-type":[{"value":"0022-3085","type":"print"}],"subject":[],"published":{"date-parts":[[2003,12]]}}}