{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,13]],"date-time":"2025-09-13T15:32:27Z","timestamp":1757777547927},"reference-count":52,"publisher":"Journal of Neurosurgery Publishing Group (JNSPG)","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2005,4]]},"abstract":"<jats:p content-type=\"fine-print\"><jats:italic>Object.<\/jats:italic> The aim of this project was to study the perturbations of four smooth-muscle proteins and an extracellular protein, type I collagen, after subarachnoid hemorrhage (SAH) and to examine the possible preventive effects of dexamethasone.<\/jats:p>\n<jats:p content-type=\"fine-print\"><jats:italic>Methods.<\/jats:italic> Using a one-hemorrhage rabbit model, the authors first examined the effects of SAH on the expression of \u03b1-actin, h-caldesmon, vimentin, smoothelin-B, and type I collagen; second, they studied whether post-SAH systemic administration of dexamethasone (three daily injections) corrected the induced alterations. Measurements were obtained at Day 7 post-SAH. The proteins were studied by performing immunohistochemical staining and using a laserscanning confocal microscope.<\/jats:p>\n<jats:p content-type=\"fine-print\">Compared with control (sham-injured) arteries, the density of the media of arteries subjected to SAH was reduced for \u03b1-actin (\u221211%, p = 0.01) and h-caldesmon (\u221215%, p = 0.06) but increased for vimentin (+15%, p = 0.04) and smoothelin-B (+53%, p = 0.04). Among animals in which SAH was induced, arteries in those treated with dexamethasone demonstrated higher values of density for \u03b1-actin (+13%, p = 0.05) and h-caldesmon (+20%, p = 0.01), lower values for vimentin (\u221255%, p = 0.05), and nonsignificantly different values for smoothelin-B. The density of type I collagen in the adventitia decreased significantly after SAH (\u221245%, p = 0.01), but dexamethasone treatment had no effect on this decrease.<\/jats:p>\n<jats:p content-type=\"fine-print\"><jats:italic>Conclusions.<\/jats:italic> The SAH-induced alterations in the density of three of four smooth-muscle proteins were prevented by dexamethasone treatment; two of these proteins\u2014\u03b1-actin and h-caldesmon\u2014are directly related to contraction. This drug may potentially be useful to prevent certain morphological and functional changes in cerebral arteries after SAH.<\/jats:p>","DOI":"10.3171\/jns.2005.102.4.0715","type":"journal-article","created":{"date-parts":[[2009,5,13]],"date-time":"2009-05-13T18:06:30Z","timestamp":1242237990000},"page":"715-720","source":"Crossref","is-referenced-by-count":5,"title":["Dexamethasone preventing contractile and cytoskeletal protein changes in the rabbit basilar artery after subarachnoid hemorrhage"],"prefix":"10.3171","volume":"102","author":[{"given":"Philippe","family":"Gomis","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yves Roger","family":"Tran-Dinh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christine","family":"Sercombe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard","family":"Sercombe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1754","reference":[{"key":"b1","series-title":"Stroke","doi-asserted-by":"publisher","first-page":"212","DOI":"10.1161\/01.STR.32.1.212","article-title":"Quantitative analysis of gene expressions related to inflammation in canine spastic artery after subarachnoid hemorrhage.","volume":"32","author":"Aihara","year":"2001"},{"key":"b2","series-title":"J Mol Endocrinol","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1677\/jme.0.0280069","article-title":"Negative regulation of nuclear factor-kappa B activation and function by glucocorticoids.","volume":"28","author":"Almawi","year":"2002"},{"key":"b3","series-title":"Brain Res","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1016\/S0006-8993(96)01302-9","article-title":"A new mechanism of methylprednisolone and other corticosteroids action demonstrated in vitro: inhibition of a proteinase (calpain) prevents myelin and cytoskeletal protein degradation.","volume":"748","author":"Banik","year":"1997"},{"key":"b4","series-title":"J Cell Biochem","doi-asserted-by":"publisher","first-page":"259","DOI":"10.1002\/jcb.240300307","article-title":"Demonstration of a relationship between talin and p235, a major substrate of the calcium-dependent protease in platelets.","volume":"30","author":"Beckerle","year":"1986"},{"key":"b5","series-title":"Stroke","doi-asserted-by":"publisher","first-page":"472","DOI":"10.1161\/01.STR.18.2.472","article-title":"Functional arterial changes in chronic cerebrovasospasm in monkeys: an in vitro assessment of the contribution to arterial narrowing.","volume":"18","author":"Bevan","year":"1987"},{"key":"b6","series-title":"Biochem J","doi-asserted-by":"publisher","first-page":"233","DOI":"10.1042\/bj3230233","article-title":"Actin is cleaved during constitutive apoptosis.","volume":"323","author":"Brown","year":"1997"},{"key":"b7","series-title":"Neurosurgery","doi-asserted-by":"publisher","first-page":"781","DOI":"10.1227\/00006123-199604000-00029","article-title":"Intracellular calcium, myosin light chain phosphorylation, and contractile force in experimental cerebral vasospasm.","volume":"38","author":"Butler","year":"1996"},{"key":"b8","series-title":"Biochem Biophys Res Commun","doi-asserted-by":"publisher","first-page":"193","DOI":"10.1006\/bbrc.1998.8378","article-title":"Calpain: a protease in search of a function?","volume":"247","author":"Carafoli","year":"1998"},{"key":"b9","series-title":"Stroke","doi-asserted-by":"publisher","first-page":"1021","DOI":"10.1161\/01.STR.20.8.1021","article-title":"Prevention of chronic cerebral vasospasm in dogs with ibuprofen and high-dose methylprednisolone.","volume":"20","author":"Chyatte","year":"1989"},{"key":"b10","series-title":"Neurosurgery","doi-asserted-by":"publisher","first-page":"157","DOI":"10.1227\/00006123-198708000-00004","article-title":"Preliminary report: effects of high dose methylprednisolone on delayed cerebral ischemia in patients at high risk for vasospasm after aneurysmal subarachnoid hemorrhage.","volume":"21","author":"Chyatte","year":"1987"},{"key":"b11","series-title":"J Neurosurg","doi-asserted-by":"publisher","first-page":"925","DOI":"10.3171\/jns.1983.59.6.0925","article-title":"Prevention of chronic experimental cerebral vasospasm with ibuprofen and high-dose methylprednisolone.","volume":"59","author":"Chyatte","year":"1983"},{"key":"b12","series-title":"Biochemistry","doi-asserted-by":"publisher","first-page":"3202","DOI":"10.1021\/bi00359a018","article-title":"Glucocorticoids decrease the synthesis of type I procollagen mRNAs.","volume":"25","author":"Cockayne","year":"1986"},{"key":"b13","series-title":"J Neurosurg","doi-asserted-by":"publisher","first-page":"49","DOI":"10.3171\/jns.1974.41.1.0049","article-title":"Sequential changes of vascular ultrastructure in experimental cerebral vasospasm. Myonecrosis of subarachnoid arteries.","volume":"41","author":"Fein","year":"1974"},{"key":"b14","series-title":"Biol Chem Hoppe Seyler","doi-asserted-by":"publisher","first-page":"1147","DOI":"10.1515\/bchm3.1986.367.2.1147","article-title":"Protein-chemical identification of the major cleavage sites of the Ca2+ proteinase on murine vimentin, the mesenchymal intermediate filament protein.","volume":"367","author":"Fischer","year":"1986"},{"key":"b15","series-title":"J Neurosurg","doi-asserted-by":"publisher","first-page":"92","DOI":"10.3171\/jns.1988.69.1.0092","article-title":"Experimental vasospasm in cultured arterial smooth-muscle cells. Part 1: Contractile and ultrastructural changes caused by oxyhemoglobin.","volume":"69","author":"Fujii","year":"1988"},{"key":"b16","series-title":"Histochem J","doi-asserted-by":"publisher","first-page":"673","DOI":"10.1023\/A:1004115432660","article-title":"Confocal microscopic evidence of decreased alpha-actin expression within rabbit cerebral artery smooth muscle cells after subarachnoid haemorrhage.","volume":"32","author":"Gomis","year":"2000"},{"key":"b17","series-title":"Acta Neurochir","doi-asserted-by":"publisher","first-page":"92","DOI":"10.1007\/BF01404854","article-title":"Expression of intercellular adhesion molecule 1 (ICAM-1) on the cerebral artery following subarachnoid hemorrhage in rats.","volume":"132","author":"Handa","year":"1995"},{"key":"b18","series-title":"J Biol Chem","doi-asserted-by":"publisher","first-page":"4337","DOI":"10.1016\/S0021-9258(17)35666-1","article-title":"Transforming growth factor-\u03b2 stimulates the expression of fibronectin and collagen and their incorporation into the extracellular matrix.","volume":"261","author":"Ignotz","year":"1986"},{"key":"b19","series-title":"Neurosurgery","first-page":"716","article-title":"Procollagen types I and III and transforming growth factor-beta gene expression in the arterial wall after exposure to periarterial blood.","volume":"33","author":"Kasuya","year":"1993"},{"key":"b20","series-title":"J Neurol Sci","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1016\/0022-510X(92)90106-U","article-title":"Effect of high-dose methylprednisolone on anti-oxidant enzymes after experimental SAH.","volume":"111","author":"Lombardi","year":"1992"},{"key":"b21","series-title":"J Neurosurg","doi-asserted-by":"publisher","first-page":"81","DOI":"10.3171\/jns.1992.76.1.0081","article-title":"Cytoskeletal and extracellular matrix proteins in cerebral arteries following subarachnoid hemorrhage in monkeys.","volume":"76","author":"Macdonald","year":"1992"},{"key":"b22","series-title":"J Neurosurg","doi-asserted-by":"publisher","first-page":"626","DOI":"10.3171\/jns.1990.72.4.0626","article-title":"The significance of morphological changes in cerebral arteries after subarachnoid hemorrhage.","volume":"72","author":"Mayberg","year":"1990"},{"key":"b23","series-title":"Endocrine Rev","first-page":"435","article-title":"Molecular control of immune\/inflammatory responses: interactions between nuclear factor-kappa B and steroid receptor-signaling pathways.","volume":"20","author":"McKay","year":"1999"},{"key":"b24","series-title":"J Cell Biochem","doi-asserted-by":"publisher","first-page":"376","DOI":"10.1002\/jcb.240590309","article-title":"Glucocorticoids coordinately regulate type I collagen pro alpha 1 promoter activity through both the glucocorticoid and transforming growth factor beta response elements: a novel mechanism of glucocorticoid regulation of eukaryotic genes.","volume":"59","author":"Meisler","year":"1995"},{"key":"b25","series-title":"J Neurosurg","doi-asserted-by":"publisher","first-page":"111","DOI":"10.3171\/jns.1992.76.1.0111","article-title":"Effects of inhibitors of protein kinase C and calpain in experimental delayed cerebral vasospasm.","volume":"76","author":"Minami","year":"1992"},{"key":"b26","series-title":"Neurosurgery","first-page":"698","article-title":"Immunoblotting of contractile and cytoskeletal proteins of canine basilar artery in vasospasm.","volume":"33","author":"Minami","year":"1993"},{"key":"b27","series-title":"Mol Cell Biol","first-page":"1146","article-title":"Proteolysis of vimentin and desmin by the Ca2+-activated proteinase specific for these intermediate filament proteins.","volume":"3","author":"Nelson","year":"1983"},{"key":"b28","series-title":"J Biol Chem","doi-asserted-by":"publisher","first-page":"5544","DOI":"10.1016\/S0021-9258(19)83811-5","article-title":"Purification and further characterization of the Ca2+-activated proteinase specific for the intermediate filament proteins vimentin and desmin.","volume":"257","author":"Nelson","year":"1982"},{"key":"b29","series-title":"J Biol Chem","doi-asserted-by":"publisher","first-page":"13656","DOI":"10.1016\/S0021-9258(18)89793-9","article-title":"Inhibition of smooth muscle actin-activated myosin Mg2+-ATPase activity by caldesmon.","volume":"259","author":"Ngai","year":"1984"},{"key":"b30","series-title":"Brain Res","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1016\/S0006-8993(00)03120-6","article-title":"Oxyhemoglobin produces apoptosis and necrosis in cultured smooth muscle cells.","volume":"889","author":"Ogihara","year":"2001"},{"key":"b31","series-title":"J Neurosurg","doi-asserted-by":"publisher","first-page":"459","DOI":"10.3171\/jns.1999.91.3.0459","article-title":"Oxyhemoglobin-induced apoptosis in cultured endothelial cells.","volume":"91","author":"Ogihara","year":"1999"},{"key":"b32","series-title":"J Cereb Blood Flow Metab","doi-asserted-by":"publisher","first-page":"1335","DOI":"10.1097\/00004647-199611000-00031","article-title":"Protein synthesis and immunoreactivities of contraction-related proteins in smooth muscle cells of canine basilar artery after experimental subarachnoid hemorrhage.","volume":"16","author":"Oka","year":"1996"},{"key":"b33","series-title":"J Cereb Blood Flow Metab","first-page":"1279","article-title":"Identification of genes differentially expressed in canine vasospastic cerebral arteries after subarachnoid hemorrhage.","volume":"9","author":"Onda","year":"1999"},{"key":"b34","series-title":"J Neurochem","first-page":"1069","article-title":"Subcellular distribution of calpain and calpastatin immunoreactivity and fodrin proteolysis in rabbit hippocampus after hypoxia and glucocorticoid treatment.","volume":"63","author":"Ostwald","year":"1994"},{"key":"b35","series-title":"Stroke","doi-asserted-by":"publisher","first-page":"1219","DOI":"10.1161\/01.STR.29.6.1219","article-title":"Inducible cyclooxygenase expression in canine basilar artery after experimental subarachnoid hemorrhage.","volume":"29","author":"Osuka","year":"1998"},{"key":"b36","series-title":"Int J Biochem Cell Biol","doi-asserted-by":"publisher","first-page":"623","DOI":"10.1016\/S1357-2725(98)00005-3","article-title":"Identification of a glucocorticoid response element in the human transforming growth factor beta 1 gene promoter.","volume":"30","author":"Parrelli","year":"1998"},{"key":"b37","series-title":"Acta Histochem Suppl","first-page":"99","article-title":"Zur Verteilung der intermediaren Filamente in intrakraniellen Gefassen des Menschen.","volume":"42","author":"Roggendorf","year":"1992"},{"key":"b38","series-title":"Stroke","doi-asserted-by":"publisher","first-page":"225","DOI":"10.1161\/01.STR.32.1.225","article-title":"Inhibition of poly(ADP-ribose) polymerase attenuates cerebral vasospasm after subarachnoid hemorrhage in rabbits.","volume":"32","author":"Satoh","year":"2001"},{"key":"b39","series-title":"Neurosurgery","doi-asserted-by":"publisher","first-page":"171","DOI":"10.1227\/00006123-198502000-00008","article-title":"Arterial wall changes in early human vasospasm.","volume":"16","author":"Smith","year":"1985"},{"key":"b40","series-title":"J Biol Chem","doi-asserted-by":"publisher","first-page":"7644","DOI":"10.1016\/S0021-9258(18)32227-0","article-title":"Dexamethasone decreases the amounts of type I procollagen mRNAs in vivo and in fibroblast cell cultures.","volume":"258","author":"Sterling","year":"1983"},{"key":"b41","series-title":"Stroke","doi-asserted-by":"publisher","first-page":"2149","DOI":"10.1161\/01.STR.29.10.2149","article-title":"Myosin light chain phosphorylation and contractile proteins in a canine two-hemorrhage model of subarachnoid hemorrhage.","volume":"29","author":"Sun","year":"1998"},{"key":"b42","series-title":"Arterioscler Thromb Vasc Biol","doi-asserted-by":"publisher","first-page":"665","DOI":"10.1161\/01.ATV.17.4.665","article-title":"Differentiation of smooth muscle cells in human blood vessels as defined by smoothelin, a novel marker for the contractile phenotype.","volume":"17","author":"van der Loop","year":"1997"},{"key":"b43","series-title":"J Cell Biol","doi-asserted-by":"publisher","first-page":"401","DOI":"10.1083\/jcb.134.2.401","article-title":"Smoothelin, a novel cytoskeletal protein specific for smooth muscle cells.","volume":"134","author":"van der Loop","year":"1996"},{"key":"b44","series-title":"Neurosurg Rev","doi-asserted-by":"publisher","first-page":"215","DOI":"10.1007\/BF00310660","article-title":"Longitudinal in vivo and in vitro time-course study of chronic cerebrovasospasm in the rabbit basilar artery.","volume":"14","author":"Vorkapic","year":"1991"},{"key":"b45","series-title":"Trends Neurosci","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1016\/S0166-2236(99)01479-4","article-title":"Calpain and caspase: can you tell the difference?","volume":"23","author":"Wang","year":"2000"},{"key":"b46","series-title":"J Neurosurg","doi-asserted-by":"publisher","first-page":"1083","DOI":"10.3171\/jns.1999.90.6.1083","article-title":"Immediate early gene expression in vascular smooth muscle cells synergistically induced by hemolysate components.","volume":"90","author":"Wang","year":"1999"},{"key":"b47","series-title":"FEBS Lett","doi-asserted-by":"publisher","first-page":"315","DOI":"10.1016\/S0014-5793(97)00207-X","article-title":"Localization of smoothelin in avian smooth muscle and identification of vascular a specific isoform.","volume":"405","author":"Wehrens","year":"1997"},{"key":"b48","series-title":"J Cell Biol","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1083\/jcb.110.1.133","article-title":"Temporal relationships of Factin bundle formation, collagen and fibronectin matrix assembly, and fibronectin receptor expression to wound contraction.","volume":"110","author":"Welch","year":"1990"},{"key":"b49","series-title":"J Neurosurg","doi-asserted-by":"publisher","first-page":"537","DOI":"10.3171\/jns.1993.79.4.0537","article-title":"Calpain-calpastatin system of canine basilar artery in vasospasm.","volume":"79","author":"Yamaura","year":"1993"},{"key":"b50","series-title":"FEBS Lett","doi-asserted-by":"publisher","first-page":"259","DOI":"10.1016\/0014-5793(84)81324-1","article-title":"Degradation of actin and vimentin by calpain II, a Ca2+-dependent cysteine proteinase, in bovine lens.","volume":"170","author":"Yoshida","year":"1984"},{"key":"b51","series-title":"Am J Physiol","first-page":"H1874","article-title":"Mechanisms of hemolysate-induced [Ca2+]i elevation in cerebral smooth muscle cells.","volume":"269","author":"Zhang","year":"1995"},{"key":"b52","series-title":"Surg Neurol","doi-asserted-by":"publisher","first-page":"212","DOI":"10.1016\/S0090-3019(00)00305-0","article-title":"Morphological changes of cerebral penetrating arteries in a canine double hemorrhage model.","volume":"54","author":"Zubkov","year":"2000"}],"container-title":["Journal of Neurosurgery"],"original-title":[],"link":[{"URL":"https:\/\/thejns.org\/view\/journals\/j-neurosurg\/102\/4\/article-p715.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/thejns.org\/downloadpdf\/journals\/j-neurosurg\/102\/4\/article-p715.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/thejns.org\/downloadpdf\/journals\/j-neurosurg\/102\/4\/article-p715.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,7,3]],"date-time":"2022-07-03T14:50:45Z","timestamp":1656859845000},"score":1,"resource":{"primary":{"URL":"https:\/\/thejns.org\/view\/journals\/j-neurosurg\/102\/4\/article-p715.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,4]]},"references-count":52,"journal-issue":{"issue":"4"},"URL":"https:\/\/doi.org\/10.3171\/jns.2005.102.4.0715","relation":{},"ISSN":["0022-3085"],"issn-type":[{"value":"0022-3085","type":"print"}],"subject":[],"published":{"date-parts":[[2005,4]]}}}