{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T05:42:33Z","timestamp":1777873353400,"version":"3.51.4"},"reference-count":33,"publisher":"Frontiers Media SA","issue":"11","license":[{"start":{"date-parts":[[2008,11,1]],"date-time":"2008-11-01T00:00:00Z","timestamp":1225497600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Exp Biol Med (Maywood)"],"published-print":{"date-parts":[[2008,11]]},"abstract":"<jats:p>Several animal and clinical studies have shown that phytoestrogens, plant-derived estrogenic compounds, can be useful in treating postmenopausal osteoporosis. Phytoestrogens and phytoestrogen-containing plants are currently under active investigation for their role in estrogen-related disorders. The present study deals with anti-osteoporotic evaluation of phytoestrogen-rich plant Cuminum cyminum, commonly known as cumin. Adult Sprague-Dawley rats were bilaterally ovariectomized (OVX) and randomly assigned to 3 groups (10 rats\/group). Additional 10 animals were sham operated. OVX and sham control groups were orally administered with vehicle while the other two OVX groups were administered 0.15 mg\/kg estradiol and 1 g\/kg of methanolic extract of Cuminum cyminum fruits (MCC) in two divided doses for 10 weeks. At the end of the study blood, bones and uteri of the animals were collected. Serum was evaluated for calcium, phosphorus, alkaline phosphatase and tartarate resistant acid phosphatase. Bone density, ash density, mineral content and mechanical strength of bones were evaluated. Scanning electron microscopic (SEM) analysis of bones (tibia) was performed. Results were analyzed using ANOVA and Tukeys multiple comparison test. MCC (1 g\/kg, p.o.) significantly reduced urinary calcium excretion and significantly increased calcium content and mechanical strength of bones in comparison to OVX control. It showed greater bone and ash densities and improved microarchitecture of bones in SEM analysis. Unlike estradiol it did not affect body weight gain and weight of atrophic uterus in OVX animals. MCC prevented ovariectomy-induced bone loss in rats with no anabolic effect on atrophic uterus. The osteoprotective effect was comparable with estradiol.<\/jats:p>","DOI":"10.3181\/0803-rm-93","type":"journal-article","created":{"date-parts":[[2008,9,29]],"date-time":"2008-09-29T21:10:29Z","timestamp":1222722629000},"page":"1403-1410","source":"Crossref","is-referenced-by-count":39,"title":["Methanolic Extract of\n                    <i>Cuminum cyminum<\/i>\n                    Inhibits Ovariectomy-Induced Bone Loss in Rats"],"prefix":"10.3389","volume":"233","author":[{"given":"Sarika S.","family":"Shirke","sequence":"first","affiliation":[{"name":"Bombay College of Pharmacy, Kalina, Santacruz (E), Mumbai 400 098, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sanket R.","family":"Jadhav","sequence":"additional","affiliation":[{"name":"Bombay College of Pharmacy, Kalina, Santacruz (E), Mumbai 400 098, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aarti G.","family":"Jagtap","sequence":"additional","affiliation":[{"name":"Bombay College of Pharmacy, Kalina, Santacruz (E), Mumbai 400 098, India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2008,11,1]]},"reference":[{"key":"bibr-R2","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/78.3.593S"},{"key":"bibr-R3","doi-asserted-by":"publisher","DOI":"10.1055\/s-2003-41122"},{"key":"bibr-R4","unstructured":"Rishi RK. Phytoestrogens in health and illness. Ind J Pharmacol 34: 311\u2013320, 2002."},{"key":"bibr-R5","unstructured":"Tham DM, Gardener CD, Haskell WL. Potential health benefits of dietary phytoestrogens: a review of the clinical, epidemiological and mechanistic evidence. J Clin Endocrinol Metab 83:2223\u20132235, 1998."},{"key":"bibr-R6","unstructured":"Brynin R. Soy and its isoflavones: a review of their effects on bone density. Altern Med Rev 7:317\u2013327, 2002."},{"key":"bibr-R9","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-474X(99)00036-3"},{"key":"bibr-R10","unstructured":"Malini T, Vanithakumari G. Estrogenic activity of\n                      Cuminum cyminum\n                      in rats. 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J Biol Chem 193:265\u2013275, 1951.","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"bibr-R22","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1372.019"},{"key":"bibr-R23","doi-asserted-by":"publisher","DOI":"10.1210\/endo-115-2-507"},{"key":"bibr-R24","doi-asserted-by":"publisher","DOI":"10.1271\/bbb.68.1040"},{"key":"bibr-R25","doi-asserted-by":"publisher","DOI":"10.1016\/8756-3282(94)90276-3"},{"key":"bibr-R26","unstructured":"Pytlik M, Janiec W, Cegiela U, Silwinski L. Influence of \u03b1-Eschin on the femoral bone strength in ovariectomized rats. Pol J Pharmacol 51: 511\u2013515, 1999."},{"key":"bibr-R27","doi-asserted-by":"publisher","DOI":"10.1210\/endo-124-1-7"},{"key":"bibr-R28","doi-asserted-by":"crossref","unstructured":"Cai D J, Zhao Y, Glasier J, Cullen D, Barnes S, Turner CH, Wastney M, Weaver CM. Comparative effect of soy protein, soy isoflavones and 17\u03b2 estradiol on bone metabolism in adult ovariectomized rats. 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