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AMP activated protein kinase (AMPK) is a cellular energy \u201cmaster-switch\u201d that is thought to augment glycolysis through phosphorylation and activation phosphofructokinase 2 (PFK2) resulting in increases of the glycolytic stimulator fructose-2,6-bisphosphate (F2,6BP). Previously, Cu deficiency has been shown to augment cerebellar AMPK activation. Cerebella of Cu-adequate (Cu+) and Cu-deficient (Cu\u2212) rat pups were assessed to evaluate if AMPK activation in Cu\u2212 cerebella functioned to enhance PFK2 activation and increase F2,BP concentration. Higher levels of pAMPK were detected in Cu\u2212 cerebella. However, PFK2 activity, mRNA, and protein abundance were not affected by Cu deficiency. Surprisingly, F2,6BP levels were markedly lower in Cu\u2212 cerebella. Lower F2,6BP may be due to inhibition of PFK2 by citrate, as citrate concentration was significantly higher in Cu\u2212 cerebella. Data suggest AMPK activation in Cu\u2212 cerebellum does not augment glycolysis through a PFK2 mechanism. Furthermore, other metabolite data suggest that glycolysis may actually be blunted, since levels of glucose and glucose-6-phosphate were higher in Cu\u2212 cerebella than controls.<\/jats:p>","DOI":"10.3181\/0804-rm-132","type":"journal-article","created":{"date-parts":[[2008,8,14]],"date-time":"2008-08-14T21:40:19Z","timestamp":1218750019000},"page":"1262-1270","source":"Crossref","is-referenced-by-count":12,"title":["Fructose-2,6-Bisphosphate Is Lower in Copper Deficient Rat Cerebellum Despite Higher Content of Phosphorylated AMP-Activated Protein Kinase"],"prefix":"10.3389","volume":"233","author":[{"given":"Anna A.","family":"Gybina","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, University of Minnesota Medical School Duluth, Duluth, Minnesota 55812"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph R.","family":"Prohaska","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, University of Minnesota Medical School Duluth, Duluth, Minnesota 55812"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2008,10,1]]},"reference":[{"key":"bibr-R1","unstructured":"Prohaska JR. 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