{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T01:14:29Z","timestamp":1785892469494,"version":"3.56.0"},"reference-count":12,"publisher":"Frontiers Media SA","issue":"7","license":[{"start":{"date-parts":[[2009,7,1]],"date-time":"2009-07-01T00:00:00Z","timestamp":1246406400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Exp Biol Med (Maywood)"],"published-print":{"date-parts":[[2009,7]]},"abstract":"<jats:p>The ultrasonographic image of an organ is a product of scattering and reflection of high-frequency ultrasound beams by discrete units of tissue. The number of acoustic tissue interfaces and vascularity affects the quantitative characteristics of grey-scale ultrasonographic images. This study was undertaken to examine the influences of scrotal\/testicular integument and blood flow on testicular echotexture parameters in the ram. Serial ultrasonographic images were obtained during surgical castration of 7 Rideau Arcott rams aged 20\u201322 weeks. The first 2 sets of images were taken through the scrotum, prior to and after induction of anaesthesia. The third set was taken through the tunica vaginalis, the fourth set was obtained through the tunica albuginea, the fifth set was taken when the testicular cord and internal blood vessels were clamped, and the final set of images was recorded after allowing the blood to drain from dissected testicles (5 min). All images were then subjected to computerized image analyses and the testicles were processed for histology. The removal of the scrotal skin and tunica vaginalis both resulted in significant ( P &lt; 0.05) increments in numerical pixel values (NPVs) and pixel heterogeneity (standard deviation of pixel values) of the testicular parenchyma. There were no differences ( P &gt; 0.05) in testicular echotexture between images taken just before or after clamping the testicular cord vessels, or after draining. At all stages, NPVs were correlated ( P \u2264 0.10) to the seminiferous tubule (ST) area and the ST lumen area, except for NPVs and the ST lumen area in images obtained through the tunica albuginea ( P = 0.20). We concluded that: 1) attenuation of ultrasound waves by the scrotal skin and tunica vaginalis significantly altered testicular echotexture characteristics; 2) vascular blood flow did not affect the echotextural attributes of the rams\u2019 testes; and 3) NPVs were a good indicator of ST microstructure in situ and ex vivo.<\/jats:p>","DOI":"10.3181\/0812-rm-364","type":"journal-article","created":{"date-parts":[[2009,5,8]],"date-time":"2009-05-08T22:00:08Z","timestamp":1241820008000},"page":"794-801","source":"Crossref","is-referenced-by-count":50,"title":["A Study of Morphological and Haemodynamic Determinants of Testicular Echotexture Characteristics in the Ram"],"prefix":"10.3389","volume":"234","author":[{"given":"Jennifer L.","family":"Giffin","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada N1G 2W1; and Center for Animal Transgenesis and Germ Cell Research, New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, Kennett Square, Pennsylvania 19104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah E.","family":"Franks","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada N1G 2W1; and Center for Animal Transgenesis and Germ Cell Research, New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, Kennett Square, Pennsylvania 19104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose Rafael","family":"Rodriguez-Sosa","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada N1G 2W1; and Center for Animal Transgenesis and Germ Cell Research, New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, Kennett Square, Pennsylvania 19104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ann","family":"Hahnel","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada N1G 2W1; and Center for Animal Transgenesis and Germ Cell Research, New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, Kennett Square, Pennsylvania 19104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pawel M.","family":"Bartlewski","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada N1G 2W1; and Center for Animal Transgenesis and Germ Cell Research, New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, Kennett Square, Pennsylvania 19104"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2009,7,1]]},"reference":[{"key":"bibr-R1","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(96)00190-2"},{"key":"bibr-R2","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2004.12.008"},{"key":"bibr-R3","doi-asserted-by":"publisher","DOI":"10.1097\/01.ju.0000123083.98845.88"},{"key":"bibr-R4","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(97)00194-5"},{"key":"bibr-R5","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(97)00195-7"},{"key":"bibr-R6","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(00)00353-8"},{"key":"bibr-R7","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(94)00014-L"},{"key":"bibr-R9","doi-asserted-by":"publisher","DOI":"10.1080\/01926230290105721"},{"key":"bibr-R10","doi-asserted-by":"publisher","DOI":"10.1007\/s004180050134"},{"key":"bibr-R11","doi-asserted-by":"crossref","unstructured":"Leeson TS, Cookson FB. The mammalian testicular capsule and its muscle elements. 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