{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T03:29:22Z","timestamp":1789615762929,"version":"build-2803163510"},"reference-count":78,"publisher":"Frontiers Media SA","issue":"12","license":[{"start":{"date-parts":[[2009,12,1]],"date-time":"2009-12-01T00:00:00Z","timestamp":1259625600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Exp Biol Med (Maywood)"],"published-print":{"date-parts":[[2009,12]]},"abstract":"<jats:p>Brucella spp. establish an intracellular replicative niche in macrophages, while macrophages attempt to eliminate the bacteria by innate defense mechanisms. Brucella spp. possess similar genomes yet exhibit different macrophage infections. Few B. melitensis and B. neotomae enter macrophages with intracellular adaptation occurring over 4\u20138 hr. Conversely, B. ovis are readily ingested by macrophages and exhibit a persistent plateau of infection. Evaluating early macrophage interaction with Brucella spp. allows discovery of host entry and intracellular translocation mechanisms. Microarray analysis of macrophage transcriptional response following a 4 hr infection by different Brucella spp. revealed common macrophage genes altered in expression compared to uninfected macrophages. Macrophage infection with three different Brucella spp. provokes a common innate immune theme with increased transcript levels of chemokines and defense response genes and decreased transcript levels of GTPase signaling and cytoskeletal function that may affect trafficking of Brucella containing vesicles. For example, transcript levels of genes associated with chemotaxis ( IL-1\u03b2 , MIP-1\u03b1), cytokine regulation ( Socs3) and defense ( Fas, Tnf) were increased, while transcript levels of genes associated with vesicular trafficking ( Rab3d) and lysosomal associated enzymes ( prosaposin) were decreased. Genes with altered macrophage transcript levels among Brucella spp. infections may correlate with species specific host defenses and intracellular survival strategies. Depending on the infecting Brucella species, gene ontology categorization identified genes differentially involved in cell growth and maintenance, endopeptidase inhibitor activity and G-protein mediated signaling. Examples of decreased gene expression in B. melitensis infection but not other Brucella spp. were growth arrest ( Gas2), immunoglobulin receptor ( Fc\u03b3 rI) and chemokine receptor ( Cxcr4) genes, suggesting opposing effects on intracellular functions.<\/jats:p>","DOI":"10.3181\/0904-rm-124","type":"journal-article","created":{"date-parts":[[2009,11,24]],"date-time":"2009-11-24T13:57:49Z","timestamp":1259071069000},"page":"1450-1467","source":"Crossref","is-referenced-by-count":35,"title":["<i>Brucella melitensis, B. neotomae<\/i>\n                    and\n                    <i>B. ovis<\/i>\n                    Elicit Common and Distinctive Macrophage Defense Transcriptional Responses"],"prefix":"10.3389","volume":"234","author":[{"given":"Jill","family":"Covert","sequence":"first","affiliation":[{"name":"Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, Wisconsin 53706; and Kimron Veterinary Institute, Department of Bacteriology, Beit Dagan 50250, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela J.","family":"Mathison","sequence":"additional","affiliation":[{"name":"Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, Wisconsin 53706; and Kimron Veterinary Institute, Department of Bacteriology, Beit Dagan 50250, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda","family":"Eskra","sequence":"additional","affiliation":[{"name":"Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, Wisconsin 53706; and Kimron Veterinary Institute, Department of Bacteriology, Beit Dagan 50250, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Menachem","family":"Banai","sequence":"additional","affiliation":[{"name":"Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, Wisconsin 53706; and Kimron Veterinary Institute, Department of Bacteriology, Beit Dagan 50250, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary","family":"Splitter","sequence":"additional","affiliation":[{"name":"Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, Wisconsin 53706; and Kimron Veterinary Institute, Department of Bacteriology, Beit Dagan 50250, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2009,12,1]]},"reference":[{"key":"bibr-R2","doi-asserted-by":"publisher","DOI":"10.1615\/CritRevImmunol.v26.i5.30"},{"key":"bibr-R3","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.8.2715-2726.2005"},{"key":"bibr-R4","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.15.5040-5051.2004"},{"key":"bibr-R5","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.12.7004-7012.2002"},{"key":"bibr-R6","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.18.6025-6031.2004"},{"key":"bibr-R7","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.3.1125-1133.2003"},{"key":"bibr-R8","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20030088"},{"key":"bibr-R9","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01998-05"},{"key":"bibr-R10","doi-asserted-by":"publisher","DOI":"10.1002\/jlb.56.2.174"},{"key":"bibr-R11","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.61.1.124-134.1993"},{"key":"bibr-R12","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.6.3995-4006.2001"},{"key":"bibr-R13","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.12.7736-7742.2001"},{"key":"bibr-R15","doi-asserted-by":"publisher","DOI":"10.1002\/sim.1548"},{"key":"bibr-R18","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.091062498"},{"key":"bibr-R19","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(06)11009-5"},{"key":"bibr-R20","doi-asserted-by":"publisher","DOI":"10.2144\/03342mt01"},{"key":"bibr-R21","doi-asserted-by":"crossref","unstructured":"Rozen S, Skaletsky H. 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