{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T13:05:06Z","timestamp":1791119106155,"version":"4.1.0"},"reference-count":24,"publisher":"Centers for Disease Control and Prevention (CDC)","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Emerg. Infect. Dis."],"DOI":"10.3201\/eid0810.020393","type":"journal-article","created":{"date-parts":[[2012,6,27]],"date-time":"2012-06-27T14:37:21Z","timestamp":1340807841000},"page":"1178-1182","source":"Crossref","is-referenced-by-count":108,"title":["Evaluation and Validation of a Real-Time Polymerase Chain Reaction Assay for Rapid Identification of\n                    <i>Bacillus anthracis<\/i>"],"prefix":"10.3201","volume":"8","author":[{"given":"Alex R.","family":"Hoffmaster","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard F.","family":"Meyer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael P.","family":"Bowen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chung K.","family":"Marston","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robbin S.","family":"Weyant","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathy","family":"Thurman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharon L.","family":"Messenger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erin E.","family":"Minor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonas M.","family":"Winchell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Max V.","family":"Rasmussen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruce R.","family":"Newton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. Todd","family":"Parker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William E.","family":"Morrill","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nancy","family":"McKinney","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gwen A.","family":"Barnett","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James J.","family":"Sejvar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John A.","family":"Jernigan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bradley A.","family":"Perkins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tanja","family":"Popovic","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1822","published-online":{"date-parts":[[2002,10]]},"reference":[{"key":"key-10.3201\/eid0810.020393-201206270400-R1","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1046\/j.1472-765x.2001.00989.x","article-title":"Detection of anthrax spores from the air by real-time PCR.","volume":"33","author":"Makino","year":"2001","journal-title":"Lett Appl Microbiol"},{"key":"key-10.3201\/eid0810.020393-201206270400-R2","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1128\/JCM.31.3.547-551.1993","article-title":"Direct detection of Bacillus anthracis DNA in animals by polymerase chain reaction.","volume":"31","author":"Makino","year":"1993","journal-title":"J Clin Microbiol"},{"key":"key-10.3201\/eid0810.020393-201206270400-R3","doi-asserted-by":"publisher","first-page":"218","DOI":"10.1046\/j.1365-2672.1999.00908.x","article-title":"Fluorescent detection techniques for real-time multiplex strand specific detection of Bacillus anthracis using rapid PCR.","volume":"87","author":"Lee","year":"1999","journal-title":"J Appl Microbiol"},{"key":"key-10.3201\/eid0810.020393-201206270400-R4","first-page":"21","article-title":"Bacillus anthracis but not always anthrax.","volume":"72","author":"Turnbull","year":"1992","journal-title":"J Appl Bacteriol"},{"key":"key-10.3201\/eid0810.020393-201206270400-R5","doi-asserted-by":"crossref","first-page":"6509","DOI":"10.1128\/JB.181.20.6509-6515.1999","article-title":"Sequence and organization of pXO1, the large Bacillus anthracis plasmid harboring the anthrax toxin genes.","volume":"181","author":"Okinaka","year":"1999","journal-title":"J Bacteriol"},{"key":"key-10.3201\/eid0810.020393-201206270400-R6","doi-asserted-by":"publisher","first-page":"261","DOI":"10.1046\/j.1365-2672.1999.00883.x","article-title":"Sequence, assembly and analysis of pXO1 and pXO2.","volume":"87","author":"Okinaka","year":"1999","journal-title":"J Appl Microbiol"},{"key":"key-10.3201\/eid0810.020393-201206270400-R7","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1128\/IAI.49.2.291-297.1985","article-title":"Demonstration of a capsule plasmid in Bacillus anthracis.","volume":"49","author":"Green","year":"1985","journal-title":"Infect Immun"},{"key":"key-10.3201\/eid0810.020393-201206270400-R8","doi-asserted-by":"publisher","first-page":"134","DOI":"10.1128\/JB.184.1.134-141.2002","article-title":"Bacillus anthracis pXO1 plasmid sequence conservation among closely related bacterial species.","volume":"184","author":"Pannucci","year":"2002","journal-title":"J Bacteriol"},{"key":"key-10.3201\/eid0810.020393-201206270400-R9","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1111\/j.1574-6968.1996.tb08548.x","article-title":"Identification and characterization of Bacillus anthracis by multiplex PCR analysis of sequences on plasmids pXO1 and pXO2 and chromosomal DNA.","volume":"145","author":"Ramisse","year":"1996","journal-title":"FEMS Microbiol Lett"},{"key":"key-10.3201\/eid0810.020393-201206270400-R10","doi-asserted-by":"publisher","first-page":"224","DOI":"10.1046\/j.1365-2672.1999.00874.x","article-title":"The Ba813 chromosomal DNA sequence effectively traces the whole Bacillus anthracis community.","volume":"87","author":"Ramisse","year":"1999","journal-title":"J Appl Microbiol"},{"key":"key-10.3201\/eid0810.020393-201206270400-R11","doi-asserted-by":"crossref","first-page":"3412","DOI":"10.1128\/JCM.36.11.3412-3414.1998","article-title":"Molecular characterization of Bacillus strains involved in outbreaks of anthrax in France in 1997.","volume":"36","author":"Patra","year":"1998","journal-title":"J Clin Microbiol"},{"key":"key-10.3201\/eid0810.020393-201206270400-R12","doi-asserted-by":"publisher","first-page":"1224","DOI":"10.1073\/pnas.95.3.1224","article-title":"PCR analysis of tissue samples from the 1979 Sverdlovsk anthrax victims: the presence of multiple Bacillus anthracis strains in different victims.","volume":"95","author":"Jackson","year":"1998","journal-title":"Proc Natl Acad Sci U S A"},{"key":"key-10.3201\/eid0810.020393-201206270400-R13","doi-asserted-by":"publisher","first-page":"3720","DOI":"10.1128\/AEM.67.8.3720-3727.2001","article-title":"Utilization of the rpoB gene as a specific chromosomal marker for real-time PCR detection of Bacillus anthracis.","volume":"67","author":"Qi","year":"2001","journal-title":"Appl Environ Microbiol"},{"key":"key-10.3201\/eid0810.020393-201206270400-R14","doi-asserted-by":"publisher","first-page":"1179","DOI":"10.1016\/S0140-6736(00)02769-0","article-title":"Public-health preparedness for biological terrorism in the USA.","volume":"356","author":"Khan","year":"2000","journal-title":"Lancet"},{"key":"key-10.3201\/eid0810.020393-201206270400-R15","doi-asserted-by":"publisher","first-page":"2928","DOI":"10.1128\/JB.182.10.2928-2936.2000","article-title":"Multiple-locus variable-number tandem repeat analysis reveals genetic relationships within Bacillus anthracis.","volume":"182","author":"Keim","year":"2000","journal-title":"J Bacteriol"},{"key":"key-10.3201\/eid0810.020393-201206270400-R16","doi-asserted-by":"publisher","first-page":"992","DOI":"10.1016\/0002-9343(60)90079-6","article-title":"An epidemic of inhalation anthrax, the first in the twentieth century.","volume":"29","author":"Plotkin","year":"1960","journal-title":"Am J Med"},{"key":"key-10.3201\/eid0810.020393-201206270400-R17","doi-asserted-by":"publisher","first-page":"1111","DOI":"10.3201\/eid0810.020393","article-title":"Molecular Subtyping of Bacillus anthracis and the 2001 Bioterrorism-Associated Anthrax Outbreak, United States.","volume":"8","author":"Hoffmaster","year":"2002","journal-title":"Emerg Infect Dis"},{"key":"key-10.3201\/eid0810.020393-201206270400-R18","unstructured":"Logan NA, Turnbull PC. Bacillus and recently derived genera. In: Murray PR, editor. Manual of clinical microbiology. Washington (DC): ASM Press; 2001. p. 357\u201369."},{"key":"key-10.3201\/eid0810.020393-201206270400-R19","doi-asserted-by":"publisher","first-page":"2627","DOI":"10.1128\/AEM.66.6.2627-2630.2000","article-title":"Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis\u2014one species on the basis of genetic evidence.","volume":"66","author":"Helgason","year":"2000","journal-title":"Appl Environ Microbiol"},{"key":"key-10.3201\/eid0810.020393-201206270400-R20","doi-asserted-by":"publisher","first-page":"343","DOI":"10.1099\/00207713-41-3-343","article-title":"Comparative analysis of Bacillus anthracis, Bacillus cereus, and related species on the basis of reverse transcriptase sequencing of 16S rRNA.","volume":"41","author":"Ash","year":"1991","journal-title":"Int J Syst Bacteriol"},{"key":"key-10.3201\/eid0810.020393-201206270400-R21","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1111\/j.1348-0421.1978.tb00414.x","article-title":"Deoxyribonucleic acid relatedness between Bacillus anthracis, Bacillus cereus and Bacillus thuringiensis.","volume":"22","author":"Kaneko","year":"1978","journal-title":"Microbiol Immunol"},{"key":"key-10.3201\/eid0810.020393-201206270400-R22","doi-asserted-by":"publisher","first-page":"4863","DOI":"10.1128\/AEM.67.10.4863-4873.2001","article-title":"Fluorescent amplified fragment length polymorphism analysis of Norwegian Bacillus cereus and Bacillus thuringiensis soil isolates.","volume":"67","author":"Ticknor","year":"2001","journal-title":"Appl Environ Microbiol"},{"key":"key-10.3201\/eid0810.020393-201206270400-R23","first-page":"77","article-title":"Rapid-cycle real-time PCR: a revolution for clinical microbiology.","volume":"68","author":"Cockerill","year":"2002","journal-title":"ASM News"},{"key":"key-10.3201\/eid0810.020393-201206270400-R24","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1101\/gr.4.6.357","article-title":"Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization.","volume":"4","author":"Livak","year":"1995","journal-title":"PCR Methods Appl"}],"container-title":["Emerging Infectious Diseases"],"original-title":[],"link":[{"URL":"https:\/\/wwwnc.cdc.gov\/eid\/article\/8\/10\/02-0393_article","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,25]],"date-time":"2024-11-25T21:41:50Z","timestamp":1732570910000},"score":1,"resource":{"primary":{"URL":"http:\/\/wwwnc.cdc.gov\/eid\/article\/8\/10\/02-0393_article.htm"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,10]]},"references-count":24,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2002,10]]}},"URL":"https:\/\/doi.org\/10.3201\/eid0810.020393","relation":{},"ISSN":["1080-6040","1080-6059"],"issn-type":[{"value":"1080-6040","type":"print"},{"value":"1080-6059","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,10]]}}}