{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T09:11:16Z","timestamp":1790932276924,"version":"4.1.0"},"reference-count":24,"publisher":"Centers for Disease Control and Prevention (CDC)","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Emerg. Infect. Dis."],"published-print":{"date-parts":[[2020,6]]},"DOI":"10.3201\/eid2606.200516","type":"journal-article","created":{"date-parts":[[2020,3,11]],"date-time":"2020-03-11T16:20:37Z","timestamp":1583943637000},"page":"1266-1273","source":"Crossref","is-referenced-by-count":537,"title":["Severe Acute Respiratory Syndrome Coronavirus 2 from Patient with Coronavirus Disease, United States"],"prefix":"10.3201","volume":"26","author":[{"given":"Jennifer","family":"Harcourt","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Azaibi","family":"Tamin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyan","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shifaq","family":"Kamili","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Senthil K.","family":"Sakthivel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janna","family":"Murray","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krista","family":"Queen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Tao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clinton R.","family":"Paden","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Uehara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haibin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cynthia","family":"Goldsmith","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hannah A.","family":"Bullock","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijuan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brett","family":"Whitaker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian","family":"Lynch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rashi","family":"Gautam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Craig","family":"Schindewolf","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kumari G.","family":"Lokugamage","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dionna","family":"Scharton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica A.","family":"Plante","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Divya","family":"Mirchandani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven G.","family":"Widen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krishna","family":"Narayanan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinji","family":"Makino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas G.","family":"Ksiazek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth S.","family":"Plante","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott C.","family":"Weaver","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen","family":"Lindstrom","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suxiang","family":"Tong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vineet D.","family":"Menachery","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalie J.","family":"Thornburg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1822","reference":[{"key":"key-10.3201\/eid2606.200516-202005181602-R1","doi-asserted-by":"publisher","first-page":"929","DOI":"10.1056\/NEJMoa2001191","article-title":"Washington State 2019-nCoV Case Investigation Team. First case of 2019 novel coronavirus in the United States.","volume":"382","author":"Holshue","year":"2020","journal-title":"N Engl J Med"},{"key":"key-10.3201\/eid2606.200516-202005181602-R2","doi-asserted-by":"publisher","first-page":"727","DOI":"10.1056\/NEJMoa2001017","article-title":"A novel coronavirus from patients with pneumonia in China, 2019.","volume":"382","author":"Zhu","year":"2020","journal-title":"N Engl J Med"},{"key":"key-10.3201\/eid2606.200516-202005181602-R3","doi-asserted-by":"publisher","first-page":"565","DOI":"10.1016\/S0140-6736(20)30251-8","article-title":"Genomic characterisation and epidemiology of 2019 novel coronavirus: implications for virus origins and receptor binding.","volume":"395","author":"Lu","year":"2020","journal-title":"Lancet"},{"key":"key-10.3201\/eid2606.200516-202005181602-R4","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1080\/22221751.2020.1719902","article-title":"Genomic characterization of the 2019 novel human-pathogenic coronavirus isolated from a patient with atypical pneumonia after visiting Wuhan.","volume":"9","author":"Chan","year":"2020","journal-title":"Emerg Microbes Infect"},{"key":"key-10.3201\/eid2606.200516-202005181602-R5","doi-asserted-by":"publisher","first-page":"NEJMoa2001316","DOI":"10.1056\/NEJMoa2001316","article-title":"Early transmission dynamics in Wuhan, China, of novel coronavirus-infected pneumonia.","author":"Li","year":"2020","journal-title":"N Engl J Med"},{"key":"key-10.3201\/eid2606.200516-202005181602-R6","doi-asserted-by":"publisher","first-page":"507","DOI":"10.1016\/S0140-6736(20)30211-7","article-title":"Epidemiological and clinical characteristics of 99 cases of 2019 novel coronavirus pneumonia in Wuhan, China: a descriptive study.","volume":"395","author":"Chen","year":"2020","journal-title":"Lancet"},{"key":"key-10.3201\/eid2606.200516-202005181602-R7","doi-asserted-by":"publisher","first-page":"514","DOI":"10.1016\/S0140-6736(20)30154-9","article-title":"A familial cluster of pneumonia associated with the 2019 novel coronavirus indicating person-to-person transmission: a study of a family cluster.","volume":"395","author":"Chan","year":"2020","journal-title":"Lancet"},{"key":"key-10.3201\/eid2606.200516-202005181602-R8","doi-asserted-by":"publisher","first-page":"JVI.00127-20","DOI":"10.1128\/JVI.00127-20","article-title":"Receptor recognition by novel coronavirus from Wuhan: An analysis based on decade-long structural studies of SARS.","author":"Wan","year":"2020","journal-title":"J Virol"},{"key":"key-10.3201\/eid2606.200516-202005181602-R9","doi-asserted-by":"publisher","first-page":"3885","DOI":"10.1128\/JVI.02520-12","article-title":"Release of severe acute respiratory syndrome coronavirus nuclear import block enhances host transcription in human lung cells.","volume":"87","author":"Sims","year":"2013","journal-title":"J Virol"},{"key":"key-10.3201\/eid2606.200516-202005181602-R10","doi-asserted-by":"publisher","first-page":"e00165","DOI":"10.1128\/mBio.00165-13","article-title":"Cell host response to infection with novel human coronavirus EMC predicts potential antivirals and important differences with SARS coronavirus.","volume":"4","author":"Josset","year":"2013","journal-title":"MBio"},{"key":"key-10.3201\/eid2606.200516-202005181602-R11","doi-asserted-by":"publisher","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","article-title":"Trimmomatic: a flexible trimmer for Illumina sequence data.","volume":"30","author":"Bolger","year":"2014","journal-title":"Bioinformatics"},{"key":"key-10.3201\/eid2606.200516-202005181602-R12","doi-asserted-by":"publisher","first-page":"1117","DOI":"10.1101\/gr.089532.108","article-title":"ABySS: a parallel assembler for short read sequence data.","volume":"19","author":"Simpson","year":"2009","journal-title":"Genome Res"},{"key":"key-10.3201\/eid2606.200516-202005181602-R13","doi-asserted-by":"publisher","first-page":"1754","DOI":"10.1093\/bioinformatics\/btp324","article-title":"Fast and accurate short read alignment with Burrows-Wheeler transform.","volume":"25","author":"Li","year":"2009","journal-title":"Bioinformatics"},{"key":"key-10.3201\/eid2606.200516-202005181602-R14","doi-asserted-by":"publisher","first-page":"24","DOI":"10.1038\/nbt.1754","article-title":"Integrative genomics viewer.","volume":"29","author":"Robinson","year":"2011","journal-title":"Nat Biotechnol"},{"key":"key-10.3201\/eid2606.200516-202005181602-R15","doi-asserted-by":"publisher","first-page":"343","DOI":"10.1016\/j.jviromet.2006.06.029","article-title":"Copious production of SARS-CoV nucleocapsid protein employing codon optimized synthetic gene.","volume":"137","author":"Das","year":"2006","journal-title":"J Virol Methods"},{"key":"key-10.3201\/eid2606.200516-202005181602-R16","doi-asserted-by":"publisher","first-page":"104742","DOI":"10.1016\/j.antiviral.2020.104742","article-title":"The spike glycoprotein of the new coronavirus 2019-nCoV contains a furin-like cleavage site absent in CoV of the same clade.","volume":"176","author":"Coutard","year":"2020","journal-title":"Antiviral Res"},{"key":"key-10.3201\/eid2606.200516-202005181602-R17","doi-asserted-by":"publisher","first-page":"2407","DOI":"10.1002\/j.1460-2075.1992.tb05305.x","article-title":"Influenza virus hemagglutinin with multibasic cleavage site is activated by furin, a subtilisin-like endoprotease.","volume":"11","author":"Stieneke-Gr\u00f6ber","year":"1992","journal-title":"EMBO J"},{"key":"key-10.3201\/eid2606.200516-202005181602-R18","doi-asserted-by":"publisher","first-page":"450","DOI":"10.1038\/nature02145","article-title":"Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus.","volume":"426","author":"Li","year":"2003","journal-title":"Nature"},{"key":"key-10.3201\/eid2606.200516-202005181602-R19","doi-asserted-by":"publisher","first-page":"1743","DOI":"10.1093\/infdis\/jit123","article-title":"Differential cell line susceptibility to the emerging novel human betacoronavirus 2c EMC\/2012: implications for disease pathogenesis and clinical manifestation.","volume":"207","author":"Chan","year":"2013","journal-title":"J Infect Dis"},{"key":"key-10.3201\/eid2606.200516-202005181602-R20","doi-asserted-by":"publisher","first-page":"3196","DOI":"10.1128\/JCM.42.7.3196-3206.2004","article-title":"Discovery of novel human and animal cells infected by the severe acute respiratory syndrome coronavirus by replication-specific multiplex reverse transcription-PCR.","volume":"42","author":"Gillim-Ross","year":"2004","journal-title":"J Clin Microbiol"},{"key":"key-10.3201\/eid2606.200516-202005181602-R21","doi-asserted-by":"publisher","first-page":"128","DOI":"10.3201\/eid1201.050496","article-title":"SARS-associated coronavirus replication in cell lines.","volume":"12","author":"Kaye","year":"2006","journal-title":"Emerg Infect Dis"},{"key":"key-10.3201\/eid2606.200516-202005181602-R22","doi-asserted-by":"publisher","first-page":"914","DOI":"10.3201\/eid1005.030827","article-title":"Domestic poultry and SARS coronavirus, southern China.","volume":"10","author":"Swayne","year":"2004","journal-title":"Emerg Infect Dis"},{"key":"key-10.3201\/eid2606.200516-202005181602-R23","doi-asserted-by":"publisher","first-page":"16157","DOI":"10.1073\/pnas.1311542110","article-title":"Reverse genetics with a full-length infectious cDNA of the Middle East respiratory syndrome coronavirus.","volume":"110","author":"Scobey","year":"2013","journal-title":"Proc Natl Acad Sci U S A"},{"key":"key-10.3201\/eid2606.200516-202005181602-R24","doi-asserted-by":"publisher","first-page":"12995","DOI":"10.1073\/pnas.1735582100","article-title":"Reverse genetics with a full-length infectious cDNA of severe acute respiratory syndrome coronavirus.","volume":"100","author":"Yount","year":"2003","journal-title":"Proc Natl Acad Sci U S A"}],"container-title":["Emerging Infectious Diseases"],"original-title":[],"link":[{"URL":"https:\/\/wwwnc.cdc.gov\/eid\/article\/26\/6\/20-0516_article","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,30]],"date-time":"2024-11-30T17:34:11Z","timestamp":1732988051000},"score":1,"resource":{"primary":{"URL":"http:\/\/wwwnc.cdc.gov\/eid\/article\/26\/6\/20-0516_article.htm"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6]]},"references-count":24,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2020,6]]}},"URL":"https:\/\/doi.org\/10.3201\/eid2606.200516","relation":{},"ISSN":["1080-6040","1080-6059"],"issn-type":[{"value":"1080-6040","type":"print"},{"value":"1080-6059","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,6]]}}}