{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T11:04:54Z","timestamp":1783595094139,"version":"3.55.0"},"reference-count":33,"publisher":"Centers for Disease Control and Prevention (CDC)","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Emerg. Infect. Dis."],"published-print":{"date-parts":[[2021,5]]},"DOI":"10.3201\/eid2705.190121","type":"journal-article","created":{"date-parts":[[2021,4,27]],"date-time":"2021-04-27T10:32:53Z","timestamp":1619519573000},"page":"1427-1437","source":"Crossref","is-referenced-by-count":14,"title":["Engineered NS1 for Sensitive, Specific Zika Virus Diagnosis from Patient Serology"],"prefix":"10.3201","volume":"27","author":[{"given":"Thai Leong","family":"Yap","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shin Yee","family":"Hong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun Hui","family":"Soh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lekha","family":"Ravichandraprabhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanessa W.X.","family":"Lim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hsi-Min","family":"Chan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tommy Z.X.","family":"Ong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying Ping","family":"Chua","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shi En","family":"Koh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huajing","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yee Sin","family":"Leo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jackie Y.","family":"Ying","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1822","reference":[{"key":"key-10.3201\/eid2705.190121-202104201232-R1","doi-asserted-by":"publisher","first-page":"601","DOI":"10.1056\/NEJMp1600297","article-title":"Zika virus in the Americas\u2014yet another arbovirus threat.","volume":"374","author":"Fauci","year":"2016","journal-title":"N Engl J Med"},{"key":"key-10.3201\/eid2705.190121-202104201232-R2","doi-asserted-by":"publisher","first-page":"389","DOI":"10.1016\/0035-9203(81)90100-0","article-title":"Zika virus, a cause of fever in Central Java, Indonesia.","volume":"75","author":"Olson","year":"1981","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"key-10.3201\/eid2705.190121-202104201232-R3","doi-asserted-by":"publisher","first-page":"411","DOI":"10.4269\/ajtmh.1969.18.411","article-title":"Isolation of Zika virus from Aedes aegypti mosquitoes in Malaysia.","volume":"18","author":"Marchette","year":"1969","journal-title":"Am J Trop Med Hyg"},{"key":"key-10.3201\/eid2705.190121-202104201232-R4","doi-asserted-by":"publisher","first-page":"442","DOI":"10.1016\/0035-9203(56)90090-6","article-title":"Zika virus infection experimentally induced in a human volunteer.","volume":"50","author":"Bearcroft","year":"1956","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"key-10.3201\/eid2705.190121-202104201232-R5","doi-asserted-by":"publisher","first-page":"335","DOI":"10.1016\/0035-9203(64)90201-9","article-title":"Zlka virus infection in man.","volume":"58","author":"Simpson","year":"1964","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"key-10.3201\/eid2705.190121-202104201232-R6","doi-asserted-by":"publisher","first-page":"2536","DOI":"10.1056\/NEJMoa0805715","article-title":"Zika virus outbreak on Yap Island, Federated States of Micronesia.","volume":"360","author":"Duffy","year":"2009","journal-title":"N Engl J Med"},{"key":"key-10.3201\/eid2705.190121-202104201232-R7","unstructured":"European Centre for Disease Prevention and Control. Rapid risk assessment: Zika virus epidemic in the Americas: potential association with microcephaly and Guillain-Barr\u00e9 syndrome. Solna, Sweden: The Centre; 2015. p. 1\u201314."},{"key":"key-10.3201\/eid2705.190121-202104201232-R8","doi-asserted-by":"publisher","first-page":"1981","DOI":"10.1056\/NEJMsr1604338","article-title":"Zika virus and birth defects\u2014reviewing the evidence for causality.","volume":"374","author":"Rasmussen","year":"2016","journal-title":"N Engl J Med"},{"key":"key-10.3201\/eid2705.190121-202104201232-R9","doi-asserted-by":"publisher","first-page":"267","DOI":"10.1038\/nature18296","article-title":"The Brazilian Zika virus strain causes birth defects in experimental models.","volume":"534","author":"Cugola","year":"2016","journal-title":"Nature"},{"key":"key-10.3201\/eid2705.190121-202104201232-R10","doi-asserted-by":"publisher","first-page":"e1002203","DOI":"10.1371\/journal.pmed.1002203","article-title":"Zika virus infection as a cause of congenital brain abnormalities and Guillain-Barr\u00e9 syndrome: systematic review.","volume":"14","author":"Krauer","year":"2017","journal-title":"PLoS Med"},{"key":"key-10.3201\/eid2705.190121-202104201232-R11","doi-asserted-by":"publisher","first-page":"1513","DOI":"10.1056\/NEJMoa1605564","article-title":"Guillain-Barr\u00e9 syndrome associated with Zika virus infection in Colombia.","volume":"375","author":"Parra","year":"2016","journal-title":"N Engl J Med"},{"key":"key-10.3201\/eid2705.190121-202104201232-R12","doi-asserted-by":"publisher","first-page":"1531","DOI":"10.1016\/S0140-6736(16)00562-6","article-title":"Guillain-Barr\u00e9 Syndrome outbreak associated with Zika virus infection in French Polynesia: a case-control study.","volume":"387","author":"Cao-Lormeau","year":"2016","journal-title":"Lancet"},{"key":"key-10.3201\/eid2705.190121-202104201232-R13","doi-asserted-by":"publisher","first-page":"60","DOI":"10.5858\/arpa.2016-0406-SA","article-title":"Laboratory diagnosis of Zika virus infection.","volume":"141","author":"Landry","year":"2017","journal-title":"Arch Pathol Lab Med"},{"key":"key-10.3201\/eid2705.190121-202104201232-R14","unstructured":"US Department of Health and Human Services, US Food and Drug Administration. Emergency use authorizations for medical devices [cited 2018 Dec 1]. https:\/\/www.fda.gov\/medicaldevices\/safety\/emergencysituations\/ucm161496.htm"},{"key":"key-10.3201\/eid2705.190121-202104201232-R15","doi-asserted-by":"publisher","first-page":"475","DOI":"10.15585\/mmwr.mm6518e2","article-title":"Comparison of test results for Zika virus RNA in urine, serum, and saliva specimens from persons with travel-associated Zika virus disease\u2014Florida, 2016.","volume":"65","author":"Bingham","year":"2016","journal-title":"MMWR Morb Mortal Wkly Rep"},{"key":"key-10.3201\/eid2705.190121-202104201232-R16","doi-asserted-by":"publisher","first-page":"880","DOI":"10.2471\/BLT.16.175950","article-title":"Assay optimization for molecular detection of Zika virus.","volume":"94","author":"Corman","year":"2016","journal-title":"Bull World Health Organ"},{"key":"key-10.3201\/eid2705.190121-202104201232-R17","doi-asserted-by":"publisher","first-page":"535","DOI":"10.1128\/JCM.01232-16","article-title":"Zika virus testing considerations: lessons learned from the first 80 real-time reverse transcription-PCR-positive cases diagnosed in New York state.","volume":"55","author":"St George","year":"2017","journal-title":"J Clin Microbiol"},{"key":"key-10.3201\/eid2705.190121-202104201232-R18","unstructured":"Centers for Disease Control and Prevention. Testing guidance: new Zika and dengue testing guidance [cited 2018 Dec 1]. https:\/\/www.cdc.gov\/zika\/hc-providers\/testing-guidance.html"},{"key":"key-10.3201\/eid2705.190121-202104201232-R19","doi-asserted-by":"publisher","first-page":"543","DOI":"10.15585\/mmwr.mm6521e1","article-title":"Interim guidance for interpretation of Zika virus antibody test results.","volume":"65","author":"Rabe","year":"2016","journal-title":"MMWR Morb Mortal Wkly Rep"},{"key":"key-10.3201\/eid2705.190121-202104201232-R20","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1099\/0022-1317-70-1-37","article-title":"Antigenic relationships between flaviviruses as determined by cross-neutralization tests with polyclonal antisera.","volume":"70","author":"Calisher","year":"1989","journal-title":"J Gen Virol"},{"key":"key-10.3201\/eid2705.190121-202104201232-R21","doi-asserted-by":"publisher","first-page":"2127","DOI":"10.1128\/JCM.00580-17","article-title":"Serologic testing for Zika virus: comparison of three Zika virus IgM-screening enzyme-linked immunosorbent assays and initial laboratory experiences.","volume":"55","author":"Granger","year":"2017","journal-title":"J Clin Microbiol"},{"key":"key-10.3201\/eid2705.190121-202104201232-R22","doi-asserted-by":"publisher","first-page":"1232","DOI":"10.3201\/eid1408.080287","article-title":"Genetic and serologic properties of Zika virus associated with an epidemic, Yap State, Micronesia, 2007.","volume":"14","author":"Lanciotti","year":"2008","journal-title":"Emerg Infect Dis"},{"key":"key-10.3201\/eid2705.190121-202104201232-R23","doi-asserted-by":"publisher","first-page":"611","DOI":"10.1016\/j.jinf.2017.03.007","article-title":"Clinical features of patients with Zika and dengue virus co-infection in Singapore.","volume":"74","author":"Chia","year":"2017","journal-title":"J Infect"},{"key":"key-10.3201\/eid2705.190121-202104201232-R24","doi-asserted-by":"publisher","first-page":"61","DOI":"10.3347\/kjp.2018.56.1.61","article-title":"Development of a rapid diagnostic test kit to detect IgG\/IgM antibody against Zika virus using monoclonal antibodies to the envelope and non-structural protein 1 of the virus.","volume":"56","author":"Kim","year":"2018","journal-title":"Korean J Parasitol"},{"key":"key-10.3201\/eid2705.190121-202104201232-R25","doi-asserted-by":"publisher","first-page":"e3171","DOI":"10.1371\/journal.pntd.0003171","article-title":"Evaluation of commercially available diagnostic tests for the detection of dengue virus NS1 antigen and anti-dengue virus IgM antibody.","volume":"8","author":"Hunsperger","year":"2014","journal-title":"PLoS Negl Trop Dis"},{"key":"key-10.3201\/eid2705.190121-202104201232-R26","doi-asserted-by":"publisher","first-page":"e280","DOI":"10.1371\/journal.pntd.0000280","article-title":"Evaluation of two new commercial tests for the diagnosis of acute dengue virus infection using NS1 antigen detection in human serum.","volume":"2","author":"Dussart","year":"2008","journal-title":"PLoS Negl Trop Dis"},{"key":"key-10.3201\/eid2705.190121-202104201232-R27","unstructured":"Centers for Disease Control and Prevention. Zika MAC-ELISA [cited 2018 Dec 1]. https:\/\/www.cdc.gov\/zika\/pdfs\/zika-mac-elisa-instructions-for-use.pdf"},{"key":"key-10.3201\/eid2705.190121-202104201232-R28","doi-asserted-by":"publisher","first-page":"8384","DOI":"10.1073\/pnas.1704984114","article-title":"Antibody-based assay discriminates Zika virus infection from other flaviviruses.","volume":"114","author":"Balmaseda","year":"2017","journal-title":"Proc Natl Acad Sci U S A"},{"key":"key-10.3201\/eid2705.190121-202104201232-R29","doi-asserted-by":"publisher","first-page":"30426","DOI":"10.2807\/1560-7917.ES.2016.21.50.30426","article-title":"Serodiagnosis of Zika virus (ZIKV) infections by a novel NS1-based ELISA devoid of cross-reactivity with dengue virus antibodies: a multicohort study of assay performance, 2015 to 2016.","volume":"21","author":"Steinhagen","year":"2016","journal-title":"Euro Surveill"},{"key":"key-10.3201\/eid2705.190121-202104201232-R30","unstructured":"US Department of Health and Human Services, US Food and Drug Administration. FDA warns health care providers against relying solely on Zika virus serological IgM assay results; reminds them to wait for confirmatory test results before making patient management decisions: FDA safety communication; 2016 [cited 2018 Dec 1]. http:\/\/wayback.archive-it.org\/7993\/20170722113716\/https:\/\/www.fda.gov\/MedicalDevices\/Safety\/AlertsandNotices\/ucm534515.htm"},{"key":"key-10.3201\/eid2705.190121-202104201232-R31","doi-asserted-by":"crossref","first-page":"e01504","DOI":"10.1128\/JCM.01504-17","article-title":"Development of envelope protein antigens to serologically differentiate Zika virus infection from dengue virus infection.","volume":"56","author":"Premkumar","year":"2018","journal-title":"J Clin Microbiol"},{"key":"key-10.3201\/eid2705.190121-202104201232-R32","doi-asserted-by":"publisher","first-page":"e99","DOI":"10.1038\/emi.2017.87","article-title":"Specific detection of dengue and Zika virus antibodies using envelope proteins with mutations in the conserved fusion loop.","volume":"6","author":"Rockstroh","year":"2017","journal-title":"Emerg Microbes Infect"},{"key":"key-10.3201\/eid2705.190121-202104201232-R33","doi-asserted-by":"publisher","first-page":"eaan1589","DOI":"10.1126\/scitranslmed.aan1589","article-title":"Rapid antigen tests for dengue virus serotypes and Zika virus in patient serum.","volume":"9","author":"Bosch","year":"2017","journal-title":"Sci Transl Med"}],"container-title":["Emerging Infectious Diseases"],"original-title":[],"link":[{"URL":"https:\/\/wwwnc.cdc.gov\/eid\/article\/27\/5\/19-0121_article","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,30]],"date-time":"2024-11-30T17:51:31Z","timestamp":1732989091000},"score":1,"resource":{"primary":{"URL":"https:\/\/wwwnc.cdc.gov\/eid\/article\/27\/5\/19-0121_article.htm"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,5]]},"references-count":33,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2021,5]]}},"URL":"https:\/\/doi.org\/10.3201\/eid2705.190121","relation":{},"ISSN":["1080-6040","1080-6059"],"issn-type":[{"value":"1080-6040","type":"print"},{"value":"1080-6059","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,5]]}}}