{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T03:20:43Z","timestamp":1792552843455,"version":"4.5.0"},"reference-count":46,"publisher":"Centers for Disease Control and Prevention (CDC)","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Emerg. Infect. Dis."],"published-print":{"date-parts":[[2023,1]]},"DOI":"10.3201\/eid3001.221588","type":"journal-article","created":{"date-parts":[[2023,12,20]],"date-time":"2023-12-20T18:27:27Z","timestamp":1703096847000},"source":"Crossref","is-referenced-by-count":47,"title":["Molecular Evolution and Increasing Macrolide Resistance of\n                    <i>Bordetella pertussis<\/i>\n                    , Shanghai, China, 2016\u20132022"],"prefix":"10.3201","volume":"30","author":[{"given":"Pan","family":"Fu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinlan","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaxier","family":"Nijiati","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijun","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gangfen","family":"Yan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoping","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaowen","family":"Zhai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuanqing","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1822","reference":[{"key":"key-10.3201\/eid3001.221588-202312131132-R1","doi-asserted-by":"publisher","first-page":"ftv079","DOI":"10.1093\/femspd\/ftv079","article-title":"Bordetella filamentous hemagglutinin and fimbriae: critical adhesins with unrealized vaccine potential.","volume":"73","author":"Scheller","year":"2015","journal-title":"Pathog Dis"},{"key":"key-10.3201\/eid3001.221588-202312131132-R2","doi-asserted-by":"publisher","first-page":"6327","DOI":"10.1016\/j.vaccine.2015.09.040","article-title":"Genetic diversity and population dynamics of Bordetella pertussis in China between 1950-2007.","volume":"33","author":"Xu","year":"2015","journal-title":"Vaccine"},{"key":"key-10.3201\/eid3001.221588-202312131132-R3","doi-asserted-by":"publisher","first-page":"680","DOI":"10.1128\/JCM.01838-08","article-title":"Changes in genetic diversity of the Bordetella pertussis population in the United Kingdom between 1920 and 2006 reflect vaccination coverage and emergence of a single dominant clonal type.","volume":"47","author":"Litt","year":"2009","journal-title":"J Clin Microbiol"},{"key":"key-10.3201\/eid3001.221588-202312131132-R4","doi-asserted-by":"publisher","first-page":"593","DOI":"10.1097\/INF.0b013e31820eaf88","article-title":"Seroprevalence of Bordetella pertussis antibody in children and adolescents in China.","volume":"30","author":"Wang","year":"2011","journal-title":"Pediatr Infect Dis J"},{"key":"key-10.3201\/eid3001.221588-202312131132-R5","doi-asserted-by":"publisher","first-page":"685","DOI":"10.1017\/S0950268813000071","article-title":"Pertussis resurgence: waning immunity and pathogen adaptation - two sides of the same coin.","volume":"142","author":"Mooi","year":"2014","journal-title":"Epidemiol Infect"},{"key":"key-10.3201\/eid3001.221588-202312131132-R6","doi-asserted-by":"publisher","first-page":"370","DOI":"10.1097\/INF.0000000000002160","article-title":"Bordetella pertussis infection in infants and young children in Shanghai, China, 2016\u20132017: clinical features, genotype variations of antigenic genes and macrolides resistance.","volume":"38","author":"Fu","year":"2019","journal-title":"Pediatr Infect Dis J"},{"key":"key-10.3201\/eid3001.221588-202312131132-R7","doi-asserted-by":"publisher","first-page":"670","DOI":"10.1128\/IAI.66.2.670-675.1998","article-title":"Polymorphism in the Bordetella pertussis virulence factors P.69\/pertactin and pertussis toxin in The Netherlands: temporal trends and evidence for vaccine-driven evolution.","volume":"66","author":"Mooi","year":"1998","journal-title":"Infect Immun"},{"key":"key-10.3201\/eid3001.221588-202312131132-R8","doi-asserted-by":"publisher","first-page":"3133","DOI":"10.1128\/IAI.67.6.3133-3134.1999","article-title":"Variation in the Bordetella pertussis virulence factors pertussis toxin and pertactin in vaccine strains and clinical isolates in Finland.","volume":"67","author":"Mooi","year":"1999","journal-title":"Infect Immun"},{"key":"key-10.3201\/eid3001.221588-202312131132-R9","doi-asserted-by":"publisher","first-page":"1402","DOI":"10.1086\/315881","article-title":"Polymorphism in Bordetella pertussis pertactin and pertussis toxin virulence factors in the United States, 1935-1999.","volume":"182","author":"Cassiday","year":"2000","journal-title":"J Infect Dis"},{"key":"key-10.3201\/eid3001.221588-202312131132-R10","doi-asserted-by":"publisher","first-page":"3681","DOI":"10.1128\/JCM.43.8.3681-3687.2005","article-title":"Strain variation among Bordetella pertussis isolates in finland, where the whole-cell pertussis vaccine has been used for 50 years.","volume":"43","author":"Elomaa","year":"2005","journal-title":"J Clin Microbiol"},{"key":"key-10.3201\/eid3001.221588-202312131132-R11","doi-asserted-by":"publisher","first-page":"2069","DOI":"10.1099\/13500872-145-8-2069","article-title":"Antigenic variants in Bordetella pertussis strains isolated from vaccinated and unvaccinated children.","volume":"145","author":"Mastrantonio","year":"1999","journal-title":"Microbiology (Reading)"},{"key":"key-10.3201\/eid3001.221588-202312131132-R12","doi-asserted-by":"publisher","first-page":"S119","DOI":"10.1016\/j.jinf.2013.09.022","article-title":"Bordetella pertussis: why is it still circulating?","volume":"68","author":"Guiso","year":"2014","journal-title":"J Infect"},{"key":"key-10.3201\/eid3001.221588-202312131132-R13","doi-asserted-by":"publisher","first-page":"3920","DOI":"10.1128\/iai.63.10.3920-3926.1995","article-title":"Pertussis toxin export genes are regulated by the ptx promoter and may be required for efficient translation of ptx mRNA in Bordetella pertussis.","volume":"63","author":"Baker","year":"1995","journal-title":"Infect Immun"},{"key":"key-10.3201\/eid3001.221588-202312131132-R14","doi-asserted-by":"publisher","first-page":"1206","DOI":"10.3201\/eid1508.081511","article-title":"Bordetella pertussis strains with increased toxin production associated with pertussis resurgence.","volume":"15","author":"Mooi","year":"2009","journal-title":"Emerg Infect Dis"},{"key":"key-10.3201\/eid3001.221588-202312131132-R15","doi-asserted-by":"publisher","first-page":"901617","DOI":"10.3389\/fmicb.2022.901617","article-title":"A cross-sectional study revealing the emergence of erythromycin-resistant Bordetella pertussis carrying ptxP3 alleles in China.","volume":"13","author":"Wu","year":"2022","journal-title":"Front Microbiol"},{"key":"key-10.3201\/eid3001.221588-202312131132-R16","doi-asserted-by":"publisher","first-page":"1570","DOI":"10.3390\/antibiotics11111570","article-title":"Macrolide resistance in Bordetella pertussis: current situation and future challenges.","volume":"11","author":"Ivaska","year":"2022","journal-title":"Antibiotics (Basel)"},{"key":"key-10.3201\/eid3001.221588-202312131132-R17","doi-asserted-by":"publisher","first-page":"56","DOI":"10.1016\/j.ijid.2017.07.015","article-title":"Molecular epidemiology of Bordetella pertussis in Cambodia determined by direct genotyping of clinical specimens.","volume":"62","author":"Moriuchi","year":"2017","journal-title":"Int J Infect Dis"},{"key":"key-10.3201\/eid3001.221588-202312131132-R18","doi-asserted-by":"publisher","first-page":"3438","DOI":"10.1016\/j.vaccine.2011.02.070","article-title":"Appearance of Fim3 and ptxP3-Bordetella pertussis strains, in two regions of Sweden with different vaccination programs.","volume":"29","author":"Advani","year":"2011","journal-title":"Vaccine"},{"key":"key-10.3201\/eid3001.221588-202312131132-R19","doi-asserted-by":"publisher","first-page":"2011","DOI":"10.1099\/00221287-148-7-2011","article-title":"Changes in the Dutch Bordetella pertussis population in the first 20 years after the introduction of whole-cell vaccines.","volume":"148","author":"van Loo","year":"2002","journal-title":"Microbiology (Reading)"},{"key":"key-10.3201\/eid3001.221588-202312131132-R20","doi-asserted-by":"publisher","first-page":"e0138941","DOI":"10.1371\/journal.pone.0138941","article-title":"Variation in Bordetella pertussis susceptibility to erythromycin and virulence-related genotype changes in China (1970\u20132014).","volume":"10","author":"Yang","year":"2015","journal-title":"PLoS One"},{"key":"key-10.3201\/eid3001.221588-202312131132-R21","doi-asserted-by":"publisher","first-page":"1093","DOI":"10.1080\/14760584.2020.1831916","article-title":"The epidemic of erythromycin-resistant Bordetella pertussis with limited genome variation associated with pertussis resurgence in China.","volume":"19","author":"Yao","year":"2020","journal-title":"Expert Rev Vaccines"},{"key":"key-10.3201\/eid3001.221588-202312131132-R22","doi-asserted-by":"publisher","first-page":"4823","DOI":"10.1038\/s41598-021-84409-0","article-title":"Rapid and simple SNP genotyping for Bordetella pertussis epidemic strain MT27 based on a multiplexed single-base extension assay.","volume":"11","author":"Kamachi","year":"2021","journal-title":"Sci Rep"},{"key":"key-10.3201\/eid3001.221588-202312131132-R23","doi-asserted-by":"publisher","first-page":"461","DOI":"10.1080\/22221751.2019.1587315","article-title":"Genomic epidemiology of erythromycin-resistant Bordetella pertussis in China.","volume":"8","author":"Xu","year":"2019","journal-title":"Emerg Microbes Infect"},{"key":"key-10.3201\/eid3001.221588-202312131132-R24","doi-asserted-by":"publisher","first-page":"1211","DOI":"10.1007\/s10096-016-2655-3","article-title":"Direct molecular typing of Bordetella pertussis from nasopharyngeal specimens in China in 2012-2013.","volume":"35","author":"Du","year":"2016","journal-title":"Eur J Clin Microbiol Infect Dis"},{"key":"key-10.3201\/eid3001.221588-202312131132-R25","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1186\/s12941-021-00458-3","article-title":"Molecular epidemiology of Bordetella pertussis and analysis of vaccine antigen genes from clinical isolates from Shenzhen, China.","volume":"20","author":"Wu","year":"2021","journal-title":"Ann Clin Microbiol Antimicrob"},{"key":"key-10.3201\/eid3001.221588-202312131132-R26","doi-asserted-by":"publisher","first-page":"3000605221078782","DOI":"10.1177\/03000605221078782","article-title":"Macrolide susceptibility and molecular characteristics of Bordetella pertussis.","volume":"50","author":"Zhang","year":"2022","journal-title":"J Int Med Res"},{"key":"key-10.3201\/eid3001.221588-202312131132-R27","doi-asserted-by":"publisher","first-page":"100098","DOI":"10.1016\/j.lanwpc.2021.100098","article-title":"Emerging macrolide resistance in Bordetella pertussis in mainland China: Findings and warning from the global pertussis initiative.","volume":"8","author":"Feng","year":"2021","journal-title":"Lancet Reg Health West Pac"},{"key":"key-10.3201\/eid3001.221588-202312131132-R28","doi-asserted-by":"publisher","first-page":"263","DOI":"10.1016\/j.jgar.2022.10.007","article-title":"Genotyping and macrolide-resistant mutation of Bordetella pertussis in East and South-East Asia.","volume":"31","author":"Koide","year":"2022","journal-title":"J Glob Antimicrob Resist"},{"key":"key-10.3201\/eid3001.221588-202312131132-R29","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1016\/j.ijid.2023.01.005","article-title":"Emergence and spread of MT28 ptxP3 allele macrolide-resistant Bordetella pertussis from 2021 to 2022 in China.","volume":"128","author":"Fu","year":"2023","journal-title":"Int J Infect Dis"},{"key":"key-10.3201\/eid3001.221588-202312131132-R30","doi-asserted-by":"publisher","first-page":"O825","DOI":"10.1111\/1469-0691.12671","article-title":"High prevalence of erythromycin-resistant Bordetella pertussis in Xi\u2019an, China.","volume":"20","author":"Wang","year":"2014","journal-title":"Clin Microbiol Infect"},{"key":"key-10.3201\/eid3001.221588-202312131132-R31","doi-asserted-by":"publisher","first-page":"1994","DOI":"10.1128\/JCM.40.6.1994-2001.2002","article-title":"Multilocus sequence typing of Bordetella pertussis based on surface protein genes.","volume":"40","author":"van Loo","year":"2002","journal-title":"J Clin Microbiol"},{"key":"key-10.3201\/eid3001.221588-202312131132-R32","doi-asserted-by":"publisher","first-page":"5496","DOI":"10.1128\/JB.186.16.5496-5505.2004","article-title":"Multiple-locus variable-number tandem repeat analysis of Dutch Bordetella pertussis strains reveals rapid genetic changes with clonal expansion during the late 1990s.","volume":"186","author":"Schouls","year":"2004","journal-title":"J Bacteriol"},{"key":"key-10.3201\/eid3001.221588-202312131132-R33","doi-asserted-by":"publisher","first-page":"379","DOI":"10.1111\/jam.13011","article-title":"Portable lysis apparatus for rapid single-step DNA extraction of Bacillus subtilis.","volume":"120","author":"Lim","year":"2016","journal-title":"J Appl Microbiol"},{"key":"key-10.3201\/eid3001.221588-202312131132-R34","doi-asserted-by":"publisher","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","article-title":"Trimmomatic: a flexible trimmer for Illumina sequence data.","volume":"30","author":"Bolger","year":"2014","journal-title":"Bioinformatics"},{"key":"key-10.3201\/eid3001.221588-202312131132-R35","doi-asserted-by":"publisher","first-page":"455","DOI":"10.1089\/cmb.2012.0021","article-title":"SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing.","volume":"19","author":"Bankevich","year":"2012","journal-title":"J Comput Biol"},{"key":"key-10.3201\/eid3001.221588-202312131132-R36","doi-asserted-by":"publisher","first-page":"1221","DOI":"10.1038\/s41564-022-01182-0","article-title":"Outbreak dynamics of foodborne pathogen Vibrio parahaemolyticus over a seventeen year period implies hidden reservoirs.","volume":"7","author":"Yang","year":"2022","journal-title":"Nat Microbiol"},{"key":"key-10.3201\/eid3001.221588-202312131132-R37","doi-asserted-by":"crossref","unstructured":"Delcher AL, Salzberg SL, Phillippy AM. Using MUMmer to identify similar regions in large sequence sets. Curr Protoc Bioinformatics. 2003 Feb;Chapter 10:Unit 10.3.","DOI":"10.1002\/0471250953.bi1003s00"},{"key":"key-10.3201\/eid3001.221588-202312131132-R38","doi-asserted-by":"publisher","first-page":"e000056","DOI":"10.1099\/mgen.0.000056","article-title":"SNP-sites: rapid efficient extraction of SNPs from multi-FASTA alignments.","volume":"2","author":"Page","year":"2016","journal-title":"Microb Genom"},{"key":"key-10.3201\/eid3001.221588-202312131132-R39","doi-asserted-by":"publisher","first-page":"4453","DOI":"10.1093\/bioinformatics\/btz305","article-title":"RAxML-NG: a fast, scalable and user-friendly tool for maximum likelihood phylogenetic inference.","volume":"35","author":"Kozlov","year":"2019","journal-title":"Bioinformatics"},{"key":"key-10.3201\/eid3001.221588-202312131132-R40","doi-asserted-by":"publisher","first-page":"E260","DOI":"10.1111\/1469-0691.12161","article-title":"High-resolution melting analysis for the detection of two erythromycin-resistant Bordetella pertussis strains carried by healthy schoolchildren in China.","volume":"19","author":"Zhang","year":"2013","journal-title":"Clin Microbiol Infect"},{"key":"key-10.3201\/eid3001.221588-202312131132-R41","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1007\/s12519-019-00308-5","article-title":"Clinical characteristics, molecular epidemiology and antimicrobial susceptibility of pertussis among children in southern China.","volume":"16","author":"Zhang","year":"2020","journal-title":"World J Pediatr"},{"key":"key-10.3201\/eid3001.221588-202312131132-R42","doi-asserted-by":"publisher","first-page":"615","DOI":"10.1186\/s12879-020-05332-9","article-title":"The global prevalence ptxP3 lineage of Bordetella pertussis was rare in young children with the co-purified aPV vaccination: a 5\u2009years retrospective study.","volume":"20","author":"Wang","year":"2020","journal-title":"BMC Infect Dis"},{"key":"key-10.3201\/eid3001.221588-202312131132-R43","doi-asserted-by":"publisher","first-page":"7174","DOI":"10.1016\/j.vaccine.2012.10.009","article-title":"Acellular pertussis vaccines in China.","volume":"30","author":"Wang","year":"2012","journal-title":"Vaccine"},{"key":"key-10.3201\/eid3001.221588-202312131132-R44","first-page":"33","article-title":"Recombinant hybrid proteins from pertactin type 1 and 2 of Bordetella pertussis are more immunogenic in mice than the original molecules.","volume":"31","author":"Quintana-V\u00e1zquez","year":"2014","journal-title":"Biotecnol Apl"},{"key":"key-10.3201\/eid3001.221588-202312131132-R45","doi-asserted-by":"publisher","first-page":"3967","DOI":"10.1016\/j.vaccine.2016.06.052","article-title":"Better colonisation of newly emerged Bordetella pertussis in the co-infection mouse model study.","volume":"34","author":"Safarchi","year":"2016","journal-title":"Vaccine"},{"key":"key-10.3201\/eid3001.221588-202312131132-R46","doi-asserted-by":"publisher","first-page":"e18014","DOI":"10.1371\/journal.pone.0018014","article-title":"Studies on Prn variation in the mouse model and comparison with epidemiological data.","volume":"6","author":"van Gent","year":"2011","journal-title":"PLoS One"}],"container-title":["Emerging Infectious Diseases"],"original-title":[],"link":[{"URL":"https:\/\/wwwnc.cdc.gov\/eid\/article\/30\/1\/22-1588_article","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,29]],"date-time":"2024-11-29T13:36:04Z","timestamp":1732887364000},"score":1,"resource":{"primary":{"URL":"https:\/\/wwwnc.cdc.gov\/eid\/article\/30\/1\/22-1588_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,1]]},"references-count":46,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2023,1]]}},"URL":"https:\/\/doi.org\/10.3201\/eid3001.221588","relation":{},"ISSN":["1080-6040","1080-6059"],"issn-type":[{"value":"1080-6040","type":"print"},{"value":"1080-6059","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,1]]}}}