{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T16:04:07Z","timestamp":1784304247689,"version":"3.55.0"},"reference-count":12,"publisher":"Centers for Disease Control and Prevention (CDC)","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Emerg. Infect. Dis."],"published-print":{"date-parts":[[2026,8]]},"DOI":"10.3201\/eid3208.251737","type":"journal-article","created":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T15:18:52Z","timestamp":1784301532000},"source":"Crossref","is-referenced-by-count":0,"title":["Detection of Congenital Syphilis via Digital PCR and Next-Generation Sequencing, Colombia"],"prefix":"10.3201","volume":"32","author":[{"given":"Ana M.","family":"Bossa-Castro","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Narda M. Olarte","family":"Escobar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ismael Alberto","family":"Valderrama M\u00e1rquez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deisy","family":"Abril","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis","family":"Fuentes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zayda Lorena","family":"Corredor-Rozo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruth Liliana","family":"L\u00f3pez Cruz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharon Hassbleidy","family":"Ochoa Ram\u00edrez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Alejandro Mojica","family":"Madera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro Jos\u00e9","family":"Ramos Cabrera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge Eliecer","family":"Castellanos Corredor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nidia Aydee Garay","family":"Bernal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Mar\u00eda","family":"G\u00f3mez Puentes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos Alberto","family":"Morales Pertuz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier","family":"Escobar-P\u00e9rez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1822","reference":[{"key":"key-10.3201\/eid3208.251737-202607171118-R1","unstructured":"World Health Organization. WHO guidelines for the treatment of\n                      Treponema pallidum\n                      (syphilis). Geneva; The Organization: 2016."},{"key":"key-10.3201\/eid3208.251737-202607171118-R2","doi-asserted-by":"publisher","first-page":"1","DOI":"10.15585\/mmwr.rr7004a1","article-title":"Sexually transmitted infections treatment guidelines, 2021.","volume":"70","author":"Workowski","year":"2021","journal-title":"MMWR Recomm Rep"},{"key":"key-10.3201\/eid3208.251737-202607171118-R3","unstructured":"Ministry of Health and Social Protection. Evidence-based clinical practice guideline (CPG) for the comprehensive care of gestational and congenital syphilis [in Spanish]. Bogata: The Ministry; 2014."},{"key":"key-10.3201\/eid3208.251737-202607171118-R4","unstructured":"National Institute of Health. Public health surveillance protocol for gestational and congenital syphilis [in Spanish]. Bogata: The Institute; 2015."},{"key":"key-10.3201\/eid3208.251737-202607171118-R5","unstructured":"Centers for Disease Control and Prevention. Congenital syphilis (CS) case investigation and report form, 2013 [cited 2025 Oct 27]. https:\/\/stacks.cdc.gov\/view\/61366"},{"key":"key-10.3201\/eid3208.251737-202607171118-R6","doi-asserted-by":"publisher","first-page":"1","DOI":"10.15585\/mmwr.rr7301a1","article-title":"CDC laboratory recommendations for syphilis testing, United States, 2024.","volume":"73","author":"Papp","year":"2024","journal-title":"MMWR Recomm Rep"},{"key":"key-10.3201\/eid3208.251737-202607171118-R7","doi-asserted-by":"publisher","first-page":"479","DOI":"10.1136\/sti.79.6.479","article-title":"Use of PCR in the diagnosis of early syphilis in the United Kingdom.","volume":"79","author":"Palmer","year":"2003","journal-title":"Sex Transm Infect"},{"key":"key-10.3201\/eid3208.251737-202607171118-R8","doi-asserted-by":"publisher","first-page":"931","DOI":"10.3390\/diagnostics14090931","article-title":"Present and future applications of digital PCR in infectious diseases diagnosis.","volume":"14","author":"Sancha Dominguez","year":"2024","journal-title":"Diagnostics (Basel)"},{"key":"key-10.3201\/eid3208.251737-202607171118-R9","doi-asserted-by":"publisher","first-page":"1469440","DOI":"10.3389\/fcimb.2025.1469440","article-title":"Clinical utility of nanopore-targeted sequencing for diagnosing and treating pulmonary infectious diseases from bronchoalveolar lavage fluid.","volume":"15","author":"Li","year":"2025","journal-title":"Front Cell Infect Microbiol"},{"key":"key-10.3201\/eid3208.251737-202607171118-R10","doi-asserted-by":"publisher","first-page":"e01153","DOI":"10.1128\/mBio.01153-18","article-title":"Long-term in vitro culture of the syphilis spirochete Treponema pallidum subsp. pallidum.","volume":"9","author":"Edmondson","year":"2018","journal-title":"MBio"},{"key":"key-10.3201\/eid3208.251737-202607171118-R11","doi-asserted-by":"publisher","first-page":"453","DOI":"10.1128\/iai.59.1.453-456.1991","article-title":"Median infective dose of Treponema pallidum determined in a highly susceptible guinea pig strain.","volume":"59","author":"Wicher","year":"1991","journal-title":"Infect Immun"},{"key":"key-10.3201\/eid3208.251737-202607171118-R12","doi-asserted-by":"publisher","first-page":"463","DOI":"10.1093\/pch\/5.8.463","article-title":"Congenital syphilis: a guide to diagnosis and management.","volume":"5","author":"Arnold","year":"2000","journal-title":"Paediatr Child Health"}],"container-title":["Emerging Infectious Diseases"],"original-title":[],"deposited":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T15:18:53Z","timestamp":1784301533000},"score":1,"resource":{"primary":{"URL":"https:\/\/wwwnc.cdc.gov\/eid\/article\/32\/8\/25-1737_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8]]},"references-count":12,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8]]}},"URL":"https:\/\/doi.org\/10.3201\/eid3208.251737","relation":{},"ISSN":["1080-6040","1080-6059"],"issn-type":[{"value":"1080-6040","type":"print"},{"value":"1080-6059","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8]]}}}