{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T23:16:30Z","timestamp":1780960590660,"version":"3.54.1"},"reference-count":20,"publisher":"Siapec Servizi Srl","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathologica"],"DOI":"10.32074\/1591-951x-1117","type":"journal-article","created":{"date-parts":[[2025,4,10]],"date-time":"2025-04-10T07:39:06Z","timestamp":1744270746000},"page":"10-17","source":"Crossref","is-referenced-by-count":4,"title":["Morpho-molecular approach (NGS plus digital PCR) in diagnosis of malignant biliary strictures"],"prefix":"10.32074","volume":"117","author":[{"given":"Francesco","family":"Vasuri","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisa","family":"Albertini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucia","family":"Miranda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thais","family":"Maloberti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefano","family":"Chillotti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Coluccelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanni","family":"Tallini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonia","family":"D\u2019Errico","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dario","family":"de Biase","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"21774","published-online":{"date-parts":[[2025,4]]},"reference":[{"key":"10.32074\/1591-951X-1117_ref1","doi-asserted-by":"crossref","unstructured":"Valle JW, Kelley RK, Nervi B, et al. Biliary tract cancer. The Lancet. 2021;397:428-444. https:\/\/doi.org\/10.1016\/S0140-6736(21)00153-7.","DOI":"10.1016\/S0140-6736(21)00153-7"},{"key":"10.32074\/1591-951X-1117_ref2","doi-asserted-by":"crossref","unstructured":"Banales JM, Cardinale V, Carpino G, et al. Cholangiocarcinoma: current knowledge and future perspectives consensus statement from the European Network for the Study of Cholangiocarcinoma (ENS-CCA). Nat Rev Gastroenterol Hepatol. 2016;13:261-280. https:\/\/doi.org\/10.1038\/nrgastro.2016.51.","DOI":"10.1038\/nrgastro.2016.51"},{"key":"10.32074\/1591-951X-1117_ref3","doi-asserted-by":"crossref","unstructured":"Ludwig DR, Anderson MA, Itani M, et al. Secondary sclerosing cholangitis: mimics of primary sclerosing cholangitis. Abdom Radiol NY. 2023;48:151-165. https:\/\/doi.org\/10.1007\/s00261-022-03551-z","DOI":"10.1007\/s00261-022-03551-z"},{"key":"10.32074\/1591-951X-1117_ref4","doi-asserted-by":"crossref","unstructured":"Chapman RW, Williamson KD. Are Dominant Strictures in Primary Sclerosing Cholangitis a Risk Factor for Cholangiocarcinoma? Curr Hepatol Rep. 2017;16:124-129. https:\/\/doi.org\/10.1007\/s11901-017-0341-2","DOI":"10.1007\/s11901-017-0341-2"},{"key":"10.32074\/1591-951X-1117_ref5","doi-asserted-by":"crossref","unstructured":"Dumonceau JM, Delhaye M, Charette N, Farina A. Challenging biliary strictures: pathophysiological features, differential diagnosis, diagnostic algorithms, and new clinically relevant biomarkers -part 1. Ther Adv Gastroenterol. 2020;13:1756284820927292. https:\/\/doi.org\/10.1177\/1756284820927292","DOI":"10.1177\/1756284820927292"},{"key":"10.32074\/1591-951X-1117_ref6","doi-asserted-by":"crossref","unstructured":"Taghavi SA, Eshraghian A, Niknam R, et al. Diagnosis of cholangiocarcinoma in primary sclerosing cholangitis. Expert Rev Gastroenterol Hepatol. 2018;12:575-584. https:\/\/doi.org\/10.1080\/17474124.2018.1473761.","DOI":"10.1080\/17474124.2018.1473761"},{"key":"10.32074\/1591-951X-1117_ref7","doi-asserted-by":"crossref","unstructured":"Ahlawat S, Al-Kawas FH. ERCP in the evaluation of biliary strictures: Tissue is the issue! Is FISH the answer? Gastrointest Endosc. 2022;95:893-895. https:\/\/doi.org\/10.1016\/j.gie.2021.12.051.","DOI":"10.1016\/j.gie.2021.12.051"},{"key":"10.32074\/1591-951X-1117_ref8","doi-asserted-by":"crossref","unstructured":"Bowlus CL, Olson KA, Gershwin ME. Evaluation of indeterminate biliary strictures. Nat Rev Gastroenterol Hepatol. 2017;14(12):749. https:\/\/doi.org\/10.1038\/nrgastro.2017.154.","DOI":"10.1038\/nrgastro.2017.154"},{"key":"10.32074\/1591-951X-1117_ref9","doi-asserted-by":"crossref","unstructured":"Lindberg B, Arnelo U, Bergquist A, et al. Diagnosis of Biliary Strictures in Conjunction with Endoscopic Retrograde Cholangiopancreaticography, with Special Reference to Patients with Primary Sclerosing Cholangitis. Endoscopy. 2002;34:909-916. https:\/\/doi.org\/10.1055\/s-2002-35298.","DOI":"10.1055\/s-2002-35298"},{"key":"10.32074\/1591-951X-1117_ref10","doi-asserted-by":"crossref","unstructured":"Singhi AD, Nikiforova MN, Chennat J, et al. Integrating next-generation sequencing to endoscopic retrograde cholangiopancreatography (ERCP)-obtained biliary specimens improves the detection and management of patients with malignant bile duct strictures. Gut. 2020;69:52-61. https:\/\/doi.org\/10.1136\/gutjnl-2018-317817.","DOI":"10.1136\/gutjnl-2018-317817"},{"key":"10.32074\/1591-951X-1117_ref11","doi-asserted-by":"crossref","unstructured":"Nguyen CH, Harada K. Adult bile duct strictures: differentiating benign biliary stenosis from cholangiocarcinoma. Med Mol Morphol. 2016;49:189-202. https:\/\/doi.org\/10.1007\/s00795-016-0143-6.","DOI":"10.1007\/s00795-016-0143-6"},{"key":"10.32074\/1591-951X-1117_ref12","doi-asserted-by":"crossref","unstructured":"Salomao M, Gonda TA, Margolskee E, et al. Strategies for improving diagnostic accuracy of biliary strictures. Cancer Cytopathol. 2015;123:244-252. https:\/\/doi.org\/10.1002\/cncy.21509.","DOI":"10.1002\/cncy.21509"},{"key":"10.32074\/1591-951X-1117_ref13","doi-asserted-by":"crossref","unstructured":"Adler DG, Witt B. Cytologic Diagnosis of Biliary Strictures: FISH or Cut the Sensitivity Rate? Dig Dis Sci. 2018;63:549-550. https:\/\/doi.org\/10.1007\/s10620-017-4865-7.","DOI":"10.1007\/s10620-017-4865-7"},{"key":"10.32074\/1591-951X-1117_ref14","unstructured":"Albertini E, Miranda L, Maloberti T, et al. Morphologic and molecular diagnostic criteria of malignancies in biliary strictures. Histol Histopathol. 2024 Sep 10:18811. https:\/\/doi.org\/10.14670\/HH-18-811."},{"key":"10.32074\/1591-951X-1117_ref15","doi-asserted-by":"crossref","unstructured":"Stenzinger A, Vogel A, Lehmann U, et al. Molecular profiling in cholangiocarcinoma: A practical guide to next-generation sequencing. Cancer Treat Rev. 2024;122. https:\/\/doi.org\/10.1016\/j.ctrv.2023.102649.","DOI":"10.1016\/j.ctrv.2023.102649"},{"key":"10.32074\/1591-951X-1117_ref16","doi-asserted-by":"crossref","unstructured":"Pu X, Zheng H, Yang X, Ye Q, Fan Z, Yang J, Fan X, Zhou X, Qiu Y, Huang Q, Wu H, Chen J. An assessment of chromosomal alterations detected by fluorescence in situ hybridisation in pancreatobiliary tract malignancy. BMC Gastroenterol. 2020;20:367. https:\/\/doi.org\/10.1186\/s12876-020-01439-0.","DOI":"10.1186\/s12876-020-01439-0"},{"key":"10.32074\/1591-951X-1117_ref17","doi-asserted-by":"crossref","unstructured":"Mettman D, Saeed A, Shold J, Laury R, Ly A, Khan I, Golem S, Olyaee M, O\u2019Neil M. Refined pancreatobiliary UroVysion criteria and an approach for further optimization. Cancer Med. 2021;10:5725-5738. https:\/\/doi.org\/10.1002\/cam4.4043.","DOI":"10.1002\/cam4.4043"},{"key":"10.32074\/1591-951X-1117_ref18","doi-asserted-by":"crossref","unstructured":"de Biase D, Acquaviva G, Visani M, Sanza V, Argento CM, De Leo A, Maloberti T, Pession A, Tallini G. Molecular Diagnostic of Solid Tumor Using a Next Generation Sequencing Custom-Designed Multi-Gene Panel. Diagnostics (Basel). 2020;10:250. https:\/\/doi.org\/10.3390\/diagnostics10040250.","DOI":"10.3390\/diagnostics10040250"},{"key":"10.32074\/1591-951X-1117_ref19","doi-asserted-by":"crossref","unstructured":"Fassan M, Angerilli V, Normanno N, et al. Practical guidelines for molecular testing of cholangiocarcinoma in clinical practice: Italian experts\u2019 position paper. Crit Rev Oncol Hematol. 2024;194:104224. https:\/\/doi.org\/10.1016\/j.critrevonc.2023.104224.","DOI":"10.1016\/j.critrevonc.2023.104224"},{"key":"10.32074\/1591-951X-1117_ref20","unstructured":"WHO Classification of Tumors Editorial Board. International Agency for Research on Cancer; Digestive system tumors. Lyon (France): 2019 5th ed.; vol. 1."}],"container-title":["Pathologica"],"original-title":[],"link":[{"URL":"https:\/\/www.pathologica.it\/article\/view\/1196","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,10]],"date-time":"2025-04-10T07:39:44Z","timestamp":1744270784000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.pathologica.it\/article\/view\/1196"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4]]},"references-count":20,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,2]]}},"URL":"https:\/\/doi.org\/10.32074\/1591-951x-1117","relation":{},"ISSN":["1591-951X"],"issn-type":[{"value":"1591-951X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4]]},"article-number":"3"}}