{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T19:01:15Z","timestamp":1777748475773,"version":"3.51.4"},"reference-count":33,"publisher":"SAGE Publications","issue":"1-2","license":[{"start":{"date-parts":[[2015,7,3]],"date-time":"2015-07-03T00:00:00Z","timestamp":1435881600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["In Silico Biology"],"published-print":{"date-parts":[[2015,7,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    We develop an exact and flexible mathematical model for Lutz and Bujard\u2019s controllable promoters. It can be used as a building block for modeling genetic systems based on them. Special attention is paid to deduce all the model parameters from reported (\n                    <jats:italic>in vitro<\/jats:italic>\n                    ) experimental data. We validate our model by comparing the regulatory ranges measured\n                    <jats:italic>in vivo<\/jats:italic>\n                    by Lutz and Bujard against the ranges predicted by the model, and which are calculated as the reporter activity obtained under inducing conditions divided by the activity measured under maximal repression. In particular, we verify Bond et\u00a0al. assertion that the cooperativity between two\n                    <jats:italic>lac<\/jats:italic>\n                    operators can be assumed to be negligible when their central base pairs are separated by 22 or 32 bp [Gene repression by minimal\n                    <jats:italic>lac<\/jats:italic>\n                    loops\n                    <jats:italic>in vivo<\/jats:italic>\n                    , Nucleic Acids Res,\n                    <jats:bold>38<\/jats:bold>\n                    (2010) 8072\u20138082]. Moreover, we also find that the probability that two repressors\n                    <jats:italic>LacI<\/jats:italic>\n                    bind to these operators at the same time can be assumed to be negligible as well. We finally use the model for the promoter P\n                    <jats:sub>\n                      <jats:italic>LlacO<\/jats:italic>\n                      -1\n                    <\/jats:sub>\n                    to analyze a synthetic genetic oscillator recently build by Stricker et\u00a0al. [A fast, robust and tunable synthetic gene oscillator, Nature,\n                    <jats:bold>456<\/jats:bold>\n                    (2008) 516\u2013519].\n                  <\/jats:p>","DOI":"10.3233\/isb-150465","type":"journal-article","created":{"date-parts":[[2015,7,10]],"date-time":"2015-07-10T11:08:09Z","timestamp":1436526489000},"page":"69-82","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["A simple model for Lutz and Bujard\u2019s controllable promoters and its application for analyzing a simple genetic oscillator"],"prefix":"10.1177","volume":"12","author":[{"given":"CG","family":"Zamora-Chimal","sequence":"first","affiliation":[{"name":"Centro de Investigaci\u00f3n y de Estudios Avanzados del IPN, Unidad Monterrey, Parque de Investigaci\u00f3n e Innovaci\u00f3n Tecnol\u00f3gica, Apodaca NL, M\u00e9xico"}]},{"given":"ES","family":"Zeron","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n y de Estudios Avanzados del IPN, Departamento de Matem\u00e1ticas, Av. Instituto Polit\u00e9cnico Nacional M\u00e9xico DF, M\u00e9xico"}]}],"member":"179","published-online":{"date-parts":[[2015,7,3]]},"reference":[{"key":"e_1_3_1_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.6.1203"},{"key":"e_1_3_1_3_1","unstructured":"Expressys: Tools for gene expression http:\/\/www.expressys.com"},{"key":"e_1_3_1_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/35002125"},{"key":"e_1_3_1_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature07389"},{"key":"e_1_3_1_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10529-009-0058-6"},{"key":"e_1_3_1_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-011-3530-x"},{"key":"e_1_3_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2014.02.008"},{"key":"e_1_3_1_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1990.tb08199.x"},{"key":"e_1_3_1_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1994.tb06637.x"},{"key":"e_1_3_1_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkq755"},{"key":"e_1_3_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.crvi.2005.04.004"},{"key":"e_1_3_1_13_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1996.0143"},{"key":"e_1_3_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(83)80166-1"},{"key":"e_1_3_1_15_1","unstructured":"Uniprot.org: Tetracycline repressor protein TetR http:\/\/www.uniprot.org\/uniprot\/Q79VX4"},{"key":"e_1_3_1_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.12.3019-3025.1998"},{"key":"e_1_3_1_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00679a024"},{"key":"e_1_3_1_18_1","doi-asserted-by":"publisher","DOI":"10.2307\/1990339"},{"key":"e_1_3_1_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/j100540a008"},{"key":"e_1_3_1_20_1","doi-asserted-by":"publisher","DOI":"10.1063\/1.2710253"},{"key":"e_1_3_1_21_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.56.6.1891"},{"key":"e_1_3_1_22_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.59.4.1259"},{"key":"e_1_3_1_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(70)90074-4"},{"key":"e_1_3_1_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.23.8973"},{"key":"e_1_3_1_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(85)90250-8"},{"key":"e_1_3_1_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(84)90262-6"},{"key":"e_1_3_1_27_1","unstructured":"BremerHDennisPP1996Modulation of chemical composition and other parameters of the cell by growth rateNeidhardtFCEscherichia coli and Salmonella: Cellular and molecular biology2nd Edition15531569ASM pressWashington D.C"},{"key":"e_1_3_1_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1987.tb02624.x"},{"key":"e_1_3_1_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)43453-3"},{"key":"e_1_3_1_30_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.77.10.5634"},{"key":"e_1_3_1_31_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0023126"},{"key":"e_1_3_1_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.260380702"},{"key":"e_1_3_1_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)70435-9"},{"key":"e_1_3_1_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)63147-3"}],"container-title":["In Silico Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.3233\/ISB-150465","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.3233\/ISB-150465","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.3233\/ISB-150465","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T11:49:22Z","timestamp":1777463362000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.3233\/ISB-150465"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,7,3]]},"references-count":33,"journal-issue":{"issue":"1-2","published-print":{"date-parts":[[2015,7,3]]}},"alternative-id":["10.3233\/ISB-150465"],"URL":"https:\/\/doi.org\/10.3233\/isb-150465","relation":{},"ISSN":["1386-6338","1434-3207"],"issn-type":[{"value":"1386-6338","type":"print"},{"value":"1434-3207","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,7,3]]}}}