{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T19:00:43Z","timestamp":1777748443636,"version":"3.51.4"},"reference-count":0,"publisher":"SAGE Publications","issue":"5-6","license":[{"start":{"date-parts":[[2010,11,1]],"date-time":"2010-11-01T00:00:00Z","timestamp":1288569600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["In Silico Biology"],"published-print":{"date-parts":[[2010,11]]},"abstract":"<jats:p>A major problem in designing vaccine for the dengue virus has been\n\t\t\t the high antigenic variability in the envelope protein of different virus\n\t\t\t strains. In this study, a computational approach was adopted to identify a\n\t\t\t multi-epitope vaccine candidate against dengue virus that may be suitable for\n\t\t\t large populations in the dengue-endemic regions. Different bioinformatics tools\n\t\t\t were exploited that helped the identification of a conserved immunological\n\t\t\t hot-spot in the dengue envelope protein. The tools also rendered the prediction\n\t\t\t of immunogenicity and population coverage to the proposed 'in silico' vaccine\n\t\t\t candidate against dengue. A peptide region, spanning 19 amino acids, was\n\t\t\t identified in the envelope protein which found to be conserved in all four\n\t\t\t types of dengue viruses. Ten proteasomal cleavage sites were identified within\n\t\t\t the 19-mer conserved peptide sequence and a total of 8 overlapping putative\n\t\t\t cytotoxic T cell (CTL) epitopes were identified. The immunogenicity of these\n\t\t\t epitopes was evaluated in terms of their binding affinities to and dissociation\n\t\t\t half-time from respective human leukocyte antigen (HLA) molecules. The HLA\n\t\t\t allele frequencies were studied among populations in the dengue endemic regions\n\t\t\t and compared with respect to HLA restriction patterns of the overlapping\n\t\t\t epitopes. The cumulative population coverage for these epitopes as vaccine\n\t\t\t candidates was high ranging from approximately 80% to 92%. Structural analysis\n\t\t\t suggested that a 9-mer epitope fitted well into the peptide-binding groove of\n\t\t\t HLA-A*0201. In conclusion, the 19-mer epitope cluster was shown to have the\n\t\t\t potential for use as a vaccine candidate against dengue.<\/jats:p>","DOI":"10.3233\/isb-2010-0435","type":"journal-article","created":{"date-parts":[[2019,12,2]],"date-time":"2019-12-02T22:48:10Z","timestamp":1575326890000},"page":"235-246","source":"Crossref","is-referenced-by-count":47,"title":["A Computational Approach for Identification of Epitopes in Dengue\t\t\t Virus Envelope Protein: A Step Towards Designing a Universal Dengue Vaccine\t\t\t Targeting Endemic Regions"],"prefix":"10.1177","volume":"10","author":[{"given":"Sajib","family":"Chakraborty","sequence":"first","affiliation":[{"name":"Bioinformatics and Stress Biology Unit, Department of\r\t\t\t Biochemistry and Molecular Biology, University of Dhaka, Dhaka,\r\t\t\t Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rajib","family":"Chakravorty","sequence":"additional","affiliation":[{"name":"Victoria Research Laboratory, Department of Electrical\r\t\t\t and Electronic Engineering National ICT Australia (NICTA), Victoria,\r\t\t\t Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Musaddeque","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Bioinformatics and Stress Biology Unit, Department of\r\t\t\t Biochemistry and Molecular Biology, University of Dhaka, Dhaka,\r\t\t\t Bangladesh"},{"name":"Department of Biological Sciences, Brock University,\r\t\t\t Ontario, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Atiqur","family":"Rahman","sequence":"additional","affiliation":[{"name":"Bioinformatics and Stress Biology Unit, Department of\r\t\t\t Biochemistry and Molecular Biology, University of Dhaka, Dhaka,\r\t\t\t Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"TM Zaved","family":"Waise","sequence":"additional","affiliation":[{"name":"Laboratory Sciences Division, International Centre for\r\t\t\t Diarrhoeal Disease Research Bangladesh (ICDDR, B), Mohakhali, Dhaka,\r\t\t\t Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Faizule","family":"Hassan","sequence":"additional","affiliation":[{"name":"Laboratory Sciences Division, International Centre for\r\t\t\t Diarrhoeal Disease Research Bangladesh (ICDDR, B), Mohakhali, Dhaka,\r\t\t\t Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mustafizur","family":"Rahman","sequence":"additional","affiliation":[{"name":"Bioinformatics and Stress Biology Unit, Department of\r\t\t\t Biochemistry and Molecular Biology, University of Dhaka, Dhaka,\r\t\t\t Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sohel","family":"Shamsuzzaman","sequence":"additional","affiliation":[{"name":"Bioinformatics and Stress Biology Unit, Department of\r\t\t\t Biochemistry and Molecular Biology, University of Dhaka, Dhaka,\r\t\t\t Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2010,11]]},"container-title":["In Silico Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.3233\/ISB-2010-0435","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.3233\/ISB-2010-0435","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T11:49:16Z","timestamp":1777463356000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.3233\/ISB-2010-0435"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,11]]},"references-count":0,"journal-issue":{"issue":"5-6","published-print":{"date-parts":[[2010,11]]}},"alternative-id":["10.3233\/ISB-2010-0435"],"URL":"https:\/\/doi.org\/10.3233\/isb-2010-0435","relation":{"is-cited-by":[{"id-type":"doi","id":"10.3389\/fphar.2016.00153","asserted-by":"object"},{"id-type":"doi","id":"10.1155\/2018\/6718083","asserted-by":"object"},{"id-type":"doi","id":"10.3389\/fchem.2018.00246","asserted-by":"object"}]},"ISSN":["1386-6338","1434-3207"],"issn-type":[{"value":"1386-6338","type":"print"},{"value":"1434-3207","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,11]]}}}