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At the same time, microbes degrade the residual oil and change the wettability of pore wall. Consequently, the displacement exhibits a decrease of 51.86% in membrane residual oil and an increase of 17.44% in recovery compared with water flooding. The Geobacillus stearothermophilus producing bio-emulsifier and its metabolic products can effectively emulsify reservoir crude oil and reduce the oil-water interfacial tension. In the end and the formed emulsion featured high viscosity can improve the oil flow rate and expanded wave volume of injected fluid. Correspondingly, the cluster-like, columnar residual oil were reduced by 64%, 68% respectively. In the micro porous test cell, the in-situ cultured microbes rely on specific life activities (interfacial tropism and in situ metabolism, etc.) to strip the residual oil off the wall of orifice deep inside the blind-ends, which cannot be achieved by the exogenous injection method. After the in-situ microbial culture process, the residual oil in deep blind-ends was reduced by about 47%, and the recovered oil was increased about 15% compared with exogenous injection method.<\/jats:p>","DOI":"10.3233\/jcm-237072","type":"journal-article","created":{"date-parts":[[2024,3,8]],"date-time":"2024-03-08T10:19:08Z","timestamp":1709893148000},"page":"463-472","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Mechanism of action of\n                    <i>Geobacillus stearothermophilus<\/i>\n                    on different shapes of residual oil"],"prefix":"10.66113","volume":"24","author":[{"given":"Hongyan","family":"Han","sequence":"first","affiliation":[{"name":"Hebei Vocational University of Industry and Technology","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruiying","family":"Shi","sequence":"additional","affiliation":[{"name":"Hebei Vocational University of Industry and Technology","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yaoyao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Hebei Vocational University of Industry and Technology","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Na","family":"Li","sequence":"additional","affiliation":[{"name":"Hebei University of Science and Technology","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linghe","family":"Fan","sequence":"additional","affiliation":[{"name":"Hebei Vocational University of Industry and Technology","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Research Institute of Petroleum Engineering Technology, Shengli Oilfield Branch Company, SINOPEC, Dongying, Shandong, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"55691","published-online":{"date-parts":[[2024,3]]},"reference":[{"key":"e_1_3_4_2_2","first-page":"126","article-title":"Research on remaining oil distribution regular with the application of facies controlled modeling and reservoir numerical simulation","volume":"616","author":"Wei HB","year":"2013","unstructured":"WeiHB ShiSM ZhaoP, et al. 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