{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,1,26]],"date-time":"2024-01-26T00:12:38Z","timestamp":1706227958688},"reference-count":0,"publisher":"IOS Press","isbn-type":[{"value":"9781643684567","type":"print"},{"value":"9781643684574","type":"electronic"}],"license":[{"start":{"date-parts":[[2024,1,25]],"date-time":"2024-01-25T00:00:00Z","timestamp":1706140800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,1,25]]},"abstract":"<jats:p>With the advancement of genomic engineering and genetic modification techniques, the uptake of computational tools to design guide RNA increased drastically. Searching for genomic targets to design guides with maximum on-target activity (efficiency) and minimum off-target activity (specificity) is now an essential part of genome editing experiments. Today, a variety of tools exist that allow the search of genomic targets and let users customize their search parameters to better suit their experiments. Here we present an overview of different ways to visualize these searched CRISPR target sites along with specific downstream information like primer design, restriction enzyme activity and mutational outcome prediction after a double-stranded break. We discuss the importance of a good visualization summary to interpret information along with different ways to represent similar information effectively.<\/jats:p>","DOI":"10.3233\/shti231069","type":"book-chapter","created":{"date-parts":[[2024,1,25]],"date-time":"2024-01-25T10:25:07Z","timestamp":1706178307000},"source":"Crossref","is-referenced-by-count":0,"title":["Data Visualization of CRISPR-Cas9 Guide RNA Design Tools"],"prefix":"10.3233","author":[{"given":"Yatish","family":"Jain","sequence":"first","affiliation":[{"name":"Australian e-Health Research Centre, Commonwealth Scientific and Industrial Research Organisation, New South Wales, Sydney, Australia"},{"name":"Department of Biomedical Sciences, Macquarie University, New South Wales, Sydney, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fathima Afra Mohamed","family":"Izzath","sequence":"additional","affiliation":[{"name":"School of Science, RMIT University, Melbourne 3000, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laurence O.W.","family":"Wilson","sequence":"additional","affiliation":[{"name":"Australian e-Health Research Centre, Commonwealth Scientific and Industrial Research Organisation, New South Wales, Sydney, Australia"},{"name":"Department of Biomedical Sciences, Macquarie University, New South Wales, Sydney, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Denis C.","family":"Bauer","sequence":"additional","affiliation":[{"name":"Australian e-Health Research Centre, Commonwealth Scientific and Industrial Research Organisation, New South Wales, Sydney, Australia"},{"name":"Department of Biomedical Sciences, Macquarie University, New South Wales, Sydney, Australia"},{"name":"Applied BioSciences, Faculty of Science and Engineering, Macquarie University, New South Wales, Sydney, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"7437","container-title":["Studies in Health Technology and Informatics","MEDINFO 2023 \u2014 The Future Is Accessible"],"original-title":[],"link":[{"URL":"https:\/\/ebooks.iospress.nl\/pdf\/doi\/10.3233\/SHTI231069","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,25]],"date-time":"2024-01-25T10:25:08Z","timestamp":1706178308000},"score":1,"resource":{"primary":{"URL":"https:\/\/ebooks.iospress.nl\/doi\/10.3233\/SHTI231069"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1,25]]},"ISBN":["9781643684567","9781643684574"],"references-count":0,"URL":"https:\/\/doi.org\/10.3233\/shti231069","relation":{},"ISSN":["0926-9630","1879-8365"],"issn-type":[{"value":"0926-9630","type":"print"},{"value":"1879-8365","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1,25]]}}}