{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T12:04:40Z","timestamp":1777464280178,"version":"3.51.4"},"reference-count":28,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2015,4,30]],"date-time":"2015-04-30T00:00:00Z","timestamp":1430352000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Bladder Cancer"],"published-print":{"date-parts":[[2015,4,30]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>A second primary tumors of the urethra (urethral recurrence) after radical cystectomy has been reported to be more infrequent in patients with ileal orthotopic (neobladder) compared to incontinent diversions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>To investigate whether an altered immunogenic environment of urethral tissue is induced by urethro-ileal anastomosis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>Between 10\/2008 and 12\/2009 urethral biopsies of 19 patients (9 neobladder patients, 10 control patients without urethra-ileal anastomosis) were evaluated by conventional histopathological examination and immunohistochemistry for T- (CD3\/CD5, CD4, CD8) and B-cell markers (CD20\/22, CD79a, CD138). After semi-quantitative assessment, relative cell fractions (B vs. T cells) and subclasses (T4-helper vs. T8-killer cells vs. B cell clones, plasma cells) in neobladder vs. control patients were studied. Unpaired t-test was used for statistical analysis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>Of 19 included patients, 16 were eligible for analysis (7x neobladder, 9x controls). All neobladder patients had undergone cystectomy for UBC. Comparing relative fractions of cells positive for T- and B-cell markers in NB and CO patients, no statistical differences were observed. In 4\/7 neobladder patients relative fraction of CD79a positive B-cells was higher than relative fraction of CD3\/CD5 positive T-cells (B\/T-ratio 1.3). B cells were predominantly CD138 positive plasma cells (5\/7 NB patients). Relative B-cell fraction was lower than T-cell fraction in 7\/9 control patients (B\/T-ratio 0.6). Neither CD 138 positive plasma cells nor CD22 positive B cell clones were predominant. T-helper and CD8 positive T-killer cells were equally distributed in both neobladder (CD4\/CD8-ratio: 2.1) and control patients (CD4\/CD8-ratio: 1.9).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>Comparing neobadder and control patients the distribution of B- and T-cells was statistically not different. However, a trend towards an increased presence of B-cells in urethral tissues of NB patients that could become relevant in a larger study might be suggestive for an immunological response induced by connecting urethral and ileal tissue.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3233\/blc-140001","type":"journal-article","created":{"date-parts":[[2015,5,29]],"date-time":"2015-05-29T05:13:45Z","timestamp":1432876425000},"page":"97-103","source":"Crossref","is-referenced-by-count":4,"title":["Do Orthotopic Ileal Diversions Induce Immunological Changes in Retained Urethral Tissue?"],"prefix":"10.1177","volume":"1","author":[{"given":"G\u00fcnter","family":"Niegisch","sequence":"first","affiliation":[{"name":"Department of Urology, Radboud University Medical Center, Nijmegen, The Netherlands"},{"name":"Department of Urology, Medical Faculty, Heinrich-Heine-University, D\u00fcsseldorf, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christina","family":"Hulsbergen - van de Kaa","sequence":"additional","affiliation":[{"name":"Department of Pathology, Radboud University Medical Center, Nijmegen, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martine","family":"Ploeg","sequence":"additional","affiliation":[{"name":"Department of Urology, Radboud University Medical Center, Nijmegen, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kees","family":"Hendricksen","sequence":"additional","affiliation":[{"name":"Department of Urology, Radboud University Medical Center, Nijmegen, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. Alfred","family":"Witjes","sequence":"additional","affiliation":[{"name":"Department of Urology, Radboud University Medical Center, Nijmegen, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2015,4,30]]},"reference":[{"key":"r1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2011.08.030"},{"key":"r2","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2007.06.045"},{"key":"r3","doi-asserted-by":"publisher","DOI":"10.1097\/01.ju.0000149679.56884.0f"},{"key":"r4","doi-asserted-by":"publisher","DOI":"10.1007\/s00345-012-0905-2"},{"key":"r5","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2004.06.012"},{"key":"r6","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(05)64062-1"},{"key":"r7","doi-asserted-by":"publisher","DOI":"10.1097\/01.ju.0000135626.91587.c8"},{"key":"r8","doi-asserted-by":"publisher","DOI":"10.1097\/01.ju.0000138616.05218.21"},{"key":"r9","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(01)65462-4"},{"key":"r10","doi-asserted-by":"publisher","DOI":"10.1038\/nrurol.2014.15"},{"key":"r11","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2013.02.002"},{"key":"r12","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2009.09095.x"},{"key":"r13","doi-asserted-by":"publisher","DOI":"10.1177\/030089160809400510"},{"key":"r14","doi-asserted-by":"publisher","DOI":"10.1007\/BF01076949"},{"key":"r15","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0069892"},{"key":"r16","doi-asserted-by":"publisher","DOI":"10.1111\/j.1442-2042.1997.tb00143.x"},{"key":"r17","doi-asserted-by":"publisher","DOI":"10.1007\/BF00262987"},{"key":"r18","doi-asserted-by":"publisher","DOI":"10.1046\/j.1464-410X.1996.01928.x"},{"key":"r19","doi-asserted-by":"publisher","DOI":"10.1046\/j.1464-410X.2001.02394.x"},{"key":"r20","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2014.01.008"},{"key":"r21","doi-asserted-by":"publisher","DOI":"10.4161\/onci.25443"},{"key":"r22","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2012.347"},{"key":"r23","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2012.211"},{"key":"r24","doi-asserted-by":"publisher","DOI":"10.1111\/cas.12075"},{"key":"r25","doi-asserted-by":"publisher","DOI":"10.3325\/cmj.2012.53.598"},{"key":"r26","doi-asserted-by":"publisher","DOI":"10.1016\/0959-8049(93)90579-5"},{"key":"r27","doi-asserted-by":"publisher","DOI":"10.1007\/BF01771456"},{"key":"r28","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.1990.tb14751.x"}],"container-title":["Bladder Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.3233\/BLC-140001","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.3233\/BLC-140001","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.3233\/BLC-140001","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T13:22:50Z","timestamp":1777382570000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.3233\/BLC-140001"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,4,30]]},"references-count":28,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2015,4,30]]}},"alternative-id":["10.3233\/BLC-140001"],"URL":"https:\/\/doi.org\/10.3233\/blc-140001","relation":{},"ISSN":["2352-3727","2352-3735"],"issn-type":[{"value":"2352-3727","type":"print"},{"value":"2352-3735","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,4,30]]}}}