{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T19:12:05Z","timestamp":1789672325014,"version":"4.0.1"},"reference-count":0,"publisher":"SAGE Publications","issue":"1_suppl","license":[{"start":{"date-parts":[[2015,2,1]],"date-time":"2015-02-01T00:00:00Z","timestamp":1422748800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2015,2,1]],"date-time":"2015-02-01T00:00:00Z","timestamp":1422748800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Bio-Medical Materials and Engineering"],"published-print":{"date-parts":[[2015,2]]},"abstract":"<jats:label>Abstract<\/jats:label>\n                  <jats:p>A surface plasmon resonance (SPR)-based biosensor was developed for specific detection of nine common respiratory virus, including influenza A and influenza B, H1N1, respiratory syncytial virus (RSV), parainfluenza virus 1-3 (PIV1, 2, 3), adenovirus, and severe acute respiratory syndrome coronavirus (SARS). The SPR biosensor was developed by immobilizing nine respiratory virus-specific oligonucleotides in an SPR chip. To increase the biosensor sensitivity, biotin was used to label the PCR primer and further amplify the signal by introducing streptavidin after hybridization. Throat swab specimens representing nine common respiratory viruses were tested by the innovative SPR-based biosensor to evaluate the sensitivity, specificity and reproducibility of this method. Results suggest that this biosensor has the potential to simultaneously identify common respiratory viruses.<\/jats:p>","DOI":"10.3233\/bme-151526","type":"journal-article","created":{"date-parts":[[2015,8,17]],"date-time":"2015-08-17T15:52:23Z","timestamp":1439826743000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":37,"title":["Development of SPR biosensor for simultaneous detection of multiplex respiratory viruses"],"prefix":"10.1177","volume":"26","author":[{"given":"Lei","family":"Shi","sequence":"first","affiliation":[{"name":"Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Antibody Engineering Center of Jinan University, Guangzhou, 510632, P.R. China"},{"name":"Shenzhen Academy of Inspection and Quarantine, Shenzhen, 518010, P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiuxiang","family":"Sun","sequence":"additional","affiliation":[{"name":"Feng Gang Hospital, Dongguan, 523695, P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian\u2019an","family":"He","sequence":"additional","affiliation":[{"name":"Central Laboratory of Health Quarantine, Shenzhen International Travel Health Care Center, Shenzhen Entry-exit Inspection and Quarantine Bureau, Shenzhen, 518033, P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"Xu","sequence":"additional","affiliation":[{"name":"Central Laboratory of Health Quarantine, Shenzhen International Travel Health Care Center, Shenzhen Entry-exit Inspection and Quarantine Bureau, Shenzhen, 518033, P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunxiao","family":"Liu","sequence":"additional","affiliation":[{"name":"Central Laboratory of Health Quarantine, Shenzhen International Travel Health Care Center, Shenzhen Entry-exit Inspection and Quarantine Bureau, Shenzhen, 518033, P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunzhong","family":"Zhao","sequence":"additional","affiliation":[{"name":"Central Laboratory of Health Quarantine, Shenzhen International Travel Health Care Center, Shenzhen Entry-exit Inspection and Quarantine Bureau, Shenzhen, 518033, P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunqing","family":"Xu","sequence":"additional","affiliation":[{"name":"Central Laboratory of Health Quarantine, Shenzhen International Travel Health Care Center, Shenzhen Entry-exit Inspection and Quarantine Bureau, Shenzhen, 518033, P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changlin","family":"Wu","sequence":"additional","affiliation":[{"name":"Central Laboratory of Health Quarantine, Shenzhen International Travel Health Care Center, Shenzhen Entry-exit Inspection and Quarantine Bureau, Shenzhen, 518033, P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junjian","family":"Xiang","sequence":"additional","affiliation":[{"name":"Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Antibody Engineering Center of Jinan University, Guangzhou, 510632, P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dayong","family":"Gu","sequence":"additional","affiliation":[{"name":"Central Laboratory of Health Quarantine, Shenzhen International Travel Health Care Center, Shenzhen Entry-exit Inspection and Quarantine Bureau, Shenzhen, 518033, P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Long","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Zhujiang Hospital, Southern Medical University, Guangzhou, 510282, P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hekui","family":"Lan","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Zhujiang Hospital, Southern Medical University, Guangzhou, 510282, P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2015,2]]},"container-title":["Bio-Medical Materials and Engineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.3233\/BME-151526","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.3233\/BME-151526","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T13:25:46Z","timestamp":1777382746000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.3233\/BME-151526"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,2]]},"references-count":0,"journal-issue":{"issue":"1_suppl","published-print":{"date-parts":[[2015,2]]}},"alternative-id":["10.3233\/BME-151526"],"URL":"https:\/\/doi.org\/10.3233\/bme-151526","relation":{},"ISSN":["0959-2989","1878-3619"],"issn-type":[{"value":"0959-2989","type":"print"},{"value":"1878-3619","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,2]]}}}