{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T08:40:40Z","timestamp":1789461640336,"version":"build-2803163510"},"reference-count":11,"publisher":"SAGE Publications","issue":"1_suppl","license":[{"start":{"date-parts":[[2017,1,1]],"date-time":"2017-01-01T00:00:00Z","timestamp":1483228800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Bio-Medical Materials and Engineering"],"published-print":{"date-parts":[[2017,1]]},"abstract":"<jats:p>\n                    Wharton\u2019s jelly mesenchymal stem cells (WJ-MSCs) are widely used in tissue engineering. In vascular engineering, the ability to obtain a vessel replacement with contractile smooth muscle cells (SMC) is a key factor. In this work, we demonstrated that WJ-MSCs differentiate towards a SMC phenotype with various stimulations\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    and that the modification of redox state could be involved. WJ-MSCs were isolated from umbilical cords. After their expansion, the cells were stimulated with ascorbic acid (AA, 300\n                    <jats:italic toggle=\"yes\">\u03bc<\/jats:italic>\n                    M) or transforming growth factor (TGF)-\n                    <jats:inline-formula>\n                      <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" overflow=\"scroll\">\n                        <mml:mi mathvariant=\"italic\">\u03b2<\/mml:mi>\n                        <mml:mn>1<\/mml:mn>\n                      <\/mml:math>\n                    <\/jats:inline-formula>\n                    (1 to 5 ng\/mL). SMC markers were analyzed by Western blot. Modification of redox state was evaluated by reactive oxygen species (ROS) production and glutathione (GSH) content measurements. TGF-\n                    <jats:inline-formula>\n                      <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" overflow=\"scroll\">\n                        <mml:mi mathvariant=\"italic\">\u03b2<\/mml:mi>\n                        <mml:mn>1<\/mml:mn>\n                      <\/mml:math>\n                    <\/jats:inline-formula>\n                    or AA-stimulated WJ-MSCs express early and intermediate SMC markers. TGF-\n                    <jats:inline-formula>\n                      <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" overflow=\"scroll\">\n                        <mml:mi mathvariant=\"italic\">\u03b2<\/mml:mi>\n                        <mml:mn>1<\/mml:mn>\n                      <\/mml:math>\n                    <\/jats:inline-formula>\n                    (5 ng\/mL) modifies the redox state by a ROS production and a GSH content drop, while AA has no effect. This work showed that TGF-\n                    <jats:inline-formula>\n                      <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" overflow=\"scroll\">\n                        <mml:mi mathvariant=\"italic\">\u03b2<\/mml:mi>\n                        <mml:mn>1<\/mml:mn>\n                      <\/mml:math>\n                    <\/jats:inline-formula>\n                    and AA are effective SMC phenotype inducers to differentiate WJ-MSCs.\n                  <\/jats:p>","DOI":"10.3233\/bme-171630","type":"journal-article","created":{"date-parts":[[2017,3,31]],"date-time":"2017-03-31T18:12:08Z","timestamp":1490983928000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":5,"title":["Transforming growth factor-beta 1 or ascorbic acid are able to differentiate Wharton\u2019s jelly mesenchymal stem cells towards a smooth muscle phenotype"],"prefix":"10.1177","volume":"28","author":[{"given":"B.","family":"Mesure","sequence":"first","affiliation":[{"name":"CNRS-Universit\u00e9 de Lorraine","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Huber-Villaume","sequence":"additional","affiliation":[{"name":"CNRS-Universit\u00e9 de Lorraine","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Menu","sequence":"additional","affiliation":[{"name":"CNRS-Universit\u00e9 de Lorraine","place":["France"]},{"name":"Universit\u00e9 de Lorraine","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c9.","family":"Velot","sequence":"additional","affiliation":[{"name":"CNRS-Universit\u00e9 de Lorraine","place":["France"]},{"name":"Universit\u00e9 de Lorraine","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2017,1]]},"reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2016.04.004"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1089\/ten.teb.2013.0664"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/icb.2015.94"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-008-0654-0"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.110.221135"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","unstructured":"XiaoQ.LuoZ.PepeA.E.MargaritiA.ZengL. and XuQ. Embryonic stem cell differentiation into smooth muscle cells is mediated by Nox4-produced H2O2 Am. J. Physiol. Cell Physiol.296(4) (2009) C711-23. doi:10.1152\/ajpcell.00442.2008.","DOI":"10.1152\/ajpcell.00442.2008"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2010.11.007"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.107.152736"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.3233\/BME-130754"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2015.02.053"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","unstructured":"HuT.RamachandraraoS.P.SivaS.ValanciusC.ZhuY.MahadevK.TohI.GoldsteinB.J.WoolkalisM. and SharmaK. Reactive oxygen species production via NADPH oxidase mediates TGF-beta-induced cytoskeletal alterations in endothelial cells Am. J. Physiol. Renal Physiol.289(4) (2005) F816-25. doi:10.1152\/ajprenal.00024.2005.","DOI":"10.1152\/ajprenal.00024.2005"}],"container-title":["Bio-Medical Materials and Engineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.3233\/BME-171630","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.3233\/BME-171630","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.3233\/BME-171630","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T13:26:04Z","timestamp":1777382764000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.3233\/BME-171630"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,1]]},"references-count":11,"journal-issue":{"issue":"1_suppl","published-print":{"date-parts":[[2017,1]]}},"alternative-id":["10.3233\/BME-171630"],"URL":"https:\/\/doi.org\/10.3233\/bme-171630","relation":{},"ISSN":["0959-2989","1878-3619"],"issn-type":[{"value":"0959-2989","type":"print"},{"value":"1878-3619","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,1]]}}}