{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T14:39:25Z","timestamp":1789655965396,"version":"4.0.1"},"reference-count":32,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Cancer Biomarkers"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:sec>\n                    <jats:title>BACKGROUND:<\/jats:title>\n                    <jats:p>Increasing evidence has indicated that abnormal methionine metabolic activity and tumour-associated macrophage infiltration are correlated with hepatocarcinogenesis. However, the relationship between methionine metabolic activity and tumour-associated macrophage infiltration is unclear in hepatocellular carcinoma, and it contributes to the occurrence and clinical outcome of hepatocellular carcinoma (HCC). Thus, we systematically analysed the expression patterns of methionine metabolism and macrophage infiltration in hepatocellular carcinoma using bioinformatics and machine learning methods and constructed novel diagnostic and prognostic models of HCC.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>METHODS:<\/jats:title>\n                    <jats:p>In this study, we first mined the four largest HCC mRNA microarray datasets with patient clinical data in the GEO database, including 880 tissue mRNA expression datasets. Using GSVA analysis and the CIBERSORT and EPIC algorithms, we quantified the methionine metabolic activity and macrophage infiltration degree of each sample. WGCNA was used to identify the gene modules most related to methionine metabolism and tumour-associated macrophage infiltration in HCC. The KNN algorithm was used to cluster gene expression patterns in HCC. Random forest, logistic regression, Cox regression analysis and other algorithms were used to construct the diagnosis and prognosis model of HCC. The above bioinformatics analysis results were also verified by independent datasets (TCGA-LIHC, ICGC-JP and CPTAC datasets) and immunohistochemical fluorescence based on our external HCC panel. Furthermore, we carried out pancancer analysis to verify the specificity of the above model and screened a wide range of drug candidates.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS:<\/jats:title>\n                    <jats:p>We identified two methionine metabolism and macrophage infiltration expression patterns, and their prognoses were different in hepatocellular carcinoma. We constructed novel diagnostic and prognostic models of hepatocellular carcinoma with good diagnostic efficacy and differentiation ability.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSIONS:<\/jats:title>\n                    <jats:p>Methionine metabolism is closely related to tumour-associated macrophage infiltration in hepatocellular carcinoma and can help in the clinical diagnosis and prognosis of HCC.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3233\/cbm-220421","type":"journal-article","created":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T11:51:18Z","timestamp":1690285878000},"page":"37-48","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":6,"title":["Analysis of clinical significance and molecular characteristics of methionine metabolism and macrophage-related patterns in hepatocellular carcinoma based on machine learning"],"prefix":"10.1177","volume":"39","author":[{"given":"Diguang","family":"Wen","sequence":"first","affiliation":[{"name":"Hepatobiliary Surgery Department, Second Affiliated Hospital of Chongqing Medical University, Chongqing, China"},{"name":"Department of Gastroenterology, Second Affiliated Hospital of Chongqing Medical University, Chongqing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuling","family":"Wang","sequence":"additional","affiliation":[{"name":"Hepatobiliary Surgery Department, Second Affiliated Hospital of Chongqing Medical University, Chongqing, China"},{"name":"Department of Gastroenterology, Second Affiliated Hospital of Chongqing Medical University, Chongqing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiajian","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Hepatology, Chongqing University Filing hospital, Chongqing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Hepatology, Chongqing University Filing hospital, Chongqing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zuojin","family":"Liu","sequence":"additional","affiliation":[{"name":"Hepatobiliary Surgery Department, Second Affiliated Hospital of Chongqing Medical University, Chongqing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Li","sequence":"additional","affiliation":[{"name":"Hepatobiliary Surgery Department, Second Affiliated Hospital of Chongqing Medical University, Chongqing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2024,2,1]]},"reference":[{"key":"bibr1-CBM-220421","doi-asserted-by":"publisher","DOI":"10.1038\/s41575-022-00620-y"},{"key":"bibr2-CBM-220421","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2022.02.026"},{"key":"bibr3-CBM-220421","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2022.05.018"},{"key":"bibr4-CBM-220421","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2021.10.014"},{"key":"bibr5-CBM-220421","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2014.04.035"},{"key":"bibr6-CBM-220421","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2022.02.018"},{"key":"bibr7-CBM-220421","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2019.12.015"},{"key":"bibr8-CBM-220421","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphs.2019.10.007"},{"key":"bibr9-CBM-220421","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-022-01255-w"},{"key":"bibr10-CBM-220421","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2022.04.006"},{"key":"bibr11-CBM-220421","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2022.02.030"},{"key":"bibr12-CBM-220421","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2020.10.021"},{"key":"bibr13-CBM-220421","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2022.114990"},{"key":"bibr14-CBM-220421","doi-asserted-by":"publisher","DOI":"10.1186\/s13045-022-01272-w"},{"key":"bibr15-CBM-220421","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.03.042"},{"key":"bibr16-CBM-220421","doi-asserted-by":"crossref","unstructured":"GTEx Consortium, Human genomics. The Genotype-Tissue Expression (GTEx) pilot analysis: Multitissue gene regulation in humans, Science 348 (2015), 648\u2013660.","DOI":"10.1126\/science.1262110"},{"key":"bibr17-CBM-220421","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-019-0055-9"},{"key":"bibr18-CBM-220421","doi-asserted-by":"publisher","DOI":"10.1021\/pr501254j"},{"key":"bibr19-CBM-220421","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abn4776"},{"key":"bibr20-CBM-220421","doi-asserted-by":"publisher","DOI":"10.18632\/aging.203889"},{"key":"bibr21-CBM-220421","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2021.649093"},{"key":"bibr22-CBM-220421","volume":"22","author":"Yang C.","year":"2021","journal-title":"Brief Bioinform"},{"key":"bibr23-CBM-220421","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-med-042220-021121"},{"key":"bibr24-CBM-220421","doi-asserted-by":"publisher","DOI":"10.1016\/j.crimmu.2022.05.003"},{"key":"bibr25-CBM-220421","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v29.i6.1054"},{"key":"bibr26-CBM-220421","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2023.02.033"},{"key":"bibr27-CBM-220421","doi-asserted-by":"publisher","DOI":"10.1002\/cac2.12345"},{"key":"bibr28-CBM-220421","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00047.2011"},{"key":"bibr29-CBM-220421","doi-asserted-by":"publisher","DOI":"10.2147\/JHC.S358197"},{"key":"bibr30-CBM-220421","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2023.1145408"},{"key":"bibr31-CBM-220421","doi-asserted-by":"publisher","DOI":"10.1016\/j.critrevonc.2023.103960"},{"key":"bibr32-CBM-220421","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2021.01.046"}],"container-title":["Cancer Biomarkers"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.3233\/CBM-220421","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.3233\/CBM-220421","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.3233\/CBM-220421","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T14:10:34Z","timestamp":1777385434000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.3233\/CBM-220421"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1]]},"references-count":32,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,1]]}},"alternative-id":["10.3233\/CBM-220421"],"URL":"https:\/\/doi.org\/10.3233\/cbm-220421","relation":{},"ISSN":["1574-0153","1875-8592"],"issn-type":[{"value":"1574-0153","type":"print"},{"value":"1875-8592","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1]]}}}