{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T16:38:51Z","timestamp":1777480731323,"version":"3.51.4"},"reference-count":28,"publisher":"SAGE Publications","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CBM"],"published-print":{"date-parts":[[2023,9,5]]},"abstract":"<jats:p>BACKGROUND: ALK receptor tyrosine kinase (ALK) aberrations have an established role in pathogenesis of many neoplasms, but their clinical significance in high grade serous ovarian carcinoma (HGSOC) is unclear. OBJECTIVE: To analyse the frequency of ALK overexpression, molecular abnormalities of ALK, and their impact on the progression-free survival (PFS) and overall survival (OS) in HGSOC. METHODS: Protein expression was examined by immunohistochemistry (IHC) using three different clones of anti-ALK antibody. The presence of translocations was analysed using fluorescent in situ hybridization. Next-generation sequencing was used for studying the copy number variation, as well as point mutation and translocations involving other commonly rearranged genes. RESULTS: ALK overexpression was demonstrated in up to 52% of tumours, whereas ALK copy gains in 8.2%, with no clear impact on survival. ALK point mutations were identified in 13 tumours (8.9%), with 3 belonging to the class IV showing significantly better OS. A trend suggesting better PFS was also noticed in these cases. Additionally, three gene fusions were found: ERBB2-GRB7, PRKCA-BRCA1 and SND1-BRAF, none of which has been previously described in HGSOC. CONCLUSIONS: HGSOC harbouring activating ALK mutations might be associated with a better survival, while ALK overexpression and ALK amplification does not impact the prognosis.<\/jats:p>","DOI":"10.3233\/cbm-230117","type":"journal-article","created":{"date-parts":[[2023,8,2]],"date-time":"2023-08-02T07:41:57Z","timestamp":1690962117000},"page":"17-26","source":"Crossref","is-referenced-by-count":1,"title":["Prognostic value of ALK overexpression and molecular abnormalities in high-grade serous ovarian carcinoma"],"prefix":"10.1177","volume":"38","author":[{"given":"Adam","family":"Gorczy\u0144ski","sequence":"first","affiliation":[{"name":"Department of Pathomorphology, Medical University of Gdansk, Gdansk, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kevin","family":"Miszewski","sequence":"additional","affiliation":[{"name":"Department of Urology, Medical University of Gdansk, Gdansk, 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Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agata","family":"Pospieszy\u0144ska","sequence":"additional","affiliation":[{"name":"Department of Gynecology, Obstetrics and Neonatology, Medical University of Gdansk, Gdansk, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dariusz","family":"Wydra","sequence":"additional","affiliation":[{"name":"Department of Gynecology, Obstetrics and Neonatology, Medical University of Gdansk, Gdansk, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renata","family":"Duchnowska","sequence":"additional","affiliation":[{"name":"Department of Oncology, Military Institute of Medicine, National Research Institute, Warsaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bartosz","family":"Szymanowski","sequence":"additional","affiliation":[{"name":"Department of Oncology, Military Institute of Medicine, National Research Institute, Warsaw, 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