{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T16:44:16Z","timestamp":1790181856359,"version":"4.1.0"},"reference-count":47,"publisher":"SAGE Publications","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CH"],"published-print":{"date-parts":[[2022,6,7]]},"abstract":"<jats:p>BACKGROUND: Coronavirus disease (COVID-19) associated endotheliopathy and microvascular dysfunction are of concern. OBJECTIVE: The objective of the present single-center observational pilot study was to compare endothelial glycocalyx (EG) damage and endotheliopathy in patients with severe COVID-19 (COVID-19 group) with patients with bacterial pneumonia with septic shock (non-COVID group). METHODS: Biomarkers of EG damage (syndecan-1), endothelial cells (EC) damage (thrombomodulin), and activation (P-selectin) were measured in blood on three consecutive days from admission to the intensive care unit (ICU). The sublingual microcirculation was studied by Side-stream Dark Field (SDF) imaging with automatic assessment. RESULTS: We enrolled 13 patients in the non-COVID group (mean age 70 years, 6 women), and 15 in the COVID-19 group (64 years old, 3 women). The plasma concentrations of syndecan-1 were significantly higher in the COVID-19 group during all three days. Differences regarding other biomarkers were not statistically significant. The assessment of the sublingual microcirculation showed improvement on Day 2 in the COVID-19 group. Plasma levels of C-reactive protein (CRP) were significantly higher on the first two days in the COVID-19 group. Plasma syndecan-1 and CRP were higher in patients suffering from severe COVID-19 pneumonia compared to bacterial pneumonia patients. CONCLUSIONS: These findings support the role of EG injury in the microvascular dysfunction in COVID-19 patients who require ICU.<\/jats:p>","DOI":"10.3233\/ch-221401","type":"journal-article","created":{"date-parts":[[2022,3,22]],"date-time":"2022-03-22T18:17:25Z","timestamp":1647973045000},"page":"205-219","source":"Crossref","is-referenced-by-count":18,"title":["Endothelial glycocalyx damage in patients with severe COVID-19 on mechanical ventilation \u2013 A prospective observational pilot study"],"prefix":"10.1177","volume":"81","author":[{"given":"David","family":"Astapenko","sequence":"first","affiliation":[{"name":"Department of Anesthesiology, Resuscitation and Intensive Care Medicine, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic"},{"name":"Faculty of Medicine in Hradec Kralove, Charles University, Hradec Kralove, Czech Republic"},{"name":"Center for Research and Development, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adela","family":"Tomasova","sequence":"additional","affiliation":[{"name":"Faculty of Medicine in Hradec Kralove, Charles University, Hradec Kralove, Czech Republic"},{"name":"Department of Clinical Biochemistry and Diagnostics, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alena","family":"Ticha","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry and Diagnostics, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Radomir","family":"Hyspler","sequence":"additional","affiliation":[{"name":"Faculty of Medicine in Hradec Kralove, Charles University, Hradec Kralove, Czech Republic"},{"name":"Department of Clinical Biochemistry and Diagnostics, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huey Shin","family":"Chua","sequence":"additional","affiliation":[{"name":"Faculty of Medicine in Hradec Kralove, Charles University, Hradec Kralove, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mubashar","family":"Manzoor","sequence":"additional","affiliation":[{"name":"Faculty of Medicine in Hradec Kralove, Charles University, Hradec Kralove, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roman","family":"Skulec","sequence":"additional","affiliation":[{"name":"Faculty of Medicine in Hradec Kralove, Charles University, Hradec Kralove, Czech Republic"},{"name":"Department of Anesthesiology, Perioperative Medicine, and Intensive Care, J.E. Purkinje University, Masaryk Hospital, Usti nad Labem, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Lehmann","sequence":"additional","affiliation":[{"name":"Department of Anaesthesia, Pain Management and Perioperative Medicine, Dalhousie University, Halifax, NS, Canada"},{"name":"Department of Microbiology and Immunology, Dalhousie University, Halifax, NS, Canada"},{"name":"Department of Pharmacology, Dalhousie University, Halifax, NS, Canada"},{"name":"Department of Physiology and Biophysics, Dalhousie University, Halifax, NS, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert G.","family":"Hahn","sequence":"additional","affiliation":[{"name":"Research Unit, S\u00f6dert\u00e4lje Hospital, S\u00f6dert\u00e4lje, Sweden"},{"name":"Karolinska Institutet at Danderyds Hospital (KIDS), Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manu LNG","family":"Malbrain","sequence":"additional","affiliation":[{"name":"First Department of Anaesthesia and Intensive Therapy, Medical University of Lublin, Lublin, Poland"},{"name":"International Fluid Academy, Lovenjoel, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladimir","family":"Cerny","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, Resuscitation and Intensive Care Medicine, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic"},{"name":"Faculty of Medicine in Hradec Kralove, Charles University, Hradec Kralove, Czech Republic"},{"name":"Center for Research and Development, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic"},{"name":"Department of Anesthesiology, Perioperative Medicine, and Intensive Care, J.E. 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