{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T21:17:17Z","timestamp":1777497437193,"version":"3.51.4"},"reference-count":33,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2023,6,10]],"date-time":"2023-06-10T00:00:00Z","timestamp":1686355200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Clinical Hemorheology and Microcirculation"],"published-print":{"date-parts":[[2023,9,20]]},"abstract":"<jats:p>\n                    The clinical course of essential thrombocythemia (ET) is complicated with thrombosis which significantly impacts patients\u2019 mortality. Studies have identified JAK2\n                    <jats:sup>V617F<\/jats:sup>\n                    mutation as an independent risk factor for thrombosis. Circulating extracellular vesicles (EVs) were evaluated in several studies regarding myeloproliferative neoplasms and thrombosis as potential biomarkers. The present study investigates the relationship between JAK2\n                    <jats:sup>V617F<\/jats:sup>\n                    mutation and EVs levels in 119 ET patients. Our analyses revealed that JAK2\n                    <jats:sup>V617F<\/jats:sup>\n                    -positive patients are at a significantly increased risk of thrombosis within five years before the ET diagnosis (hazard ratio [95% CI]: 11.9 [1.7\u201383.7], P\u200a=\u200a0.013), and that JAK2\n                    <jats:sup>V617F<\/jats:sup>\n                    mutation is an independent risk factor for thrombosis at ET diagnosis or during the follow-up (hazard ratio [95% CI]: 3.56 [1.47\u20138.62], P\u200a=\u200a0.005). ET patients have higher levels of platelet-EVs, erythrocyte-EVs and procoagulant activity of EVs than the healthy population. Absolute and relative counts of platelet-EVs are increased in the presence of JAK2\n                    <jats:sup>V617F<\/jats:sup>\n                    mutation ( P\u200a=\u200a0.018, P\u200a=\u200a0.024, respectively). In conclusion, our results support the role of JAK2\n                    <jats:sup>V617F<\/jats:sup>\n                    mutation in the pathogenesis of thrombosis in essential thrombocythemia through enhancing platelet activation.\n                  <\/jats:p>","DOI":"10.3233\/ch-221678","type":"journal-article","created":{"date-parts":[[2023,6,13]],"date-time":"2023-06-13T10:10:20Z","timestamp":1686651020000},"page":"359-368","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["JAK2\n                    <sup>V617F<\/sup>\n                    mutation and circulating extracellular vesicles in essential thrombocythemia"],"prefix":"10.1177","volume":"84","author":[{"given":"Mohamed Hussam","family":"Aswad","sequence":"first","affiliation":[{"name":"Department of Clinical Hematology, Faculty of Medicine, University Hospital Brno, Brno, Czech Republic"},{"name":"Faculty of Medicine, Masaryk University, Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jarmila","family":"Kissova","sequence":"additional","affiliation":[{"name":"Department of Clinical Hematology, Faculty of Medicine, University Hospital Brno, Brno, Czech Republic"},{"name":"Faculty of Medicine, Masaryk University, Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Petra","family":"Ovesna","sequence":"additional","affiliation":[{"name":"Institute of Biostatistics and Analyses, Faculty of Medicine, Masaryk University, Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucie","family":"\u0158\u00edhov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Clinical Hematology, Faculty of Medicine, University Hospital Brno, Brno, Czech Republic"},{"name":"Faculty of Medicine, Masaryk University, Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miroslav","family":"Penka","sequence":"additional","affiliation":[{"name":"Department of Clinical Hematology, Faculty of Medicine, University Hospital Brno, Brno, Czech Republic"},{"name":"Faculty of Medicine, Masaryk University, Brno, Czech 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