{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T21:17:20Z","timestamp":1777497440959,"version":"3.51.4"},"reference-count":32,"publisher":"SAGE Publications","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CH"],"published-print":{"date-parts":[[2023,10,30]]},"abstract":"<jats:p>BACKGROUND: Hydrogen is a potent antioxidant agent that can easily be administered by inhalation. The aim of the study was to evaluate whether hydrogen protects the endothelial glycocalyx layer after successful cardiopulmonary resuscitation (CPR). METHODS: Fourteen anesthetized pigs underwent CPR after induced ventricular fibrillation. During CPR and return of spontaneous circulation, 2% hydrogen gas was administered to seven pigs (hydrogen group) and seven constituted a control group. Biochemistry and sublingual microcirculation were assessed at baseline, during CPR, at the 15th, 30th, 60th, 120th minute. RESULTS: All seven subjects from the hydrogen group and six subjects in the control group were successfully resuscitated after 6\u201310 minutes. At baseline, there were no statistically significant differences in examined variables. After the CPR, blood pH, base excess, and lactate showed significantly smaller deterioration in the hydrogen group than in the control group. By contrast, plasma syndecan-1 and the measured variables obtained via sublingual microcirculation did not change after the CPR; and were virtually identical between the two groups. CONCLUSION: In pigs, hydrogen gas inhalation during CPR and post-resuscitation care was associated with less pronounced metabolic acidosis compared to controls. However, we could not find evidence of injury to the endothelium or glycocalyx in any studied groups.<\/jats:p>","DOI":"10.3233\/ch-231768","type":"journal-article","created":{"date-parts":[[2023,9,5]],"date-time":"2023-09-05T11:44:51Z","timestamp":1693914291000},"page":"135-146","source":"Crossref","is-referenced-by-count":8,"title":["Protection of the endothelium and endothelial glycocalyx by hydrogen against ischaemia-reperfusion injury in a porcine model of cardiac arrest"],"prefix":"10.1177","volume":"85","author":[{"given":"David","family":"Astapenko","sequence":"first","affiliation":[{"name":"Department of Anesthesiology, Resuscitation, and Intensive Care Medicine, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic"},{"name":"Faculty of Medicine in Hradec Kralove, Charles University, Prague Czech Republic"},{"name":"Faculty of Health Studies, Technical University in Liberec, Liberec, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Radomir","family":"Hyspler","sequence":"additional","affiliation":[{"name":"Faculty of Medicine in Hradec Kralove, Charles University, Prague Czech Republic"},{"name":"Department of Clinical Biochemistry and Diagnostics, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alena","family":"Ticha","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry and Diagnostics, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adela","family":"Tomasova","sequence":"additional","affiliation":[{"name":"Faculty of Medicine in Hradec Kralove, Charles University, Prague Czech Republic"},{"name":"Department of Clinical Biochemistry and Diagnostics, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pavel","family":"Navratil","sequence":"additional","affiliation":[{"name":"Faculty of Medicine in Hradec Kralove, Charles University, Prague Czech Republic"},{"name":"Department of Urology, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marek","family":"Zrzavecky","sequence":"additional","affiliation":[{"name":"Faculty of Medicine in Hradec Kralove, Charles University, Prague Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bhavya","family":"Byreddy","sequence":"additional","affiliation":[{"name":"Faculty of Medicine in Hradec Kralove, Charles University, Prague Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Petr","family":"Sedlacek","sequence":"additional","affiliation":[{"name":"Faculty of Medicine in Hradec Kralove, Charles University, Prague Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vera","family":"Radochova","sequence":"additional","affiliation":[{"name":"Faculty of Military Health Sciences, University of Defence, Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roman","family":"Skulec","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, Resuscitation, and Intensive Care Medicine, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic"},{"name":"Faculty of Medicine in Hradec Kralove, Charles University, Prague Czech Republic"},{"name":"Department of Anesthesiology, Perioperative Medicine, and Intensive Care Medicine, University of J.\u00a0E. Purkyne in Usti nad Labem, Masaryk Hospital in Usti nad Labem, Usti nad Labem, Czech Republic"},{"name":"Institute of Postgradual Education in Healthcare, Prague, Czech Republic"},{"name":"Department of Emergency Medicine, Hospital Bory, Bratislava, Slovak Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert G.","family":"Hahn","sequence":"additional","affiliation":[{"name":"Karolinska Institutet at Danderyds Hospital (KIDS), Stockholm, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Lehmann","sequence":"additional","affiliation":[{"name":"Department of Anesthesia, Pain Management and Perioperative Medicine, Dalhousie University, Halifax, NS, Canada"},{"name":"Department of Microbiology and Immunology, Dalhousie University, Halifax, NS, Canada"},{"name":"Department of Pharmacology, Dalhousie University, Halifax, NS, Canada"},{"name":"Department of Physiology and Biophysics, Dalhousie University, Halifax, NS, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manu L.N.G.","family":"Malbrain","sequence":"additional","affiliation":[{"name":"First Department of Anaesthesiology and Intensive Therapy, Medical University of Lublin, Lublin, Poland"},{"name":"Medical Data Management, Medaman, Pas, Geel, Belgium"},{"name":"International Fluid Academy, Lovenjoel, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladimir","family":"Cerny","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, Resuscitation, and Intensive Care Medicine, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic"},{"name":"Faculty of Medicine in Hradec Kralove, Charles University, Prague Czech Republic"},{"name":"Faculty of Health Studies, Technical University in Liberec, Liberec, Czech Republic"},{"name":"Department of Anesthesiology, Perioperative Medicine, and Intensive Care Medicine, University of J.\u00a0E. Purkyne in Usti nad Labem, Masaryk Hospital in Usti nad Labem, Usti nad Labem, Czech Republic"},{"name":"Institute of Postgradual Education in Healthcare, Prague, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","reference":[{"key":"10.3233\/CH-231768_ref1","doi-asserted-by":"crossref","first-page":"688","DOI":"10.1038\/nm1577","article-title":"Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicals","volume":"13","author":"Ohsawa","year":"2007","journal-title":"Nat Med"},{"key":"10.3233\/CH-231768_ref2","doi-asserted-by":"crossref","first-page":"8724","DOI":"10.3390\/ijms22168724","article-title":"Molecular Hydrogen as a Novel Antitumor Agent: Possible Mechanisms Underlying Gene Expression","volume":"22","author":"Hirano","year":"2021","journal-title":"Int J Mol Sci"},{"key":"10.3233\/CH-231768_ref3","doi-asserted-by":"crossref","first-page":"650","DOI":"10.2174\/1381612826666201221150857","article-title":"Hydrogen Gas Therapy: From Preclinical Studies to Clinical Trials","volume":"27","author":"Sano","year":"2021","journal-title":"Curr Pharm Des"},{"key":"10.3233\/CH-231768_ref4","doi-asserted-by":"crossref","first-page":"940","DOI":"10.1253\/circj.CJ-17-0105","article-title":"The Effects of Hydrogen Gas Inhalation on Adverse Left Ventricular Remodeling After Percutaneous Coronary Intervention for ST-Elevated Myocardial Infarction - First Pilot Study in Humans","volume":"81","author":"Katsumata","year":"2017","journal-title":"Circ J"},{"key":"10.3233\/CH-231768_ref5","doi-asserted-by":"crossref","first-page":"2015","DOI":"10.1111\/j.1600-6143.2008.02359.x","article-title":"Hydrogen inhalation ameliorates oxidative stress in transplantation induced intestinal graft injury","volume":"8","author":"Buchholz","year":"2008","journal-title":"Am J Transplant"},{"key":"10.3233\/CH-231768_ref6","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1097\/TA.0000000000001620","article-title":"Hydrogen gas inhalation inhibits progression to the \u201cirreversible\u201d stage of shock after severe hemorrhage in rats","volume":"83","author":"Matsuoka","year":"2017","journal-title":"J Trauma Acute Care Surg"},{"key":"10.3233\/CH-231768_ref7","doi-asserted-by":"crossref","unstructured":"Tamura T , Hayashida K , Sano M , et al. 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