{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T16:58:18Z","timestamp":1789750698004,"version":"4.0.1"},"reference-count":27,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2023,12,7]],"date-time":"2023-12-07T00:00:00Z","timestamp":1701907200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Clinical Hemorheology and Microcirculation"],"published-print":{"date-parts":[[2024,5,3]]},"abstract":"<jats:sec>\n                    <jats:title>BACKGROUND:<\/jats:title>\n                    <jats:p>Endothelial glycocalyx (EG) plays a crucial role in maintaining the plasma proteins within the intravascular space.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>OBJECTIVE:<\/jats:title>\n                    <jats:p>We studied whether exogenous albumin protects the EG in an experimental model of EG enzymatic damage in rats.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>METHODS:<\/jats:title>\n                    <jats:p>Rats were divided into three groups of 10 animals that received (1) Evans blue (2) Evans blue\u200a+\u200ahyaluronidase, or (3) Evans blue\u200a+\u200ahyaluronidase\u200a+\u200a20% human albumin via the tail vein. Spectrophotometric analysis was performed 2\u200ah later to quantify the leakage of Evans blue-labeled albumin into the heart, lungs, brain, kidneys, liver, small intestine, spleen, and skeletal muscle.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS:<\/jats:title>\n                    <jats:p>Administration of hyaluronidase numerically increased the capillary leakage of Evans blue in all examined tissues. Co-administration of albumin decreased the leakage of albumin in all tissues except the heart. In the lungs, the ratio between the absorbance and dry organ weight decreased from 5.3 \u00b1 2.4 to 1.7 \u00b1 0.5 (mean \u00b1 SD) ( P\u200a&lt;\u200a0.002), and in the liver, the absorbance decreased from 2.2 \u00b1 0.7 to 1.5 \u00b1 0.4 ( P\u200a&lt;\u200a0.011).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION:<\/jats:title>\n                    <jats:p>Exogenous albumin decreased the capillary leakage of albumin which was interpreted as a sign of maintained EG integrity.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3233\/ch-232027","type":"journal-article","created":{"date-parts":[[2023,12,8]],"date-time":"2023-12-08T13:40:51Z","timestamp":1702042851000},"page":"509-517","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":6,"title":["Modulation of the capillary leakage by exogenous albumin in a rat model of endothelial glycocalyx damage"],"prefix":"10.1177","volume":"86","author":[{"given":"David","family":"Astapenko","sequence":"first","affiliation":[{"name":"Department of Anesthesiology, Resuscitation, and Intensive Care Medicine, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic"},{"name":"Faculty of Medicine in Hradec Kralove, Charles University, Prague, Czech Republic"},{"name":"Faculty of Health Studies, Technical University in Liberec, Liberec, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marek","family":"Zrzavecky","sequence":"additional","affiliation":[{"name":"Faculty of Medicine in Hradec Kralove, Charles University, Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana","family":"Gorskaja","sequence":"additional","affiliation":[{"name":"Faculty of Medicine in Hradec Kralove, Charles University, Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Radomir","family":"Hyspler","sequence":"additional","affiliation":[{"name":"Faculty of Medicine in Hradec Kralove, Charles University, Prague, Czech Republic"},{"name":"Department of Clinical Biochemistry and Diagnostics, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alena","family":"Ticha","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry and Diagnostics, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vera","family":"Radochova","sequence":"additional","affiliation":[{"name":"Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Lehmann","sequence":"additional","affiliation":[{"name":"Department of Anesthesia, Pain Management and Perioperative Medicine, Dalhousie University, Halifax, NS, Canada"},{"name":"Department of Microbiology and Immunology, Dalhousie University, Halifax, NS, Canada"},{"name":"Department of Pharmacology, Dalhousie University, Halifax, NS, Canada"},{"name":"Department of Physiology and Biophysics, Dalhousie University, Halifax, NS, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manu L.N.G.","family":"Malbrain","sequence":"additional","affiliation":[{"name":"First Department of Anaesthesiology and Intensive Therapy, Medical University of Lublin, Lublin, Poland"},{"name":"Medical Data Management, Medaman, Geel, Belgium"},{"name":"International Fluid Academy, Lovenjoel, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladimir","family":"Cerny","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, Resuscitation, and Intensive Care Medicine, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic"},{"name":"Faculty of Medicine in Hradec Kralove, Charles University, Prague, Czech Republic"},{"name":"Faculty of Health Studies, Technical University in Liberec, Liberec, Czech Republic"},{"name":"Department of Anesthesiology and Resuscitation, University Hospital Kralovske Vinohrady, Prague, Czech Republic"},{"name":"Faculty of Social Sciences and Health Care, Constantine the Philosopher University in Nitra, Nitra, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert G.","family":"Hahn","sequence":"additional","affiliation":[{"name":"Karolinska Institutet at Danderyds Hospital (KIDS), Stockholm, 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