{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T15:21:09Z","timestamp":1778253669151,"version":"3.51.4"},"reference-count":22,"publisher":"SAGE Publications","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["HAB"],"published-print":{"date-parts":[[2020,12,9]]},"abstract":"<jats:p>BACKGROUND AND AIM: Prostate cancer is the leading cause of death in many countries. It is important to diagnose the disease in the early stages. Current methods detect the disease with low specificity. Examining the expression of genes responsible for disease and their epigenetic regulators are good tools in this regard. MATERIAL AND METHODS: In this prospective case-control study, 40 Iranian patients with cancer, 40 Iranian patients with prostate hyperplasia, and 40 control samples were examined. After blood sampling from each individual, RNA extraction and cDNA synthesis, GSTP1, HDAC, DNMT3A, and DNMT3B expressions were measured in three understudy groups using specific primers and Real-Time PCR method. RESULTS: A reverse correlation was identified between loss of GSTP1 expression and overexpression of HDAC, DNMT3A, and DNMT3B (P value &lt; 0.0001) with a beneficial pattern of cancer development with high efficiency. The significant decrease of GSTP1 expression in patients in comparison to the healthy controls and the elevated expression levels of the studied epigenetic regulators in PCA and BPH samples indicate the impact of the regulators on GSTP1 expression activity. CONCLUSION: This study showed that the measurement of combined GSTP1 and its epigenetic regulators\u2019 expression could be used as suitable genetic markers for the detection and separation of healthy individuals from prostatic patient groups in the Iranian population. However, a similar study in a larger population of case and control could help us to distinguish between normal, benign, and malignant conditions.<\/jats:p>","DOI":"10.3233\/hab-200424","type":"journal-article","created":{"date-parts":[[2020,8,18]],"date-time":"2020-08-18T11:51:20Z","timestamp":1597751480000},"page":"327-334","source":"Crossref","is-referenced-by-count":3,"title":["Investigation of GSTP1 and epigenetic regulators expression pattern in a population of Iranian patients with prostate cancer"],"prefix":"10.1177","volume":"28","author":[{"given":"Mahan","family":"Mohammadi","sequence":"first","affiliation":[{"name":"Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shiva","family":"Irani","sequence":"additional","affiliation":[{"name":"Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iman","family":"Salahshourifar","sequence":"additional","affiliation":[{"name":"Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jalil","family":"Hosseini","sequence":"additional","affiliation":[{"name":"Men\u2019s Health and Reproductive Health Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Afshin","family":"Moradi","sequence":"additional","affiliation":[{"name":"Department of Pathology, Shohadaye Tajrish Hospital, Tehran, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Farkhondeh","family":"Pouresmaeili","sequence":"additional","affiliation":[{"name":"Men\u2019s Health and Reproductive Health Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran"},{"name":"Medical Genetics Department, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","reference":[{"key":"10.3233\/HAB-200424_ref1","doi-asserted-by":"crossref","first-page":"300","DOI":"10.4161\/epi.3.6.7273","article-title":"The role of histone deacetylases in prostate cancer","volume":"3","author":"Abbas","year":"2008","journal-title":"Epigenetics"},{"key":"10.3233\/HAB-200424_ref2","doi-asserted-by":"crossref","first-page":"3","DOI":"10.4103\/1477-3163.93000","article-title":"The cellular functions of RASSF1A and its inactivation in prostate cancer","volume":"11","author":"Amin","year":"2012","journal-title":"J Carcinog"},{"key":"10.3233\/HAB-200424_ref3","doi-asserted-by":"crossref","first-page":"394","DOI":"10.3322\/caac.21492","article-title":"Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries","volume":"68","author":"Bray","year":"2018","journal-title":"CA Cancer J Clin"},{"key":"10.3233\/HAB-200424_ref4","doi-asserted-by":"crossref","first-page":"e25634","DOI":"10.1371\/journal.pone.0025634","article-title":"GSTP1 DNA methylation and expression status is indicative of 5-aza-2\u2019-deoxycytidine efficacy in human prostate cancer cells","volume":"6","author":"Chiam","year":"2011","journal-title":"PLoS One"},{"key":"10.3233\/HAB-200424_ref5","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.freeradbiomed.2016.02.028","article-title":"The role of glutathione reductase and related enzymes on cellular redox homoeostasis network","volume":"95","author":"Couto","year":"2016","journal-title":"Free Radical Biology and Medicine"},{"key":"10.3233\/HAB-200424_ref6","doi-asserted-by":"crossref","first-page":"1189","DOI":"10.3892\/or.2012.2192","article-title":"Increased levels of DNA methyltransferases are associated with the tumorigenic capacity of prostate cancer cells","volume":"29","author":"Gravina","year":"2013","journal-title":"Oncol Rep"},{"key":"10.3233\/HAB-200424_ref7","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.prnil.2017.11.003","article-title":"The incidence of prostate cancer in Iran: A systematic review and meta-analysis","volume":"6","author":"Hassanipour","year":"2018","journal-title":"Prostate Int"},{"key":"10.3233\/HAB-200424_ref8","doi-asserted-by":"crossref","first-page":"660","DOI":"10.1016\/j.eururo.2004.06.014","article-title":"Molecular detection of prostate cancer: A role for GSTP1 hypermethylation","volume":"46","author":"Henrique","year":"2004","journal-title":"Eur Urol"},{"key":"10.3233\/HAB-200424_ref9","doi-asserted-by":"crossref","first-page":"1373","DOI":"10.1038\/cdd.2010.80","article-title":"Glutathione transferases as mediators of signaling pathways involved in cell proliferation and cell death","volume":"17","author":"Laborde","year":"2010","journal-title":"Cell Death & Differentiation"},{"key":"10.3233\/HAB-200424_ref10","doi-asserted-by":"crossref","unstructured":"Y. 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