{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T16:30:32Z","timestamp":1777393832958,"version":"3.51.4"},"reference-count":0,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2015,2,19]],"date-time":"2015-02-19T00:00:00Z","timestamp":1424304000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Alzheimer\u2019s Disease"],"published-print":{"date-parts":[[2015,2,19]]},"abstract":"<jats:p>Widely confirmed reports were published on association between hyperhomocysteinemia, B vitamin deficiency, oxidative stress, and amyloid-\u03b2 in Alzheimer's disease (AD). Homocysteine, cysteine, cysteinylglycine and glutathione are metabolically interrelated thiols that may be potential indicators of health status and disease risk; they all participate in the metabolic pathway of homocysteine. Previous data obtained in one of our laboratories showed that B vitamin deficiency induced exacerbation of AD-like features in TgCRND8 AD mice; these effects were counteracted by S-adenosylmethionine (SAM) supplementation, through the modulation of DNA methylation and antioxidant pathways. Since the cellular response to oxidative stress typically involves alteration in thiols content, a rapid and sensitive HPLC method with fluorescence detection was here used to evaluate the effect of SAM and superoxide-dismutase (SOD) supplementation on thiols level in plasma, in TgCRND8 mice. The quantitative data obtained from HPLC analysis of mice plasma samples showed significant decrease of thiols level when the B vitamin deficient diet was supplemented with SAM + SOD and SOD alone, the latter showing the greatest effect. All these considerations point out the measurement of plasma thiols concentration as a powerful tool of relevance for all clinical purposes involving the evaluation of oxidative stress. The coupling of HPLC with fluorimetric detection, here used, provided a strong method sensitivity allowing thiols determination at very low levels.<\/jats:p>","DOI":"10.3233\/jad-142391","type":"journal-article","created":{"date-parts":[[2018,6,4]],"date-time":"2018-06-04T13:53:24Z","timestamp":1528120404000},"page":"1323-1331","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":31,"title":["Plasma Thiols Levels in Alzheimer's Disease Mice under Diet-Induced Hyperhomocysteinemia: Effect of S-Adenosylmethionine and Superoxide-Dismutase Supplementation"],"prefix":"10.1177","volume":"44","author":[{"given":"Silvia","family":"Persichilli","sequence":"first","affiliation":[{"name":"Istituto di Biochimica e Biochimica Clinica, Universit\u00e1 Cattolica del Sacro Cuore, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacopo","family":"Gervasoni","sequence":"additional","affiliation":[{"name":"Istituto di Biochimica e Biochimica Clinica, Universit\u00e1 Cattolica del Sacro Cuore, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandra","family":"Di Napoli","sequence":"additional","affiliation":[{"name":"Department of Surgery \u201cP. Valdoni\u201d, Sapienza University of Rome, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea","family":"Fuso","sequence":"additional","affiliation":[{"name":"Department of Psychology, Section of Neuroscience, Sapienza University of Rome, Rome, Italy"},{"name":"European Center for Brain Research (CERC)\/IRCCS Santa Lucia Foundation, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vincenzina","family":"Nicolia","sequence":"additional","affiliation":[{"name":"Department of Surgery \u201cP. Valdoni\u201d, Sapienza University of Rome, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruno","family":"Giardina","sequence":"additional","affiliation":[{"name":"Istituto di Biochimica e Biochimica Clinica, Universit\u00e1 Cattolica del Sacro Cuore, Rome, Italy"},{"name":"Istituto di Chimica del Riconoscimento Molecolare \u2013 UOS Roma, Consiglio Nazionale delle Ricerche c\/o Istituto di Biochimica e Biochimica Clinica, Universit\u00e1 Cattolica del Sacro Cuore, Rome, Italy"},{"name":"Centro di Ricerca in Biochimica e Nutrizione dello Sport (CriBeNS), Universit\u00e1 Cattolica del Sacro Cuore, Milano, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sigfrido","family":"Scarpa","sequence":"additional","affiliation":[{"name":"Department of Surgery \u201cP. Valdoni\u201d, Sapienza University of Rome, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia","family":"Desiderio","sequence":"additional","affiliation":[{"name":"Istituto di Chimica del Riconoscimento Molecolare \u2013 UOS Roma, Consiglio Nazionale delle Ricerche c\/o Istituto di Biochimica e Biochimica Clinica, Universit\u00e1 Cattolica del Sacro Cuore, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosaria A.","family":"Cavallaro","sequence":"additional","affiliation":[{"name":"Department of Surgery \u201cP. Valdoni\u201d, Sapienza University of Rome, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2015,2,19]]},"container-title":["Journal of Alzheimer\u2019s Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.3233\/JAD-142391","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.3233\/JAD-142391","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T10:01:46Z","timestamp":1777370506000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.3233\/JAD-142391"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,2,19]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2015,2,19]]}},"alternative-id":["10.3233\/JAD-142391"],"URL":"https:\/\/doi.org\/10.3233\/jad-142391","relation":{},"ISSN":["1387-2877","1875-8908"],"issn-type":[{"value":"1387-2877","type":"print"},{"value":"1875-8908","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,2,19]]}}}