{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T02:13:53Z","timestamp":1781662433643,"version":"3.54.5"},"reference-count":31,"publisher":"SAGE Publications","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JAD"],"published-print":{"date-parts":[[2016,1,22]]},"abstract":"<jats:p>Recently we reported that several monoclonal antibodies that recognize linear segments of amyloid-\u03b2 (A\u03b2) also recognize amyloid fibrils, but not monomers of unrelated sequences, indicating that recognition of a linear sequence segment is not a reliable indicator of sequence specificity. We asked whether any of the commonly used commercially available A\u03b2 antibodies also recognize fibrils of unrelated sequence. Here we report that 4G8, which recognizes residues 18\u201323 of the A\u03b2 sequence and is widely believed to be sequence-specific, also recognizes fibrils formed from \u03b1-synuclein and islet amyloid polypeptide (IAPP). The recognition of amyloid fibrils is aggregation-dependent because 4G8 does not recognize \u03b1-synuclein or IAPP monomer. 4G8 also stains fibrillar \u03b1-synuclein aggregates in human multiple system atrophy brain where it colocalizes with anti-\u03b1-synuclein monoclonal antibody LB509 immunoreactivity. We also found that LB509 recognizes A\u03b2 fibrils, but not monomer, indicating that generic epitope-reactive antibodies are also produced in response to \u03b1-synuclein immunization. Taken together, our results indicate that generic fibril conformational epitope specificity may be a pervasive property among monoclonal antibodies raised against amyloid-forming antigens and that the specificity of their immunoreactivity should be rigorously established and otherwise interpreted with caution.<\/jats:p>","DOI":"10.3233\/jad-150696","type":"journal-article","created":{"date-parts":[[2016,1,22]],"date-time":"2016-01-22T09:14:12Z","timestamp":1453454052000},"page":"517-525","source":"Crossref","is-referenced-by-count":29,"title":["The Anti-Amyloid-\u03b2 Monoclonal Antibody 4G8 Recognizes a Generic Sequence-Independent Epitope Associated with \u03b1-Synuclein and Islet Amyloid Polypeptide Amyloid Fibrils"],"prefix":"10.1177","volume":"50","author":[{"given":"Asa","family":"Hatami","sequence":"first","affiliation":[{"name":"Department of Molecular Biology and Biochemistry, University of California, Irvine, CA, 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