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In this study, we assessed Se status in different biofluids from a subgroup of participants in the Australian Imaging, Biomarkers and Lifestyle Flagship Study of Ageing. As Se in humans can either be an active component of selenoproteins or inactive via non-specific incorporation into other proteins, we used both size exclusion chromatography-inductively coupled plasma-mass spectrometry (SEC-ICP-MS) and tandem mass spectrometry to characterize selenoproteins in serum. We observed no differences in total Se concentration in serum or cerebrospinal fluid of AD subjects compared to mildly cognitively impairment patients and healthy controls. However, Se levels in erythrocytes were decreased in AD compared to controls. SEC-ICP-MS analysis revealed a dominant Se-containing fraction. This fraction was subjected to standard protein purification and a bottom-up proteomics approach to confirm that the abundant Se in the fraction was due, in part, to selenoprotein P. The lack of change in the Se level is at odds with our previous observations in a Brazilian population deficient in Se, and we attribute this to the Australian cohort being Se-replete.<\/jats:p>","DOI":"10.3233\/jad-160622","type":"journal-article","created":{"date-parts":[[2017,2,10]],"date-time":"2017-02-10T09:45:37Z","timestamp":1486719937000},"page":"183-193","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":63,"title":["Selenium Levels in Serum, Red Blood Cells, and Cerebrospinal Fluid of Alzheimer\u2019s Disease Patients: A Report from the Australian Imaging, Biomarker &amp; Lifestyle Flagship Study of Ageing (AIBL)"],"prefix":"10.1177","volume":"57","author":[{"given":"B\u00e1rbara R.","family":"Cardoso","sequence":"first","affiliation":[{"name":"The Florey Institute of Neuroscience and Mental Health, The University of Melbourne, Parkville, VIC, Australia"},{"name":"Department of Food and Experimental 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