{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T11:50:59Z","timestamp":1787313059523,"version":"build-2736575974"},"reference-count":34,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2017,9,18]],"date-time":"2017-09-18T00:00:00Z","timestamp":1505692800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Alzheimer\u2019s Disease"],"published-print":{"date-parts":[[2017,9,18]]},"abstract":"<jats:p>The Golgi apparatus (GA) is a highly dynamic organelle involved in the processing and sorting of cellular proteins. In Alzheimer\u2019s disease (AD), it has been shown to decrease in size and become fragmented in neocortical and hippocampal neuronal subpopulations. This fragmentation and decrease in size of the GA in AD has been related to the accumulation of hyperphosphorylated tau. However, the involvement of other pathological factors associated with the course of the disease, such as the extracellular accumulation of amyloid-\u03b2 (A\u03b2) aggregates, cannot be ruled out, since both pathologies are present in AD patients. Here we use the P301S tauopathy mouse model to examine possible alterations of the GA in neurons that overexpress human tau (P301S mutated gene) in neocortical and hippocampal neurons, using double immunofluorescence techniques and confocal microscopy. Quantitative analysis revealed that neurofibrillary tangle (NFT)-bearing neurons had important morphological alterations and reductions in the surface area and volume of the GA compared with NFT-free neurons. Since in this mouse model there are no A\u03b2 aggregates typical of AD, the present findings support the idea that the progressive accumulation of phospho-tau is associated with structural alterations of the GA, and that these changes may occur in the absence of A\u03b2 pathology.<\/jats:p>","DOI":"10.3233\/jad-170332","type":"journal-article","created":{"date-parts":[[2017,9,12]],"date-time":"2017-09-12T11:14:11Z","timestamp":1505214851000},"page":"651-661","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":9,"title":["Phospho-Tau Accumulation and Structural Alterations of the Golgi Apparatus of Cortical Pyramidal Neurons in the P301S Tauopathy Mouse Model"],"prefix":"10.1177","volume":"60","author":[{"given":"Alejandro","family":"Ant\u00f3n-Fern\u00e1ndez","sequence":"first","affiliation":[{"name":"Instituto Cajal, CSIC, Madrid, Spain"},{"name":"Laboratorio Cajal de Circuitos Corticales (CTB), Universidad Polit\u00e9cnica de Madrid, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jes\u00fas","family":"Merch\u00e1n-Rubira","sequence":"additional","affiliation":[{"name":"Centro de Biolog\u00eda Molecular Severo Ochoa (CSIC-UAM), Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jes\u00fas","family":"Avila","sequence":"additional","affiliation":[{"name":"Centro de Biolog\u00eda Molecular Severo Ochoa (CSIC-UAM), Madrid, Spain"},{"name":"CIBERNED, Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Neurodegenerativas, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F\u00e9lix","family":"Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Centro de Biolog\u00eda Molecular Severo Ochoa (CSIC-UAM), Madrid, Spain"},{"name":"CIBERNED, Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Neurodegenerativas, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier","family":"DeFelipe","sequence":"additional","affiliation":[{"name":"Instituto Cajal, CSIC, Madrid, Spain"},{"name":"Laboratorio Cajal de Circuitos Corticales (CTB), Universidad Polit\u00e9cnica de Madrid, Madrid, Spain"},{"name":"CIBERNED, Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Neurodegenerativas, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alberto","family":"Mu\u00f1oz","sequence":"additional","affiliation":[{"name":"Instituto Cajal, CSIC, Madrid, Spain"},{"name":"Laboratorio Cajal de Circuitos Corticales (CTB), Universidad Polit\u00e9cnica de Madrid, Madrid, Spain"},{"name":"CIBERNED, Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Neurodegenerativas, Spain"},{"name":"Department of Cell Biology, Complutense University, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2025,2,6]]},"reference":[{"key":"ref001","doi-asserted-by":"publisher","DOI":"10.1016\/j.jns.2006.01.019"},{"key":"ref002","doi-asserted-by":"publisher","DOI":"10.1007\/s11064-007-9302-4"},{"key":"ref003","doi-asserted-by":"publisher","DOI":"10.1101\/SQB.1995.060.01.058"},{"key":"ref004","doi-asserted-by":"publisher","DOI":"10.1038\/nrm877"},{"key":"ref005","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijdevneu.2008.05.006"},{"key":"ref006","doi-asserted-by":"publisher","DOI":"10.3389\/fnana.2015.00157"},{"key":"ref007","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.2.742"},{"key":"ref008","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(02)00720-1"},{"key":"ref009","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111141200"},{"key":"ref010","doi-asserted-by":"publisher","DOI":"10.1002\/cm.20157"},{"key":"ref011","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2010.01.015"},{"key":"ref012","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1208635109"},{"key":"ref013","doi-asserted-by":"publisher","DOI":"10.1016\/j.neurobiolaging.2013.11.022"},{"key":"ref014","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2015.00340"},{"key":"ref015","doi-asserted-by":"publisher","DOI":"10.1002\/bies.201400116"},{"key":"ref016","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)62366-8"},{"key":"ref017","doi-asserted-by":"publisher","DOI":"10.1097\/00005072-199509000-00013"},{"key":"ref018","first-page":"415","volume":"148","author":"Stieber A","year":"1996","journal-title":"Am J Pathol"},{"key":"ref019","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2016.10.005"},{"key":"ref020","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2007.01.010"},{"key":"ref021","doi-asserted-by":"publisher","DOI":"10.1111\/bpa.12407"},{"key":"ref022","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(95)11484-E"},{"key":"ref023","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awt088"},{"key":"ref024","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2010.04.009"},{"key":"ref025","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2010.06855.x"},{"key":"ref026","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1320192111"},{"key":"ref027","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2004.07.003"},{"key":"ref028","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1416690112"},{"key":"ref029","doi-asserted-by":"publisher","DOI":"10.1186\/s13195-016-0227-5"},{"key":"ref030","doi-asserted-by":"publisher","DOI":"10.1023\/B:NEUR.0000021904.61387.95"},{"key":"ref031","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(93)91144-H"},{"key":"ref032","doi-asserted-by":"publisher","DOI":"10.2174\/156720508783954695"},{"key":"ref033","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0001647"},{"key":"ref034","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms2669"}],"container-title":["Journal of Alzheimer\u2019s Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.3233\/JAD-170332","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.3233\/JAD-170332","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.3233\/JAD-170332","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T10:04:48Z","timestamp":1777370688000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.3233\/JAD-170332"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,9,18]]},"references-count":34,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2017,9,18]]}},"alternative-id":["10.3233\/JAD-170332"],"URL":"https:\/\/doi.org\/10.3233\/jad-170332","relation":{},"ISSN":["1387-2877","1875-8908"],"issn-type":[{"value":"1387-2877","type":"print"},{"value":"1875-8908","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,9,18]]}}}