{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T06:11:37Z","timestamp":1790403097447,"version":"4.1.0"},"reference-count":59,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2018,2,28]],"date-time":"2018-02-28T00:00:00Z","timestamp":1519776000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Alzheimer\u2019s Disease"],"published-print":{"date-parts":[[2018,3,27]]},"abstract":"<jats:p>\n                    N-terminally truncated pyroglutamate amyloid-\u03b2 (A\u03b2) peptide starting at position 3 represents a significant fraction of A\u03b2 peptides (pE\n                    <jats:sub>3<\/jats:sub>\n                    -A\u03b2) in amyloid plaques of postmortem brains from patients with Alzheimer\u2019s disease (AD) and older persons with Down syndrome (DS). Studies in transgenic mouse models of AD also showed that pE\n                    <jats:sub>3<\/jats:sub>\n                    -A\u03b2 is a major component of plaques, and mouse monoclonal antibody to pE\n                    <jats:sub>3<\/jats:sub>\n                    -A\u03b2 appears to be a desirable therapeutic agent for AD. Since small peptides do not typically elicit a good immune response in mice, but do so favorably in rabbits, our aims were to generate and partially characterize a rabbit monoclonal antibody (RabmAb) to pE\n                    <jats:sub>3<\/jats:sub>\n                    -A\u03b2. The generated RabmAb was found to be specific for pE\n                    <jats:sub>3<\/jats:sub>\n                    -A\u03b2, since it showed no reactivity with A\u03b2\n                    <jats:sub>16<\/jats:sub>\n                    , A\u03b2\n                    <jats:sub>40<\/jats:sub>\n                    , A\u03b2\n                    <jats:sub>42<\/jats:sub>\n                    , A\u03b2\n                    <jats:sub>3-11<\/jats:sub>\n                    , and pE\n                    <jats:sub>11-17<\/jats:sub>\n                    A\u03b2 peptides in an enzyme linked immunosorbent assay (ELISA). The isotype of the antibody was found to be IgG class. The antibody possesses high affinity to pE\n                    <jats:sub>3<\/jats:sub>\n                    -A\u03b2 with dissociation constant (K\n                    <jats:sub>D<\/jats:sub>\n                    ) for the antibody of 1 nM. The epitope of the antibody lies within the sequence of pE\n                    <jats:sub>3<\/jats:sub>\n                    -FRHD. In dot blotting, the optimal detection of pE\n                    <jats:sub>3<\/jats:sub>\n                    -A\u03b2 was at an antibody concentration of 0.5 \u03bcg\/ml. The threshold of pE\n                    <jats:sub>3<\/jats:sub>\n                    -A\u03b2 detection was 2 fmol. The antibody was sensitive enough to detect 10 pg\/ml of pE\n                    <jats:sub>3<\/jats:sub>\n                    -A\u03b2 in sandwich ELISA. pE\n                    <jats:sub>3<\/jats:sub>\n                    -A\u03b2 was detected in AD and DS brain extracts in ELISA and immunoblotting. Immunohistological studies showed immunolabeling of plaques and blood vessels in brains from patients with AD, and DS showing AD pathology. Thus, the antibody can be widely applied in AD and DS research, and therapeutic applications.\n                  <\/jats:p>","DOI":"10.3233\/jad-170898","type":"journal-article","created":{"date-parts":[[2018,3,2]],"date-time":"2018-03-02T15:51:29Z","timestamp":1520005889000},"page":"1635-1649","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":10,"title":["Generation and Partial Characterization of Rabbit Monoclonal Antibody to Pyroglutamate Amyloid-\u03b2\n                    <sub>3-42<\/sub>\n                    (pE\n                    <sub>3<\/sub>\n                    -A\u03b2)"],"prefix":"10.1177","volume":"62","author":[{"given":"Pankaj D.","family":"Mehta","sequence":"first","affiliation":[{"name":"New York State Institute for Basic Research in Developmental Disabilities, Staten Island, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruce A.","family":"Patrick","sequence":"additional","affiliation":[{"name":"New York State Institute for Basic Research in Developmental Disabilities, Staten Island, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Barshatzky","sequence":"additional","affiliation":[{"name":"New York State Institute for Basic Research in Developmental Disabilities, Staten Island, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sangita P.","family":"Mehta","sequence":"additional","affiliation":[{"name":"New York State Institute for Basic Research in Developmental Disabilities, Staten Island, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janusz","family":"Frackowiak","sequence":"additional","affiliation":[{"name":"New York State Institute for Basic Research in Developmental Disabilities, Staten Island, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bozena","family":"Mazur-Kolecka","sequence":"additional","affiliation":[{"name":"New York State Institute for Basic Research in Developmental Disabilities, Staten Island, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jerzy","family":"Wegiel","sequence":"additional","affiliation":[{"name":"New York State Institute for Basic Research in Developmental Disabilities, Staten Island, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Wisniewski","sequence":"additional","affiliation":[{"name":"Center for Cognitive Neurology, New York University School of Medicine, New York, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David L.","family":"Miller","sequence":"additional","affiliation":[{"name":"New York State Institute for Basic Research in Developmental Disabilities, Staten Island, NY, 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