{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T13:01:59Z","timestamp":1782997319048,"version":"3.54.5"},"reference-count":39,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2018,8,7]],"date-time":"2018-08-07T00:00:00Z","timestamp":1533600000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Alzheimer\u2019s Disease"],"published-print":{"date-parts":[[2018,9,25]]},"abstract":"<jats:p>\n                    Amyloid-\u03b2 (A\u03b2) peptides, A\u03b2\n                    <jats:sub>40<\/jats:sub>\n                    , A\u03b2\n                    <jats:sub>42<\/jats:sub>\n                    , and recently A\u03b2\n                    <jats:sub>25\u00a0-\u00a035<\/jats:sub>\n                    , have been directly implicated in the pathogenesis of Alzheimer\u2019s disease (AD). We have previously shown that all three peptides decrease neuronal viability, but A\u03b2\n                    <jats:sub>40<\/jats:sub>\n                    also promotes synaptic disassembling. In this work, we have studied the effects of these peptides on astrocytes in primary culture and found that the three A\u03b2 peptides were internalized by astrocytes and significantly decreased astrocyte viability, while increasing ROS production. A\u03b2 peptide internalization is temperature-dependent, a fact that supports the idea that A\u03b2 peptides are actively endocytosed by astrocytes. However, inhibiting caveolae formation by methyl-beta-cyclodextrin or by silencing caveolin-1 with RNA interference did not prevent A\u03b2 endocytosis, which suggests that A\u03b2 peptides do not use caveolae to enter astrocytes. Conversely, inhibition of clathrin-coated vesicle formation by chlorpromazine or by silencing clathrin with RNA interference significantly decreased A\u03b2 internalization and partially reverted the decrease of astrocyte viability caused by the presence of A\u03b2. These results suggest that A\u03b2 is endocytosed by clathrin-coated vesicles in astrocytes. A\u03b2-loaded astrocytes, when co-incubated with non-treated astrocytes in separate wells but with the same incubation medium, promoted cell death in non-treated astrocytes; a fact that was associated with the presence of A\u03b2 inside previously unloaded astrocytes. This phenomenon was inhibited by the presence of chlorpromazine in the co-incubation medium. These results suggest that astrocyte may perform A\u03b2 transcytosis, a process that could play a role in the clearance of A\u03b2 peptides from the brain to cerebrospinal fluid.\n                  <\/jats:p>","DOI":"10.3233\/jad-180332","type":"journal-article","created":{"date-parts":[[2018,8,10]],"date-time":"2018-08-10T10:51:16Z","timestamp":1533898276000},"page":"1109-1124","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":34,"title":["Endocytosis and Transcytosis of Amyloid-\u03b2 Peptides by Astrocytes: A Possible Mechanism for Amyloid-\u03b2 Clearance in Alzheimer\u2019s Disease"],"prefix":"10.1177","volume":"65","author":[{"given":"Marta","family":"Dom\u00ednguez-Prieto","sequence":"first","affiliation":[{"name":"Instituto de Neurociencias de Castilla y Le\u00f3n, Universidad de Salamanca, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Velasco","sequence":"additional","affiliation":[{"name":"Instituto de Neurociencias de Castilla y Le\u00f3n, Universidad de Salamanca, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arantxa","family":"Tabernero","sequence":"additional","affiliation":[{"name":"Instituto de Neurociencias de Castilla y Le\u00f3n, Universidad de Salamanca, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 M.","family":"Medina","sequence":"additional","affiliation":[{"name":"Instituto de Neurociencias de Castilla y Le\u00f3n, Universidad de Salamanca, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2018,8,7]]},"reference":[{"key":"ref001","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a006262"},{"key":"ref002","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a006270"},{"key":"ref003","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.5b03891"},{"key":"ref004","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(01)00155-5"},{"key":"ref005","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.10391"},{"key":"ref006","doi-asserted-by":"publisher","DOI":"10.3233\/JAD-160346"},{"key":"ref007","doi-asserted-by":"publisher","DOI":"10.1111\/nan.12338"},{"key":"ref008","doi-asserted-by":"publisher","DOI":"10.1002\/ca.980080612"},{"key":"ref009","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(96)12474-5"},{"key":"ref010","first-page":"983","volume":"152","author":"Funato H","year":"1998","journal-title":"Am J 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