{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T17:04:50Z","timestamp":1777395890330,"version":"3.51.4"},"reference-count":12,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2019,10,17]],"date-time":"2019-10-17T00:00:00Z","timestamp":1571270400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Alzheimer\u2019s Disease"],"published-print":{"date-parts":[[2019,11,26]]},"abstract":"<jats:p>\u00a0Amyloid-\u03b2 (A\u03b2) solution injections into an aqueous mobile phase moving through narrow bore stainless-steel capillary tubing results in adsorption of at least 99% A\u03b2 within the tubing or injection valve. However, if flow is stopped for a period of 5\u201310 minutes, then started, wall desorption yields A\u03b2-containing molecules in the new effluent. The amount of desorbed A\u03b2-containing effluent depends on flow rate, period of flow cessation, and number of successive A\u03b2 injections into the same tube without cleaning between injections. Unexpected multiple chromatographic peaks in these experiments seem to imply \u201cseparation\u201d of released, previously adsorbed A\u03b2-containing products in the empty capillary tubing. These preliminary experiments raise questions about possible errors in Alzheimer\u2019s disease (AD) spinal tap analyses, which use stainless-steel needles of approximately the same inner diameter and encounter similar flow rates as those in our capillary experiments. Microliter syringes and HPLC connectors also contain stainless-steel tubing that have similar inner diameter dimensions and similar flow rates. The capillary system involved in these experiments has previously been proposed as a model system for studying the effects of shear on A\u03b2 within the brain because it offers a research environment that provides highly restrictive flow through very small dimension channels. A suggestion is made for the use of this system in exploratory anti-amyloid drug studies in which both the drug and A\u03b2 are injected in the same solution so that both drug and A\u03b2 are subjected to the same shear environment. Reduction in adsorbed A\u03b2 is suggested as an indicator of effective anti-A\u03b2 drugs.<\/jats:p>","DOI":"10.3233\/jad-190522","type":"journal-article","created":{"date-parts":[[2019,10,18]],"date-time":"2019-10-18T11:01:10Z","timestamp":1571396470000},"page":"751-760","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["Preliminary Capillary Flow Experiments with Amyloid-\u03b2, Possible Needle and Capillary A\u03b2 Adsorption, and a Proposal for Drug Evaluation Under Shear Conditions"],"prefix":"10.1177","volume":"72","author":[{"given":"Conrad N.","family":"Trumbore","sequence":"first","affiliation":[{"name":"Department of Chemistry and Biochemistry, University of Delaware, Newark, DE, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennie","family":"Paik","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Massachusetts, Amhurst, MA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Fay","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Massachusetts, Amhurst, MA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard W.","family":"Vachet","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Massachusetts, Amhurst, MA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2019,10,17]]},"reference":[{"key":"ref001","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(00)91537-1"},{"key":"ref002","doi-asserted-by":"publisher","DOI":"10.3233\/JAD-160027"},{"key":"ref003","doi-asserted-by":"publisher","DOI":"10.3233\/JAD-170259"},{"key":"ref004","doi-asserted-by":"publisher","DOI":"10.3233\/JAD-170259"},{"key":"ref005","doi-asserted-by":"publisher","DOI":"10.3233\/JAD-171080"},{"key":"ref006","doi-asserted-by":"publisher","DOI":"10.1016\/bs.pmbts.2019.05.008"},{"key":"ref007","doi-asserted-by":"publisher","DOI":"10.1002\/aic.690351218"},{"key":"ref008","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(01)96224-7"},{"key":"ref009","doi-asserted-by":"publisher","DOI":"10.1093\/protein\/gzp059"},{"key":"ref010","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1218402110"},{"key":"ref011","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-60761-744-0_2"},{"key":"ref012","doi-asserted-by":"publisher","DOI":"10.3389\/fnmol.2017.00098"}],"container-title":["Journal of Alzheimer\u2019s Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.3233\/JAD-190522","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.3233\/JAD-190522","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.3233\/JAD-190522","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T10:07:00Z","timestamp":1777370820000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.3233\/JAD-190522"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,10,17]]},"references-count":12,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2019,11,26]]}},"alternative-id":["10.3233\/JAD-190522"],"URL":"https:\/\/doi.org\/10.3233\/jad-190522","relation":{},"ISSN":["1387-2877","1875-8908"],"issn-type":[{"value":"1387-2877","type":"print"},{"value":"1875-8908","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,10,17]]}}}