{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T18:57:07Z","timestamp":1788289027780,"version":"build-2803163510"},"reference-count":54,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2019,11,18]],"date-time":"2019-11-18T00:00:00Z","timestamp":1574035200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Alzheimer\u2019s Disease"],"published-print":{"date-parts":[[2020,1,7]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>\n                      Because dementia is an emerging problem in the world, biochemical markers of cerebrospinal fluid (CSF) and radio-isotopic analyses are helpful for diagnosing Alzheimer\u2019s disease (AD). Although blood sample is more feasible and plausible than CSF or radiological biomarkers for screening potential AD, measurements of serum amyloid- \u03b2 (A\u03b2), plasma tau, and serum antibodies for A\u03b2\n                      <jats:sub>1\u00a0-\u00a042<\/jats:sub>\n                      are not yet well established.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>We aimed to identify a new serum biomarker to detect mild cognitive impairment (MCI) and AD in comparison to cognitively healthy control by a new peptidome technology.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>\n                      With only 1.5 \u03bcl of serum, we examined a new target plate \u201cBLOTCHIP\n                      <jats:sup>\u00ae<\/jats:sup>\n                      \u201d plus a matrix-assisted laser desorption\/ionization time-of-flight mass spectrometry (MALDI-TOF\/MS) to discriminate control ( n\u200a=\u200a100), MCI ( n\u200a=\u200a60), and AD ( n\u200a=\u200a99). In some subjects, cognitive Mini-Mental State Examination (MMSE) were compared to positron emission tomography (PET) with Pittsburgh compound B (PiB) and the serum probability of dementia (SPD). The mother proteins of candidate serum peptides were examined in autopsied AD brains.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      Apart from A\u03b2 or tau, the present study discovered a new diagnostic 4-peptides-set biomarker for discriminating control, MCI, and AD with 87% of sensitivity and 65% of specificity between control and AD (\n                      <jats:sup>***<\/jats:sup>\n                      p\u200a&lt;\u200a0.001). MMSE score was well correlated to brain A\u03b2 deposition and to SPD of AD. The mother proteins of the four peptides were upregulated for coagulation, complement, and plasticity (three proteins), and was downregulated for anti-inflammation (one protein) in AD brains.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>The present serum biomarker set provides a new, rapid, non-invasive, highly quantitative and low-cost clinical application for dementia screening, and also suggests an alternative pathomechanism of AD for neuroinflammation and neurovascular unit damage.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3233\/jad-191016","type":"journal-article","created":{"date-parts":[[2019,11,19]],"date-time":"2019-11-19T12:00:33Z","timestamp":1574164833000},"page":"217-227","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":21,"title":["A New Serum Biomarker Set to Detect Mild Cognitive Impairment and Alzheimer\u2019s Disease by Peptidome Technology"],"prefix":"10.1177","volume":"73","author":[{"given":"Koji","family":"Abe","sequence":"first","affiliation":[{"name":"Department of Neurology, Okayama University, Okayama, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingwei","family":"Shang","sequence":"additional","affiliation":[{"name":"Department of Neurology, Okayama University, Okayama, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaowen","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Neurology, Okayama University, Okayama, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toru","family":"Yamashita","sequence":"additional","affiliation":[{"name":"Department of Neurology, Okayama University, Okayama, 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