{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T17:11:11Z","timestamp":1777396271022,"version":"3.51.4"},"reference-count":62,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2020,3,11]],"date-time":"2020-03-11T00:00:00Z","timestamp":1583884800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Alzheimer\u2019s Disease"],"published-print":{"date-parts":[[2020,4,21]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Collection of cerebrospinal fluid (CSF) for measurement of amyloid-\u03b2 (A\u03b2) species is a gold standard in Alzheimer\u2019s disease (AD) diagnosis, but has risks. Thus, establishing a low-risk blood A\u03b2 test with high AD sensitivity and specificity is of outmost interest.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>We evaluated the ability of a commercially available plasma A\u03b2 assay to distinguish AD patients from biomarker-healthy controls.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method:<\/jats:title>\n                    <jats:p>\n                      In a case-control design, we examined plasma samples from 44 AD patients (A\u200a+\u200aN+) and 49 controls (A\u2013N\u2013) from a memory clinic. AD was diagnosed using a combination of neuropsychological examination, CSF biomarker analysis and brain imaging. Total A\u03b2\n                      <jats:sub>40<\/jats:sub>\n                      and total A\u03b2\n                      <jats:sub>42<\/jats:sub>\n                      in plasma were measured through enzyme-linked immunosorbent assay (ELISA) technology using ABtest40 and ABtest42 test kits (Araclon Biotech Ltd.). Receiver operating characteristic (ROC) analyses with outcome AD were performed, and sensitivity and specificity were calculated.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      Plasma A\u03b2\n                      <jats:sub>42\/40<\/jats:sub>\n                      was weakly positively correlated with CSF A\u03b2\n                      <jats:sub>42\/40<\/jats:sub>\n                      (Spearman\u2019s rho 0.22; p\u200a=\u200a0.037). Plasma A\u03b2\n                      <jats:sub>42\/40<\/jats:sub>\n                      alone was not able to statistically significantly distinguish between AD patients and controls (AUC 0.58; 95% CI 0.46, 0.70). At a cut-point of 0.076 maximizing sensitivity and specificity, plasma A\u03b2\n                      <jats:sub>42\/40<\/jats:sub>\n                      had a sensitivity of 61.2% and a specificity of 63.6%.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>In this sample, the high-throughput blood A\u03b2 assay was not able to distinguish well between AD patients and controls. Whether or not the assay may be useful in large-scale epidemiological settings remains to be seen.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3233\/jad-200046","type":"journal-article","created":{"date-parts":[[2020,3,13]],"date-time":"2020-03-13T13:05:50Z","timestamp":1584104750000},"page":"1285-1294","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":32,"title":["Plasma Amyloid Concentration in Alzheimer\u2019s Disease: Performance of a High-Throughput Amyloid Assay in Distinguishing Alzheimer\u2019s Disease Cases from Controls"],"prefix":"10.1177","volume":"74","author":[{"given":"Insa","family":"Feinkohl","sequence":"first","affiliation":[{"name":"Max-Delbrueck-Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany"},{"name":"Berlin Institute of Health (BIH), Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carola G.","family":"Schipke","sequence":"additional","affiliation":[{"name":"Berlin Institute of Health (BIH), Berlin, Germany"},{"name":"Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, corporate member of Freie Universit\u00e4t Berlin, Humboldt-Universit\u00e4t zu Berlin and Berlin Institute of Health (BIH), Experimental & Clinical Research Center (ECRC), Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jochen","family":"Kruppa","sequence":"additional","affiliation":[{"name":"Berlin Institute of Health (BIH), Berlin, Germany"},{"name":"Institut f\u00fcr Biometrie und Klinische Epidemiologie, Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, corporate member of Freie Universit\u00e4t Berlin, Humboldt-Universit\u00e4t zu Berlin and Berlin Institute of Health (BIH), Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Felix","family":"Menne","sequence":"additional","affiliation":[{"name":"Berlin Institute of Health (BIH), Berlin, Germany"},{"name":"Department of Psychiatry and Psychotherapy, Campus Benjamin Franklin, Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin, Humboldt-Universit\u00e4t zu Berlin and Berlin Institute of Health (BIH), Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Georg","family":"Winterer","sequence":"additional","affiliation":[{"name":"Berlin Institute of Health (BIH), Berlin, Germany"},{"name":"Pharmaimage Biomarker Solutions GmbH, Berlin, Germany"},{"name":"Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, corporate member of Freie Universit\u00e4t Berlin, Humboldt-Universit\u00e4t zu Berlin and Berlin Institute of Health (BIH), Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tobias","family":"Pischon","sequence":"additional","affiliation":[{"name":"Max-Delbrueck-Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany"},{"name":"Berlin Institute of Health (BIH), Berlin, Germany"},{"name":"Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, corporate member of Freie Universit\u00e4t Berlin, Humboldt-Universit\u00e4t zu Berlin and Berlin Institute of Health (BIH), Berlin, Germany"},{"name":"MDC\/BIH Biobank, Max-Delbrueck-Center for Molecular Medicine in the Helmholtz Association (MDC), and Berlin Institute of Health (BIH), Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oliver","family":"Peters","sequence":"additional","affiliation":[{"name":"Berlin Institute of Health (BIH), Berlin, Germany"},{"name":"Department of Psychiatry and Psychotherapy, Campus Benjamin Franklin, Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin, Humboldt-Universit\u00e4t zu Berlin and Berlin Institute of Health (BIH), Berlin, 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