{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T17:15:18Z","timestamp":1777396518459,"version":"3.51.4"},"reference-count":52,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2020,6,22]],"date-time":"2020-06-22T00:00:00Z","timestamp":1592784000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Alzheimer\u2019s Disease"],"published-print":{"date-parts":[[2020,8,4]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Coordinated calcium influx upon neuronal depolarization activates pathways that phosphorylate CaMKII, ERKs, and the transcription factor CREB and, therefore, expression of pro-survival and neuroprotective genes. Recent evidence indicates that amyloid-\u03b2 protein precursor (A\u03b2PP) is trafficked to synapses and promotes their formation. At the synapse, A\u03b2PP interacts with synaptic proteins involved in vesicle exocytosis and affects calcium channel function.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>Herein, we examined the role of A\u03b2PP in depolarization-induced calcium-mediated signaling using acute cerebral slices from wild-type C57bl\/6 mice and A\u03b2PP\u2013\/\u2013 C57bl\/6 mice.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>Depolarization of acute cerebral slices from wild-type C57bl\/6 and A\u03b2PP\u2013\/\u2013 C57bl\/6 mice was used to induce synaptic signaling. Protein levels were examined by western blot and calcium dynamics were assessed using primary neuronal cultures.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>In the absence of A\u03b2PP, decreased pCaMKII and pERKs levels were observed. This decrease was sensitive to the inhibition of N- and P\/Q-type Voltage Gated Calcium Channels (N- and P\/Q-VGCCs) by \u03c9-conotoxin GVIA and \u03c9-conotoxin MVIIC, respectively, but not to inhibition of L-type VGCCs by nifedipine. However, the absence of A\u03b2PP did not result in a statistically significant decrease of pCREB, which is a known substrate of pERKs. Finally, using calcium imaging, we found that down regulation of A\u03b2PP in cortical neurons results in a decreased response to depolarization and altered kinetics of calcium response.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>A\u03b2PP regulates synaptic activity-mediated neuronal signaling by affecting N- and P\/Q-VGCCs.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3233\/jad-200290","type":"journal-article","created":{"date-parts":[[2020,6,23]],"date-time":"2020-06-23T11:02:12Z","timestamp":1592910132000},"page":"1121-1133","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":4,"title":["Amyloid-\u03b2 Protein Precursor Regulates Depolarization-Induced Calcium-Mediated Synaptic Signaling in Brain Slices"],"prefix":"10.1177","volume":"76","author":[{"given":"Maria","family":"Chatzistavraki","sequence":"first","affiliation":[{"name":"Department of Biology, Division of Animal and Human Physiology, National and Kapodistrian University of Athens, Panepistimiopolis, Ilisia, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Panagiota","family":"Papazafiri","sequence":"additional","affiliation":[{"name":"Department of Biology, Division of Animal and Human Physiology, National and Kapodistrian University of Athens, Panepistimiopolis, Ilisia, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Spiros","family":"Efthimiopoulos","sequence":"additional","affiliation":[{"name":"Department of Biology, Division of Animal and Human Physiology, National and Kapodistrian University of Athens, Panepistimiopolis, Ilisia, 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