{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T02:04:14Z","timestamp":1789524254430,"version":"build-2803163510"},"reference-count":40,"publisher":"SAGE Publications","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JAD"],"published-print":{"date-parts":[[2021,10,26]]},"abstract":"<jats:p>Background: Abnormal morphology and function of neurons in the prefrontal cortex (PFC) are associated with cognitive deficits in rodent models of Alzheimer\u2019s disease (AD), particularly in cortical layer-5 pyramidal neurons that integrate inputs from different sources and project outputs to cortical or subcortical structures. Pyramidal neurons in layer-5 of the PFC can be classified as two subtypes depending on the inducibility of prominent hyperpolarization-activated cation currents (h-current). However, the differences in the neurophysiological alterations between these two subtypes in rodent models of AD remain poorly understood. Objective: To investigate the neurophysiological alterations between two subtypes of pyramidal neurons in hAPP-J20 mice, a transgenic model for early onset AD. Methods: The synaptic transmission and intrinsic excitability of pyramidal neurons were investigated using whole-cell patch recordings. The morphological complexity of pyramidal neurons was detected by biocytin labelling and subsequent Sholl analysis. Results: We found reduced synaptic transmission and intrinsic excitability of the prominent h-current (PH) cells but not the non-PH cells in hAPP-J20 mice. Furthermore, the function of hyperpolarization-activated cyclic nucleotide-gated (HCN) channels which mediated h-current was disrupted in the PH cells of hAPP-J20 mice. Sholl analysis revealed that PH cells had less dendritic intersections in hAPP-J20 mice comparing to control mice, implying that a lower morphological complexity might contribute to the reduced neuronal activity. Conclusion: These results suggest that the PH cells in the medial PFC may be more vulnerable to degeneration in hAPP-J20 mice and play a sustainable role in frontal dysfunction in AD.<\/jats:p>","DOI":"10.3233\/jad-210585","type":"journal-article","created":{"date-parts":[[2021,9,3]],"date-time":"2021-09-03T11:54:50Z","timestamp":1630670090000},"page":"129-140","source":"Crossref","is-referenced-by-count":17,"title":["Reduced Synaptic Transmission and Intrinsic Excitability of a Subtype of Pyramidal Neurons in the Medial Prefrontal Cortex in a Mouse Model of Alzheimer\u2019s Disease"],"prefix":"10.1177","volume":"84","author":[{"given":"Xiao-Qin","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Pharmacology, School of Medicine, Zhejiang Key Laboratory of Pathophysiology, Ningbo University, Ningbo, Zhejiang, China"},{"name":"Key Laboratory of Addiction Research of Zhejiang Province, Ningbo Kangning Hospital, Ningbo, Zhejiang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Le","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, School of Medicine, Zhejiang Key Laboratory of Pathophysiology, Ningbo University, Ningbo, Zhejiang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Si-Yu","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, School of Medicine, Zhejiang Key Laboratory of Pathophysiology, Ningbo University, Ningbo, Zhejiang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin-Bo","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, School of Medicine, Zhejiang Key Laboratory of Pathophysiology, Ningbo University, Ningbo, Zhejiang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei-Yuan","family":"Dong","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, School of Medicine, Zhejiang Key Laboratory of Pathophysiology, Ningbo University, Ningbo, Zhejiang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bing-Gui","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, NHC and CAMS Key Laboratory of Medical Neurobiology, School of Brain Science and Brain Medicine, Zhejiang University, Hangzhou, Zhejiang Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao-Wei","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, School of Medicine, Zhejiang Key Laboratory of Pathophysiology, Ningbo University, Ningbo, Zhejiang, China"},{"name":"Key Laboratory of Addiction Research of Zhejiang Province, Ningbo Kangning Hospital, Ningbo, Zhejiang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","reference":[{"key":"10.3233\/JAD-210585_ref1","first-page":"1869","article-title":"Functional MR imaging in Alzheimer\u2019s disease during memory encoding","volume":"21","author":"Rombouts","year":"2000","journal-title":"AJNR Am J Neuroradiol"},{"key":"10.3233\/JAD-210585_ref2","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1093\/brain\/awr311","article-title":"Executive deficits are related to the inferior frontal junction in early dementia","volume":"135","author":"Schroeter","year":"2012","journal-title":"Brain"},{"key":"10.3233\/JAD-210585_ref3","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1002\/cne.903010105","article-title":"Quantitative analysis of a vulnerable subset of pyramidal neurons in Alzheimer\u2019s disease: I. 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