{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T16:18:42Z","timestamp":1777393122044,"version":"3.51.4"},"reference-count":34,"publisher":"SAGE Publications","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JAD"],"published-print":{"date-parts":[[2024,6,25]]},"abstract":"<jats:p>Background: With the aging of populations worldwide, Alzheimer\u2019s disease (AD) has become a concern due to its high prevalence and the continued lack of established treatments. Early diagnosis is required as a preventive intervention to modify the disease\u2019s progression. In our previous study, we performed peptidomic analysis of serum samples obtained from AD patients and age-matched healthy subjects to seek peptide biomarker candidates for AD by using BLOTCHIP-MS analysis, and identified four peptides as AD biomarker candidates. Objective: The objective was to validate the serum biomarker peptides to distinguish mild cognitive impairment (MCI) and AD in comparison to cognitively healthy controls using a new peptidome technology, the Dementia Risk Test. Methods: We enrolled 195 subjects with normal cognitive function (NC; n\u200a=\u200a70), MCI (n\u200a=\u200a55), and AD (n\u200a=\u200a70), The concentrations of cognitive impairment marker peptides (Fibrinogen \u03b1 chain (FAC), Fibrinogen \u03b2 chain (FBC), Plasma protease C1 inhibitor (PPC1I), \u03b12-HS-glycoprotein (AHSG)) were quantified by using a selected reaction monitoring assay based on liquid chromatography-MS\/MS. Results: The present study confirmed that three peptides, FAC, FBC, and PPC1I, were significantly upregulated during the onset of AD. This three-peptide set was both highly sensitive in determining AD (sensitivity: 85.7%, specificity: 95.7%, AUC: 0.900) and useful in distinguishing MCI (sensitivity: 61.8%, specificity: 98.6%, AUC: 0.824) from NC. Conclusions: In this validation study, we confirmed the high diagnostic potential of the three peptides identified in our previous study as candidate serum biomarkers for AD. The Dementia Risk Test may be a powerful tool for detecting AD-related pathological changes.<\/jats:p>","DOI":"10.3233\/jad-230915","type":"journal-article","created":{"date-parts":[[2024,6,4]],"date-time":"2024-06-04T13:47:36Z","timestamp":1717508856000},"page":"219-228","source":"Crossref","is-referenced-by-count":0,"title":["A Novel Peptidome Technology for the Diagnosis of Mild Cognitive Impairment and Alzheimer\u2019s Disease by Selected Reaction Monitoring"],"prefix":"10.1177","volume":"100","author":[{"given":"Yusuke","family":"Fukui","sequence":"first","affiliation":[{"name":"Department of Neurology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Kitaku, Okayama, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koh","family":"Tadokoro","sequence":"additional","affiliation":[{"name":"Department of Neurology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Kitaku, Okayama, 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