{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T17:43:12Z","timestamp":1789580592855,"version":"build-2803163510"},"reference-count":30,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2024,9,11]],"date-time":"2024-09-11T00:00:00Z","timestamp":1726012800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Alzheimer\u2019s Disease"],"published-print":{"date-parts":[[2024,9,24]]},"abstract":"<jats:sec specific-use=\"heading-level-1\">\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>\n                      There is no molecular test for Alzheimer\u2019s disease (AD) using self-collected samples, nor is there a definitive molecular test for AD. We demonstrate an accurate and potentially definitive TempO-Seq\n                      <jats:sup>\u00ae<\/jats:sup>\n                      gene expression test for AD using fingerstick blood spotted and dried on filter paper, a sample that can be collected in any doctor\u2019s office or can be self-collected.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec specific-use=\"heading-level-1\">\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>Demonstrate the feasibility of developing an accurate test for the classification of persons with AD from a minimally invasive sample of fingerstick blood spotted on filter paper which can be obtained in any doctor\u2019s office or self-collected to address health disparities.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec specific-use=\"heading-level-1\">\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>Fingerstick blood samples from patients clinically diagnosed with AD, Parkinson\u2019s disease (PD), or asymptomatic controls were spotted onto filter paper in the doctor\u2019s office, dried, and shipped to BioSpyder for testing. Three independent patient cohorts were used for training\/retraining and testing\/retesting AD and PD classification algorithms.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec specific-use=\"heading-level-1\">\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>After initially identifying a 770 gene classification signature, a minimum set of 68 genes was identified providing classification test areas under the ROC curve of 0.9 for classifying patients as having AD, and 0.94 for classifying patients as having PD.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec specific-use=\"heading-level-1\">\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>These data demonstrate the potential to develop a screening and\/or definitive, minimally invasive, molecular diagnostic test for AD and PD using dried fingerstick blood spot samples that are collected in a doctor\u2019s office or clinic, or self-collected, and thus, can address health disparities. Whether the test can classify patients with AD earlier then possible with cognitive testing remains to be determined.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3233\/jad-240174","type":"journal-article","created":{"date-parts":[[2024,9,13]],"date-time":"2024-09-13T11:29:39Z","timestamp":1726226979000},"page":"813-822","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":5,"title":["Molecular Gene Expression Testing to Identify Alzheimer\u2019s Disease with High Accuracy from Fingerstick Blood"],"prefix":"10.1177","volume":"101","author":[{"given":"Bruce","family":"Seligmann","sequence":"first","affiliation":[{"name":"BioSpyder Technologies, Inc., Carlsbad, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salvatore","family":"Camiolo","sequence":"additional","affiliation":[{"name":"BioSpyder Technologies, Inc., Carlsbad, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monica","family":"Hernandez","sequence":"additional","affiliation":[{"name":"BioSpyder Technologies, Inc., Carlsbad, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanne M.","family":"Yeakley","sequence":"additional","affiliation":[{"name":"BioSpyder Technologies, Inc., Carlsbad, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory","family":"Sahagian","sequence":"additional","affiliation":[{"name":"Neurology Center of Southern California, Carlsbad, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joel","family":"McComb","sequence":"additional","affiliation":[{"name":"BioSpyder Technologies, Inc., Carlsbad, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2024,9,11]]},"reference":[{"key":"e_1_3_5_2_2","unstructured":"World Health Organization Dementia - WHO 2022 https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/dementia (2022 accessed 1 September 2022)."},{"key":"e_1_3_5_3_2","unstructured":"GauthierG Rosa-NetoP AoraisJJ et al. 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