{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T14:50:08Z","timestamp":1790520608914,"version":"4.1.0"},"reference-count":52,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2021,4,2]],"date-time":"2021-04-02T00:00:00Z","timestamp":1617321600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Neuromuscular Diseases"],"published-print":{"date-parts":[[2021,7,30]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>\n                      The mdx-C57\/B6 mouse model does not show the clinical signs of Duchenne muscular dystrophy (DMD), although muscles exhibit hallmarks of permanent regeneration and alterations in muscle function. The DMD\n                      <jats:sup>mdx<\/jats:sup>\n                      <jats:sup>4Cv<\/jats:sup>\n                      strain exhibits very few revertant dystrophin positive myofibers, making that model suitable for studies on gene and cell therapies.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>\n                      The study appraises the histological evolution of the Tibialis Anterior muscle of WT and DMD\n                      <jats:sup>mdx<\/jats:sup>\n                      <jats:sup>4Cv<\/jats:sup>\n                      mutant from 1 to 24 months.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>Histological analysis included a series of immunostainings of muscle sections for assessing tissue features (fibrosis, lipid deposition, necrosis) and cellular characteristics (size of myofibers, number and distribution of myonuclei, number of satellite cells, vessels, macrophages).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      None of the investigated cell types (satellite cells, endothelial cells, macrophages) showed variations in their density within the tissue in both WT and DMD\n                      <jats:sup>mdx<\/jats:sup>\n                      <jats:sup>4Cv<\/jats:sup>\n                      muscle. However, analyzing their number per myofiber showed that in DMD\n                      <jats:sup>mdx<\/jats:sup>\n                      <jats:sup>4Cv<\/jats:sup>\n                      , myofiber capillarization was increased from 1 to 6 months as compared with WT muscle, then dropped from 12 months. Macrophage number did not vary in WT muscle and peaked at 6 months in DMD\n                      <jats:sup>mdx<\/jats:sup>\n                      <jats:sup>4Cv<\/jats:sup>\n                      muscle. The number of satellite cells per myofiber did not vary in WT muscle while it remained high in DMD\n                      <jats:sup>mdx<\/jats:sup>\n                      <jats:sup>4Cv<\/jats:sup>\n                      muscle, starting to decrease from 12 months and being significantly lower at 24 months of age. Myofiber size was not different in DMD\n                      <jats:sup>mdx<\/jats:sup>\n                      <jats:sup>4Cv<\/jats:sup>\n                      from WT except at 24 months, when it strongly decreased in DMD\n                      <jats:sup>mdx<\/jats:sup>\n                      <jats:sup>4Cv<\/jats:sup>\n                      muscle. Necrosis and lipid deposition were rare in DMD\n                      <jats:sup>mdx<\/jats:sup>\n                      <jats:sup>4Cv<\/jats:sup>\n                      muscle. Fibrosis did not increase with age in DMD\n                      <jats:sup>mdx<\/jats:sup>\n                      <jats:sup>4Cv<\/jats:sup>\n                      muscle and was higher than in WT at 6 and 12 months of age.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>\n                      As a whole, the results show a strong decrease of the myofiber size at 24 months, and an increased capillarization until 6 months of age in DMD\n                      <jats:sup>mdx<\/jats:sup>\n                      <jats:sup>4Cv<\/jats:sup>\n                      as compared with the WT. Thus, DMD\n                      <jats:sup>mdx<\/jats:sup>\n                      <jats:sup>4Cv<\/jats:sup>\n                      mice poorly recapitulates histological DMD features, and its use should take into account the age of the animals according to the purpose of the investigation.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3233\/jnd-200562","type":"journal-article","created":{"date-parts":[[2021,4,6]],"date-time":"2021-04-06T13:04:57Z","timestamp":1617714297000},"page":"513-524","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":6,"title":["Histological Analysis of Tibialis Anterior Muscle of DMD\n                    <sup>mdx4Cv<\/sup>\n                    Mice from 1 to 24 Months"],"prefix":"10.1177","volume":"8","author":[{"given":"Sabrina","family":"Ben Larbi","sequence":"first","affiliation":[{"name":"Institut NeuroMyoG\u00e8ne, Universit\u00e9 Claude Bernard Lyon 1, CNRS 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