{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T22:24:37Z","timestamp":1791498277333,"version":"4.3.4"},"reference-count":30,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2021,8,2]],"date-time":"2021-08-02T00:00:00Z","timestamp":1627862400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JPD"],"published-print":{"date-parts":[[2021,8,2]]},"abstract":"<jats:p>Background: Reduced activity of lysosomal glucocerebrosidase is found in brain tissue from Parkinson\u2019s disease patients. Glucocerebrosidase is also highly expressed in peripheral blood monocytes where its activity is decreased in Parkinson\u2019s disease patients, even in the absence of GBA mutation. Objective: To measure glucocerebrosidase activity in cryopreserved peripheral blood monocytes from 30 Parkinson\u2019s disease patients and 30 matched controls and identify any clinical correlation with disease severity. Methods: Flow cytometry was used to measure lysosomal glucocerebrosidase activity in total, classical, intermediate, and non-classical monocytes. All participants underwent neurological examination and motor severity was assessed by the Movement Disorders Society Unified Parkinson\u2019s Disease Rating Scale. Results: Glucocerebrosidase activity was significantly reduced in the total and classical monocyte populations from the Parkinson\u2019s disease patients compared to controls. GCase activity in classical monocytes was inversely correlated to motor symptom severity. Conclusion: Significant differences in monocyte glucocerebrosidase activity can be detected in Parkinson\u2019s disease patients using cryopreserved mononuclear cells and monocyte GCase activity correlated with motor features of disease. Being able to use cryopreserved cells will facilitate the larger multi-site trials needed to validate monocyte GCase activity as a Parkinson\u2019s disease biomarker.<\/jats:p>","DOI":"10.3233\/jpd-202508","type":"journal-article","created":{"date-parts":[[2021,4,27]],"date-time":"2021-04-27T16:00:29Z","timestamp":1619539229000},"page":"1157-1165","source":"Crossref","is-referenced-by-count":25,"title":["Glucocerebrosidase Activity is Reduced in Cryopreserved Parkinson\u2019s Disease Patient Monocytes and Inversely Correlates with Motor Severity"],"prefix":"10.1177","volume":"11","author":[{"given":"Laura P.","family":"Hughes","sequence":"first","affiliation":[{"name":"Brain and Mind Centre, Faculty of Medicine and Health, The University of Sydney School of Medical Sciences, Camperdown, NSW, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marilia M.M.","family":"Pereira","sequence":"additional","affiliation":[{"name":"Brain and Mind Centre, Faculty of 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