{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T04:58:12Z","timestamp":1792645092769,"version":"4.5.0"},"reference-count":48,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2020,11,27]],"date-time":"2020-11-27T00:00:00Z","timestamp":1606435200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["NPM"],"published-print":{"date-parts":[[2020,11,27]]},"abstract":"<jats:p>BACKGROUND: Hypothermia with xenon gas has been used to reduce brain injury and disability rate after perinatal hypoxia-ischemia. We evaluated xenon gas therapy effects in an in vitro model with or without hypothermia on cultured human airway epithelial cells (Calu-3). METHODS: Calu-3 monolayers were grown at an air-liquid interface and exposed to one of the following conditions: 1) 21% FiO2 at 37\u00b0C (control); 2) 45% FiO2 and 50% xenon at 37\u00b0C; 3) 21% FiO2 and 50% xenon at 32\u00b0C; 4) 45% FiO2 and 50% xenon at 32\u00b0C for 24 hours. Transepithelial resistance (TER) measurements were performed and apical surface fluids were collected and assayed for total protein, IL-6, and IL-8. Three monolayers were used for immunofluorescence localization of zonula occludens-1 (ZO-1). The data were analyzed by one-way ANOVA. RESULTS: TER decreased at 24 hours in all treatment groups. Xenon with hyperoxia and hypothermia resulted in greatest decrease in TER compared with other groups. Immunofluorescence localization of ZO-1 (XY) showed reduced density of ZO-1 rings and incomplete ring-like staining in the 45% FiO2\u2013 50% xenon group at 32\u00b0C compared with other groups. Secretion of total protein was not different among groups. Secretion of IL-6 in 21% FiO2 with xenon group at 32\u00b0C was less than that of the control group. The secretion of IL-8 in 45% FiO2 with xenon at 32\u00b0C was greater than that of other groups. CONCLUSION: Hyperoxia and hypothermia result in detrimental epithelial cell function and inflammation over 24-hour exposure. Xenon gas did not affect cell function or reduce inflammation.<\/jats:p>","DOI":"10.3233\/npm-190364","type":"journal-article","created":{"date-parts":[[2020,5,22]],"date-time":"2020-05-22T11:40:35Z","timestamp":1590147635000},"page":"469-476","source":"Crossref","is-referenced-by-count":3,"title":["Effects of xenon gas on human airway epithelial cells during hyperoxia and hypothermia"],"prefix":"10.1177","volume":"13","author":[{"given":"Y.","family":"Zhu","sequence":"first","affiliation":[{"name":"Center for Pediatric Lung Research, Nemours\/Alfred I. duPont Hospital for Children, Wilmington, DE"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.J.","family":"Mosko","sequence":"additional","affiliation":[{"name":"Center for Pediatric Lung Research, Nemours\/Alfred I. duPont Hospital for Children, Wilmington, DE"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Chidekel","sequence":"additional","affiliation":[{"name":"Center for Pediatric Lung Research, Nemours\/Alfred I. duPont Hospital for Children, Wilmington, DE"},{"name":"Department of Pediatrics, Nemours\/Alfred I. duPont Hospital for Children, Wilmington, DE"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.R.","family":"Wolfson","sequence":"additional","affiliation":[{"name":"Departments of Physiology and Pediatrics, Department of Thoracic Medicine and Surgery, CENTRe: Collaborative for Environmental and Neonatal Therapeutics Research, Center for Inflammation and Translational Clinical Lung Research, Lewis Katz School of Medicine at Temple University, Philadelphia, PA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.H.","family":"Shaffer","sequence":"additional","affiliation":[{"name":"Center for Pediatric Lung Research, Nemours\/Alfred I. duPont Hospital for Children, Wilmington, DE"},{"name":"Departments of Physiology and Pediatrics, Department of Thoracic Medicine and Surgery, CENTRe: Collaborative for Environmental and 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