{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T09:56:08Z","timestamp":1777888568317,"version":"3.51.4"},"reference-count":55,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2018,2,8]],"date-time":"2018-02-08T00:00:00Z","timestamp":1518048000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Restorative Neurology and Neuroscience"],"published-print":{"date-parts":[[2018,2,8]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Retinal ganglion cells (RGCs) of mammals lose the ability to regenerate injured axons during postnatal maturation, but little is known about the underlying molecular mechanisms.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>It remains of particular importance to understand the mechanisms of axonal regeneration to develop new therapeutic approaches for nerve injuries.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>\n                      Retinas from newborn to adult monkeys ( Callithrix jacchus)\n                      <jats:sup>1<\/jats:sup>\n                      were obtained immediately after death and cultured in vitro. Growths of axons were monitored using microscopy and time-lapse video cinematography. Immunohistochemistry, Western blotting, qRT-PCR, and genomics were performed to characterize molecules associated with axonal regeneration and growth. A genomic screen was performed by using retinal explants versus native and non-regenerative explants obtained from eye cadavers on the day of birth, and hybridizing the mRNA with cross-reacting cDNA on conventional human microarrays. Followed the genomic screen, siRNA experiments were conducted to identify the functional involvement of identified candidates.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>Neuron-specific human ribonucleoprotein N (snRPN) was found to be a potential regulator of impaired axonal regeneration during neuronal maturation in these animals. In particular, up-regulation of snRPN was observed during retinal maturation, coinciding with a decline in regenerative ability. Axon regeneration was reactivated in snRPN-knockout retinal ex vivo explants of adult monkey.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>These results suggest that coordinated snRPN-driven activities within the neuron-specific ribonucleoprotein complex regulate the regenerative ability of RGCs in primates, thereby highlighting a potential new role for snRPN within neurons and the possibility of novel postinjury therapies.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3233\/rnn-170780","type":"journal-article","created":{"date-parts":[[2018,2,9]],"date-time":"2018-02-09T11:51:55Z","timestamp":1518177115000},"page":"31-43","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["snRPN controls the ability of neurons to regenerate axons"],"prefix":"10.1177","volume":"36","author":[{"given":"Sonja","family":"Mertsch","sequence":"first","affiliation":[{"name":"Institute of Experimental Ophthalmology and DFG-Excellence Center, Cells in Motion (CiM, area C.4), School of Medicine, University of M\u00fcnster, M\u00fcnster, Germany"},{"name":"Department of Ophthalmology, Laboratory of Experimental Ophthalmology, University Clinic Duesseldorf, Duesseldorf, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katrin","family":"Schlicht","sequence":"additional","affiliation":[{"name":"Institute of Experimental Ophthalmology and DFG-Excellence Center, Cells in Motion (CiM, area C.4), School of Medicine, University of M\u00fcnster, M\u00fcnster, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harutyun","family":"Melkonyan","sequence":"additional","affiliation":[{"name":"Institute of Experimental Ophthalmology and DFG-Excellence Center, Cells in Motion (CiM, area C.4), School of Medicine, University of M\u00fcnster, M\u00fcnster, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan","family":"Schlatt","sequence":"additional","affiliation":[{"name":"Institute of Regenerative Medicine (CeRA) and DFG-Excellence Center, Cells in Motion (CiM, area A.2), School of Medicine, University of M\u00fcnster, M\u00fcnster, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Solon","family":"Thanos","sequence":"additional","affiliation":[{"name":"Institute of Experimental Ophthalmology and DFG-Excellence Center, Cells in Motion (CiM, area C.4), School of Medicine, University of M\u00fcnster, M\u00fcnster, 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