{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T05:38:44Z","timestamp":1789882724176,"version":"4.0.1"},"reference-count":33,"publisher":"SAGE Publications","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["THC"],"published-print":{"date-parts":[[2022,7,8]]},"abstract":"<jats:p>BACKGROUND: In the past, various efforts have been made to investigate diagnostic tools for periprosthetic-joint-infection (PJI). It is little-known about the diagnostic utility of polymerase-chain-reaction (PCR) in this context, especially concerning the role of multiplex-PCR assays comparing with conventional tissue culture. OBJECTIVE: Evaluation of an automated-multiplex-PCR cartridge system for patients with suspicion of PJI in comparison with conventional microbiological culture and 16S-rDNA-PCR. METHODS: On suspicion of PJI synovial fluid specimen were taken preoperatively or periprosthetic tissue was collected intraoperatively. Microbiological analysis included conventional culture, 16S-rDNA-PCR and automated-multiplex-PCR (Unyvero-i60-ITI\u00ae). The European-Bone-and-Joint-Infection-Society (EBJIS) criteria were used for PJI diagnosis. Positive and negative percent agreement was calculated. Total percentage agreement and Cohen\u2019s kappa coefficient were calculated. Sensitivity, specificity and positive predictive value of conventional culture, 16S-rDNA-PCR and multiplex-PCR were calculated. Ten specimens of proved PJI were used as control group. RESULTS: Fifty specimen were suitable for culture. 14 (28%) were classified as PJI, 36 (72%) were aseptic. Coagulase-negative staphylococci was the most frequent detected pathogen. Concordance-rate between mPCR and culture results was 75.6% with a Cohen\u2019s kappa of 0.28. Concordance-rate between mPCR and 16S-rDNA was 82.9%, Cohen\u2019s kappa was 0.13. Concordance analysis between culture results and 16S-rDNA lead to a concordance-rate of 88.9%. Cohen\u2019s kappa was calculated with 0.6. With regard to the microbiological culture as reference, sensitivity of the mPCR was 0.33 and specificity was 0.91. Sensitivity and specificity of the 16S-rDNA-PCR was 0.55 and 0.97. The positive predictive value was 0.57 for the mPCR and 0.83 for the 16S-rDNA-PCR. CONCLUSIONS: Due to fair agreement between mPCR and conventional microbiological culture, the tested multiplex-PCR could be an additional instrument for the detection of PJI but is not superior over the conventional culture.<\/jats:p>","DOI":"10.3233\/thc-213248","type":"journal-article","created":{"date-parts":[[2022,1,18]],"date-time":"2022-01-18T16:48:08Z","timestamp":1642524488000},"page":"1005-1015","source":"Crossref","is-referenced-by-count":10,"title":["Periprosthetic joint infection: Comparison of automated multiplex-PCR Unyvero i60 ITI cartridge system with bacterial culture and real-time PCR"],"prefix":"10.1177","volume":"30","author":[{"given":"Martin","family":"L\u00fcdemann","sequence":"first","affiliation":[{"name":"Department of Orthopaedic Surgery, University of Wuerzburg, Wuerzburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saskia","family":"Sulastyanto","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery, University of Wuerzburg, Wuerzburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Raab","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery, University of Wuerzburg, Wuerzburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christoph","family":"Schoen","sequence":"additional","affiliation":[{"name":"Institute for Hygiene and Microbiology, University of Wuerzburg, Wuerzburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maximilian","family":"Rudert","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery, University of Wuerzburg, Wuerzburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","reference":[{"key":"10.3233\/THC-213248_ref1","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1007\/s11999-009-0945-0","article-title":"The epidemiology of revision total knee arthroplasty in the United States","volume":"468","author":"Bozic","year":"2010","journal-title":"Clin Orthop Relat Res."},{"key":"10.3233\/THC-213248_ref2","doi-asserted-by":"crossref","first-page":"128","DOI":"10.2106\/JBJS.H.00155","article-title":"The epidemiology of revision total hip arthroplasty in the United States","volume":"91","author":"Bozic","year":"2009","journal-title":"J Bone Joint Surg Am."},{"issue":"3","key":"10.3233\/THC-213248_ref3","first-page":"144","article-title":"Future clinical and economic impact of revision total hip and knee arthroplasty","volume":"89","author":"Kurtz","year":"2007","journal-title":"J Bone Joint Surg Am."},{"issue":"8","key":"10.3233\/THC-213248_ref4","first-page":"1746","article-title":"The impact of infection after total hip arthroplasty on hospital and surgeon resource utilization","volume":"87","author":"Bozic","year":"2005","journal-title":"J Bone Joint Surg Am."},{"issue":"2","key":"10.3233\/THC-213248_ref5","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1128\/JCM.02482-12","article-title":"Sonication versus vortexing of implants for diagnosis of prosthetic joint infection","volume":"51","author":"Portillo","year":"2013","journal-title":"J Clin Microbiol."},{"issue":"3","key":"10.3233\/THC-213248_ref6","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1016\/j.diagmicrobio.2014.08.018","article-title":"Treating periprosthetic joint infections as biofilms: Key diagnosis and management strategies","volume":"81","author":"Tzeng","year":"2015","journal-title":"Diagn Microbiol Infect Dis."},{"issue":"9","key":"10.3233\/THC-213248_ref7","doi-asserted-by":"crossref","first-page":"1113","DOI":"10.1086\/522184","article-title":"Clin. 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