{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T17:00:32Z","timestamp":1780765232656,"version":"3.54.1"},"reference-count":36,"publisher":"SAGE Publications","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["THC"],"published-print":{"date-parts":[[2024,11,8]]},"abstract":"<jats:p>BACKGROUND: Ficus benghalensis has been used by local health care practitioners to treat pain, inflammation, rheumatism, and other health issues. OBJECTIVE: In this study, the crude extract and diverse fractions, along with the isolated compound of F. benghalensis were examined for their roles as muscle relaxants, analgesics, and sedatives. METHODS: The extract and isolated compound 1 were screened for muscle-relaxant, analgesic, and sedative actions. The acetic acid-mediated writhing model was utilized for analgesic assessment, the muscle relaxant potential was quantified through traction and inclined plan tests, and the open field test was applied for sedative effects. RESULTS: The extract\/fractions (25, 50, and 100 mg\/kg) and isolated compounds (2.5, 5, 10, and 20 mg\/kg) were tested at various doses. A profound (p&lt; 0.001) reduce in the acetic acid-mediated writhing model was observed against carpachromene (64.44%), followed by ethyl acetate (60.67%) and methanol (58.42%) fractions. A marked (p&lt; 0.001) muscle relaxant activity was noticed against the isolated compound (71.09%), followed by ethyl acetate (66.98%) and methanol (67.10%) fractions. Regarding the sedative effect, a significant action was noted against the isolated compound (71.09%), followed by ethyl acetate (66.98%) and methanol (67.10%) fractions. Furthermore, the binding modes of the isolated compounds were explored using molecular docking. The molecular docking study revealed that the isolated compound possessed good binding affinity for COX2 and GABA. Our isolated compound may possess inhibitory activity against COX2 and GABA receptors. CONCLUSION: The extract and isolated compounds of Ficus benghalensis can be used as analgesics, muscle relaxants, and sedatives. However, detailed molecular and functional analyses are essential to ascertain their function as muscle relaxants, analgesics, and sedatives.<\/jats:p>","DOI":"10.3233\/thc-231703","type":"journal-article","created":{"date-parts":[[2024,7,16]],"date-time":"2024-07-16T12:38:36Z","timestamp":1721133516000},"page":"4019-4030","source":"Crossref","is-referenced-by-count":2,"title":["In vivo biological screening of extract and bioactive compound from Ficus benghalensis L. and their in silico molecular docking analysis"],"prefix":"10.1177","volume":"32","author":[{"given":"Tareq","family":"Abu-Izneid","sequence":"first","affiliation":[{"name":"Pharmaceutical Sciences Program, College of Pharmacy, Al Ain University, Al Ain, United Arab Emirates"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdur","family":"Rauf","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Swabi, Ambar, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saima","family":"Naz","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology and Microbiology, Bacha Khan University, Charsadda, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Ibrahim","sequence":"additional","affiliation":[{"name":"Pharmaceutical Sciences Program, College of Pharmacy, Al Ain University, Al Ain, United Arab Emirates"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naveed","family":"Muhammad","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Abdul Wali Khan University, Mardan, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdul","family":"Wadood","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Abdul Wali Khan University, Mardan, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amar","family":"Ajmal","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Abdul Wali Khan University, Mardan, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hassan A.","family":"Hemeg","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Taibah University, Al-Medinah, Al-Monawara, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shah","family":"Fahad","sequence":"additional","affiliation":[{"name":"Department of Agronomy, Abdul Wali Khan University, Mardan, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muthu","family":"Thiruvengadam","sequence":"additional","affiliation":[{"name":"Department of Crop Science, College of Sanghuh Life Science, Konkuk University, Seoul, 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childbirth","volume":"36","author":"Veale","year":"1992","journal-title":"J Ethnopharmacol."},{"key":"10.3233\/THC-231703_ref3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1021\/np50019a001","article-title":"Current status of the NCl plant and animal product program","volume":"45","author":"Suffness","year":"1982","journal-title":"J Nat Prod."},{"issue":"6","key":"10.3233\/THC-231703_ref4","first-page":"965","article-title":"Medicinal plants in therapy","volume":"63","author":"Farnsworth","year":"1985","journal-title":"Bull World Health Organ."},{"issue":"1","key":"10.3233\/THC-231703_ref5","first-page":"21","article-title":"Ficus bengalensis Linn. an overview","volume":"6","author":"Patil","year":"2010","journal-title":"Int J Pharma Bio Sci."},{"issue":"2","key":"10.3233\/THC-231703_ref6","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/j.jep.2008.06.025","article-title":"Ficus spp.(fig): Ethnobotany and potential as anticancer and anti-inflammatory agents","volume":"119","author":"Lansky","year":"2008","journal-title":"J Ethnopharmacol."},{"issue":"4","key":"10.3233\/THC-231703_ref7","first-page":"246","article-title":"Phytopharmacological and phytochemical properties of three Ficus species-an overview","volume":"1","author":"Joseph","year":"2010","journal-title":"Int J Pharma Bio Sci."},{"issue":"8","key":"10.3233\/THC-231703_ref8","first-page":"722","article-title":"Hydrocarbons from the Leaves of Ficus hispidaRxob., Ficus benghalensis Linn. & Ficus infectoria Linn","volume":"20B","author":"Bhar","year":"1981","journal-title":"Indian J Chem."},{"issue":"2","key":"10.3233\/THC-231703_ref9","first-page":"1","article-title":"Anti-Rheumatic and Antioxidant activity of extract of Stem bark of Ficus bengalensis","volume":"1","author":"Manocha","year":"2011","journal-title":"Res. J Chem Sci."},{"key":"10.3233\/THC-231703_ref10","first-page":"155","article-title":"Fungal endophytes of aerial roots of Ficus benghalensis","volume":"8","author":"Suryanarayanan","year":"2001","journal-title":"Fungal Divers."},{"key":"10.3233\/THC-231703_ref11","first-page":"2312","article-title":"Indian medicinal plants","volume":"8","author":"Kirtikar","year":"2004","journal-title":"International Book Distributor, Deharadun, India."},{"issue":"4","key":"10.3233\/THC-231703_ref12","doi-asserted-by":"crossref","first-page":"271","DOI":"10.4103\/0253-7613.27024","article-title":"Evaluation of the immunomodulatory activity of the methanol extracts of F. bengalensis roots in rats","volume":"38","author":"Gabhe","journal-title":"Indian. J Pharmacol."},{"key":"10.3233\/THC-231703_ref13","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1007\/BF02873662","article-title":"Hypercholesterolemic effect of water extract of the bark of banyan tree F. bengalensis","volume":"10","author":"Shukla","year":"1995","journal-title":"Indian J Clin Biochem."},{"key":"10.3233\/THC-231703_ref14","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1007\/BF01091723","article-title":"A study on composition and hypolipidemic effect of dietary fiber from some plant foods","volume":"38","author":"Agarwal","year":"1988","journal-title":"Plant Food Hum Nutr."},{"key":"10.3233\/THC-231703_ref15","first-page":"470","article-title":"Effect of various extracts of F. bengalensis bark on clonidine and haloperidol-induced catalepsy in mice","volume":"3","author":"Nirmal","year":"2007","journal-title":"Pharmacology"},{"key":"10.3233\/THC-231703_ref16","first-page":"902","article-title":"Antioxidant effect of two flavonoids from the bark of F. bengalensis Linn in hyperlipidemic rats","volume":"36","author":"Augusti","year":"1998","journal-title":"Indian J Exp Biol."},{"key":"10.3233\/THC-231703_ref17","doi-asserted-by":"crossref","first-page":"287","DOI":"10.4103\/0250-474X.42970","article-title":"Immunological studies on the aerial roots of the Indian banyan","volume":"70","author":"Khan","year":"2008","journal-title":"Indian J Pharm Sci."},{"key":"10.3233\/THC-231703_ref18","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1016\/j.foodchem.2005.04.028","article-title":"Antioxidant activity of some Algerian medicinal plants extracts containing phenolic compounds","volume":"97","author":"Djeridane","year":"2006","journal-title":"Food Chem."},{"key":"10.3233\/THC-231703_ref19","doi-asserted-by":"crossref","first-page":"1530","DOI":"10.1002\/mnfr.200700481","article-title":"Isolation of tyrosinase inhibitors from Artocarpus heterophyllus and use of its extract as antibrowning agent","volume":"52","author":"Zheng","year":"2008","journal-title":"Mol Nutr Food Res."},{"key":"10.3233\/THC-231703_ref20","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1515\/znc-2017-0179","article-title":"Muscle relaxant activities of pistagremic acid isolated from Pistacia integerrima","volume":"73","author":"Rauf","year":"2018","journal-title":"Z f\u00fcr Naturforschung C."},{"issue":"1","key":"10.3233\/THC-231703_ref21","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1007\/s13205-015-0322-5","article-title":"Emerging CAM Ziziphus nummularia with in vivo sedative-hypnotic, antipyretic and analgesic attributes","volume":"6","author":"Rauf","year":"2016","journal-title":"3 Biotech."},{"key":"10.3233\/THC-231703_ref22","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-021-89557-x","article-title":"Molecular docking analysis and evaluation of the antibacterial and antioxidant activities of the constituents of Ocimum cufodontii","volume":"11","author":"Aliye","year":"2021","journal-title":"Sci. Rep."},{"key":"10.3233\/THC-231703_ref24","doi-asserted-by":"crossref","first-page":"e89109","DOI":"10.1371\/journal.pone.0089109","article-title":"In silico identification and evaluation of leads for the simultaneous inhibition of protease and helicase activities of HCV NS3\/4A protease using complex-based pharmacophore mapping and virtual screening","volume":"9","author":"Wadood","year":"2014","journal-title":"PloS One."},{"key":"10.3233\/THC-231703_ref25","doi-asserted-by":"crossref","first-page":"1060076","DOI":"10.3389\/fmolb.2023.1060076","article-title":"Identification of novel inhibitors for SARS-CoV-2 as therapeutic options using machine learning-based virtual screening, molecular docking and MD simulation","volume":"10","author":"Samad","year":"2023","journal-title":"Front Mol Biosci."},{"key":"10.3233\/THC-231703_ref26","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1016\/j.jep.2009.02.042","article-title":"Anticonvulsant effect of Ficus religiosa: Role of serotonergic pathways","volume":"123","author":"Singh","year":"2009","journal-title":"J Ethnopharmacol."},{"key":"10.3233\/THC-231703_ref27","first-page":"1","article-title":"Effect of root-extracts of Ficus benghalensis (Banyan) in memory, anxiety, muscle co-ordination, and seizure in animal models","volume":"16","author":"Panday","year":"2016","journal-title":"BMC Complement Med Ther."},{"key":"10.3233\/THC-231703_ref28","first-page":"126","article-title":"Evaluation of anti-inflammatory and analgesic activity of Tridax procumbens Linn against formalin, acetic acid and CFA induced pain models","volume":"3","author":"Prabhu","year":"2011","journal-title":"Int J Pharm Pharm Sci."},{"key":"10.3233\/THC-231703_ref29","doi-asserted-by":"crossref","first-page":"2911","DOI":"10.1016\/S0024-3205(01)01374-1","article-title":"Involvement of vanilloid receptor VR1 and prostanoids in the acid-induced writhing responses of mice","volume":"69","author":"Ikeda","year":"2001","journal-title":"Life Sci"},{"key":"10.3233\/THC-231703_ref30","doi-asserted-by":"crossref","first-page":"3984","DOI":"10.1007\/s00044-012-0406-2","article-title":"Anti-inflammatory and analgesic activities of a new cucurbitacin isolated from Citrullus colocynthis seeds","volume":"22","author":"Marzouk","year":"2013","journal-title":"Med Chem Res."},{"key":"10.3233\/THC-231703_ref31","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1186\/S12906-020-03030-2","article-title":"In vivo analgesic, anti-inflammatory, and sedative activity and a molecular docking study of dinaphthodiospyrol G isolated from Diospyros lotus","volume":"20","author":"Rauf","year":"2020","journal-title":"BMC Complement Med. 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Don) steenis","volume":"6","author":"Alhumaydhi","year":"2021","journal-title":"ACS Omega."},{"key":"10.3233\/THC-231703_ref33","doi-asserted-by":"crossref","first-page":"253","DOI":"10.2147\/JIR.S278514","article-title":"Overview on the discovery and development of anti-inflammatory drugs: Should the focus be on synthesis or degradation of PGE2","volume":"14","author":"Mahesh","year":"2021","journal-title":"J Inflamm Res."},{"key":"10.3233\/THC-231703_ref34","first-page":"2271","article-title":"Prevalence of knee osteoarthritis in the United States: Arthritis data from the Third National Health and Nutrition Examination Survey 1991\u201394","volume":"33","author":"Dillon","year":"2006","journal-title":"Int J Rheumatol."},{"issue":"5","key":"10.3233\/THC-231703_ref35","first-page":"1","article-title":"Celecoxib: Considerations regarding its potential disease-modifying properties in osteoarthritis","volume":"13","author":"Zweers","year":"2001","journal-title":"Arthritis Res Ther."},{"key":"10.3233\/THC-231703_ref36","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1007\/s00726-011-1193-7","article-title":"GABA is an effective immunomodulatory molecule","volume":"45","author":"Jin","year":"2013","journal-title":"Amino acids."},{"key":"10.3233\/THC-231703_ref37","first-page":"120","article-title":"In silico screening of hispolon and its analogs: Pharmacokinetics and molecular docking studies with cyclooxygenase-2 enzyme","volume":"12","author":"Mohammadi","year":"2022","journal-title":"J Appl Pharm Sci."}],"container-title":["Technology and Health Care"],"original-title":[],"link":[{"URL":"https:\/\/content.iospress.com\/download?id=10.3233\/THC-231703","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T05:59:39Z","timestamp":1777615179000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.medra.org\/servlet\/aliasResolver?alias=iospress&doi=10.3233\/THC-231703"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11,8]]},"references-count":36,"journal-issue":{"issue":"6"},"URL":"https:\/\/doi.org\/10.3233\/thc-231703","relation":{},"ISSN":["0928-7329","1878-7401"],"issn-type":[{"value":"0928-7329","type":"print"},{"value":"1878-7401","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,11,8]]}}}