{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T06:35:47Z","timestamp":1791527747348,"version":"4.3.4"},"reference-count":21,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Technology and Health Care"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:sec>\n                    <jats:title>BACKGROUND:<\/jats:title>\n                    <jats:p>Tuberculosis (TB), primarily caused by Mycobacterium tuberculosis, remains a significant global health concern. Targeted Next-Generation Sequencing (tNGS) has emerged as a rapid and comprehensive diagnostic tool for tuberculosis, offering advantages over traditional methods and serving as an effective alternative for drug susceptibility testing and the detection of drug-resistant tuberculosis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>OBJECTIVE:<\/jats:title>\n                    <jats:p>This study aimed to retrospectively analyze the clinical characteristics of pulmonary tuberculosis patients. After explore the application value of targeted next-generation sequencing technology in this patient population, providing valuable insights for clinical diagnosis and treatment.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>METHODS:<\/jats:title>\n                    <jats:p>In this retrospective study, we analyzed data from 65 patients with laboratory-confirmed tuberculosis admitted to Tianjin Baodi Hospital from November 14, 2020, to February 1, 2023. Patients underwent bronchoalveolar lavage fluid (BALF) testing, including acid-fast staining, culture, and tNGS. Biopsies and histopathological examinations were performed on some patients, along with comprehensive radiological assessments for all.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS:<\/jats:title>\n                    <jats:p>Among the 65 pulmonary tuberculosis patients, targeted next-generation sequencing detected pathogens in bronchoalveolar lavage fluid with a positivity rate of 93.8%, significantly higher than traditional methods such as acid-fast staining, culture, and pathology. Compared to bronchoalveolar lavage fluid smear, targeted next-generation sequencing demonstrated significantly higher diagnostic sensitivity (98.46% vs. 26.15%) and accuracy (98.46% vs. 26.15%).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION:<\/jats:title>\n                    <jats:p>Targeted next-generation sequencing, with its high sensitivity and specificity compared to traditional methods, provides unique advantages in detecting pathogens among these patients, highlighting its importance in disease management.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3233\/thc-240823","type":"journal-article","created":{"date-parts":[[2024,8,2]],"date-time":"2024-08-02T09:43:57Z","timestamp":1722591837000},"page":"215-223","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":9,"title":["Bronchial lavage tNGS in the diagnosis of pulmonary tuberculosis"],"prefix":"10.1177","volume":"33","author":[{"given":"Zhongpeng","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine, Tianjin Baodi Hospital, Baodi Clinical College of Tianjin Medical University, Tianjin, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yucong","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine, Tianjin Medical University, Tianjin, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuxiang","family":"Shan","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine, Tianjin Baodi Hospital, Baodi Clinical College of Tianjin Medical University, Tianjin, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2025,1,1]]},"reference":[{"key":"bibr1-THC-240823","doi-asserted-by":"publisher","DOI":"10.1016\/j.drudis.2024.103955"},{"key":"bibr2-THC-240823","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.775030"},{"key":"bibr3-THC-240823","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00632-20"},{"key":"bibr4-THC-240823","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1002794"},{"key":"bibr5-THC-240823","doi-asserted-by":"publisher","DOI":"10.5588\/pha.22.0041"},{"key":"bibr6-THC-240823","unstructured":"[Consensus of clinical pathways of metagenomic next-generation sequencing test in diagnosis of lower respiratory tract infections in China]. Zhonghua Jie He He Hu XI Za Zhi. 2023; 46(4): 322-335."},{"key":"bibr7-THC-240823","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2018.00205"},{"key":"bibr8-THC-240823","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.02469-2017"},{"key":"bibr9-THC-240823","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-019-03734-5"},{"key":"bibr10-THC-240823","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2023.01.031"},{"key":"bibr11-THC-240823","doi-asserted-by":"publisher","DOI":"10.20892\/j.issn.2095-3941.2018.0055"},{"key":"bibr12-THC-240823","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmyco.2016.09.021"},{"key":"bibr13-THC-240823","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc1215305"},{"key":"bibr14-THC-240823","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2020.08.004"},{"key":"bibr15-THC-240823","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2020.12.063"},{"key":"bibr16-THC-240823","unstructured":"[Expert consensus for the application of metagenomic next generation sequencing in the pathogen diagnosis in clinical moderate and severe infections (first edition)]. Zhonghua Wei Zhong Bing Ji Jiu Yi Xue. 2020; 32(5): 531-536."},{"issue":"15","key":"bibr17-THC-240823","first-page":"1087","volume":"103","author":"Wang CB","year":"2023","journal-title":"Zhonghua Yi Xue Za Zhi."},{"key":"bibr18-THC-240823","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2021.01.029"},{"key":"bibr19-THC-240823","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.00526-22"},{"key":"bibr20-THC-240823","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.02605-22"},{"key":"bibr21-THC-240823","doi-asserted-by":"publisher","DOI":"10.1099\/acmi.0.000740.v3"}],"container-title":["Technology and Health Care"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.3233\/THC-240823","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.3233\/THC-240823","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.3233\/THC-240823","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T05:20:39Z","timestamp":1777612839000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.3233\/THC-240823"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1]]},"references-count":21,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,1]]}},"alternative-id":["10.3233\/THC-240823"],"URL":"https:\/\/doi.org\/10.3233\/thc-240823","relation":{},"ISSN":["0928-7329","1878-7401"],"issn-type":[{"value":"0928-7329","type":"print"},{"value":"1878-7401","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1]]}}}