{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T16:36:52Z","timestamp":1777394212782,"version":"3.51.4"},"reference-count":0,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2013,5,21]],"date-time":"2013-05-21T00:00:00Z","timestamp":1369094400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Alzheimer\u2019s Disease"],"published-print":{"date-parts":[[2013,5,21]]},"abstract":"<jats:p>\n                    The amyloid-\u03b2 protein precursor (A\u03b2PP) binds several proteins determining metabolism, processing, and the physiological fate of the former. Among these is Fe65, a protein with specific functional significance for A\u03b2PP, in particular conferring stability when the latter is dephosphorylated on Thr\n                    <jats:sup>668<\/jats:sup>\n                    . Thus, it follows that phosphatases like protein phosphatase 1 (PP1) are relevant to A\u03b2PP processing. Consequently, the identification of A\u03b2PP binding proteins, which can be modulated directly or indirectly by PP1, take on added relevance in terms of biological significance. Using the yeast tri-hybrid system and co-immunoprecipitation assays, we describe a novel tri-complex comprising A\u03b2PP, Fe65 and PP1. We show that the trimeric complex (A\u03b2PP:Fe65:PP1\u03b3) occurs in COS-7 cells, rat hippocampal and cortical primary neurons, and in adult rat hippocampus and cortex. Using overlay assays, we demonstrate that Fe65 is in fact the bridging protein in the complex formed and thus we simultaneously describe another PP1 binding protein. This is singularly important given that PP1 binding proteins determine and confer subcellular localization, as well as substrate specificity, thus regulating the phosphatase activity and subsequent intracellular events. Additionally, we show that this interaction correlates with A\u03b2PP Thr\n                    <jats:sup>668<\/jats:sup>\n                    phosphorylation state, consistent with the role of protein (de)phosphorylation as a key mechanism in regulating cellular events.\n                  <\/jats:p>","DOI":"10.3233\/jad-130095","type":"journal-article","created":{"date-parts":[[2018,6,4]],"date-time":"2018-06-04T11:37:16Z","timestamp":1528112236000},"page":"761-775","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":30,"title":["Identification of a Novel Complex A\u03b2PP:Fe65:PP1 that Regulates A\u03b2PP Thr\n                    <sup>668<\/sup>\n                    Phosphorylation Levels"],"prefix":"10.1177","volume":"35","author":[{"given":"Sandra","family":"Rebelo","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Neuroci\u00eancias e, Centro de Biologia Celular, Sec\u00e7\u00e3o Aut\u00f3noma de Ci\u00eancias da Sa\u00fade, Universidade de Aveiro, Aveiro, Portugal"}]},{"given":"Sara C.","family":"Domingues","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Neuroci\u00eancias e, Centro de Biologia Celular, Sec\u00e7\u00e3o Aut\u00f3noma de Ci\u00eancias da Sa\u00fade, Universidade de Aveiro, Aveiro, Portugal"}]},{"given":"Mariana","family":"Santos","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Neuroci\u00eancias e, Centro de Biologia Celular, Sec\u00e7\u00e3o Aut\u00f3noma de Ci\u00eancias da Sa\u00fade, Universidade de Aveiro, Aveiro, Portugal"}]},{"given":"Margarida","family":"Fardilha","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Transdu\u00e7\u00e3o de Sinais, Centro de Biologia Celular, Sec\u00e7\u00e3o Aut\u00f3noma de Ci\u00eancias da Sa\u00fade, Universidade de Aveiro, Aveiro, Portugal"}]},{"given":"Sara L. C.","family":"Esteves","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Transdu\u00e7\u00e3o de Sinais, Centro de Biologia Celular, Sec\u00e7\u00e3o Aut\u00f3noma de Ci\u00eancias da Sa\u00fade, Universidade de Aveiro, Aveiro, Portugal"}]},{"given":"Sandra Isabel","family":"Vieira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Neuroci\u00eancias e, Centro de Biologia Celular, Sec\u00e7\u00e3o Aut\u00f3noma de Ci\u00eancias da Sa\u00fade, Universidade de Aveiro, Aveiro, Portugal"}]},{"given":"Ana Paula B.","family":"Vint\u00e9m","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Transdu\u00e7\u00e3o de Sinais, Centro de Biologia Celular, Sec\u00e7\u00e3o Aut\u00f3noma de Ci\u00eancias da Sa\u00fade, Universidade de Aveiro, Aveiro, Portugal"}]},{"given":"Wenjuan","family":"Wu","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Transdu\u00e7\u00e3o de Sinais, Centro de Biologia Celular, Sec\u00e7\u00e3o Aut\u00f3noma de Ci\u00eancias da Sa\u00fade, Universidade de Aveiro, Aveiro, Portugal"}]},{"given":"Edgar F.","family":"da Cruz e Silva","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Transdu\u00e7\u00e3o de Sinais, Centro de Biologia Celular, Sec\u00e7\u00e3o Aut\u00f3noma de Ci\u00eancias da Sa\u00fade, Universidade de Aveiro, Aveiro, Portugal"}]},{"given":"Odete A. B.","family":"da Cruz e Silva","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Neuroci\u00eancias e, Centro de Biologia Celular, Sec\u00e7\u00e3o Aut\u00f3noma de Ci\u00eancias da Sa\u00fade, Universidade de Aveiro, Aveiro, Portugal"}]}],"member":"179","published-online":{"date-parts":[[2013,5,21]]},"container-title":["Journal of Alzheimer\u2019s Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.3233\/JAD-130095","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.3233\/JAD-130095","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T10:02:39Z","timestamp":1777370559000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.3233\/JAD-130095"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,5,21]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2013,5,21]]}},"alternative-id":["10.3233\/JAD-130095"],"URL":"https:\/\/doi.org\/10.3233\/jad-130095","relation":{},"ISSN":["1387-2877","1875-8908"],"issn-type":[{"value":"1387-2877","type":"print"},{"value":"1875-8908","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,5,21]]}}}