{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T18:22:19Z","timestamp":1780597339650,"version":"3.54.1"},"reference-count":56,"publisher":"Tech Science Press","issue":"6","license":[{"start":{"date-parts":[[2024,5,23]],"date-time":"2024-05-23T00:00:00Z","timestamp":1716422400000},"content-version":"vor","delay-in-days":143,"URL":"https:\/\/doi.org\/10.32604\/TSP-CROSSMARKPOLICY"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["OR"],"published-print":{"date-parts":[[2024]]},"DOI":"10.32604\/or.2024.049173","type":"journal-article","created":{"date-parts":[[2024,5,6]],"date-time":"2024-05-06T08:31:32Z","timestamp":1714984292000},"page":"1047-1061","update-policy":"https:\/\/doi.org\/10.32604\/tsp-crossmarkpolicy","source":"Crossref","is-referenced-by-count":2,"title":["5-Fluorouracil dose escalation generated desensitized colorectal cancer cells with reduced expression of protein methyltransferases and no epithelial-to-mesenchymal transition potential"],"prefix":"10.32604","volume":"32","author":[{"given":"KIMBERLY","family":"FENECH","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ISAAC","family":"MICALLEF","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"BYRON","family":"BARON","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"17807","published-online":{"date-parts":[[2024]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"101174","DOI":"10.1016\/j.tranon.2021.101174","article-title":"Global colorectal cancer burden in 2020 and projections to 2040","volume":"14","author":"Xi","year":"2021","journal-title":"Translational Oncology"},{"key":"ref2","unstructured":"World Health Organisation (WHO) (2022). Cancer. https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/cancer (accessed on 04\/12\/2023)."},{"key":"ref3","doi-asserted-by":"crossref","first-page":"100458","DOI":"10.1016\/j.lanepe.2022.100458","article-title":"Overall and stage-specific survival of patients with screen-detected colorectal cancer in European countries: A population-based study in 9 countries","volume":"21","author":"Cardoso","year":"2022","journal-title":"The Lancet Regional Health\u2013Europe"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"471","DOI":"10.3390\/cancers10120471","article-title":"Chemoresistance and the self-maintaining tumor microenvironment","volume":"10","author":"Yeldag","year":"2018","journal-title":"Cancers"},{"key":"ref5","first-page":"141","article-title":"Drug resistance and combating drug resistance in cancer","volume":"2","author":"Wang","year":"2019","journal-title":"Cancer Drug Resistance"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"171","DOI":"10.3390\/cancers9120171","article-title":"Regulation of EMT in colorectal cancer: A culprit in metastasis","volume":"9","author":"Vu","year":"2017","journal-title":"Cancers"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1038\/s41580-018-0080-4","article-title":"New insights into the mechanisms of epithelial-mesenchymal transition and implications for cancer","volume":"20","author":"Dongre","year":"2019","journal-title":"Nature Reviews Molecular Cell Biology"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"428","DOI":"10.3390\/cells9020428","article-title":"Epithelial to mesenchymal transition: A mechanism that fuels cancer radio\/chemoresistance","volume":"9","author":"Dudas","year":"2020","journal-title":"Cells"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1007\/s11684-018-0656-6","article-title":"Epithelial-to-mesenchymal transition in cancer: Complexity and opportunities","volume":"12","author":"Zhang","year":"2018","journal-title":"Frontiers of Medicine"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"100773","DOI":"10.1016\/j.tranon.2020.100773","article-title":"Epithelial-mesenchymal transition in cancer: A historical overview","volume":"13","author":"Ribatti","year":"2020","journal-title":"Translational Oncology"},{"key":"ref11","first-page":"0180383","article-title":"Reverse epithelial-mesenchymal transition contributes to the regain of drug sensitivity in tyrosine kinase inhibitor-resistant non-small cell lung cancer cells","volume":"12","author":"Lee","year":"2017","journal-title":"PLoS One"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"e179","DOI":"10.1038\/cddis.2011.61","article-title":"Transcription factors that mediate epithelial-mesenchymal transition lead to multidrug resistance by upregulating ABC transporters","volume":"2","author":"Saxena","year":"2011","journal-title":"Cell Death & Disease"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1158\/1078-0432.CCR-17-1577","article-title":"Epithelial-to-mesenchymal transition antagonizes response to targeted therapies in lung cancer by suppressing BIM","volume":"24","author":"Song","year":"2018","journal-title":"Clinical Cancer Research"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"151","DOI":"10.5487\/TR.2015.31.2.151","article-title":"Epithelial-mesenchymal transition is associated with acquired resistance to 5-fluorocuracil in HT-29 colon cancer cells","volume":"31","author":"Kim","year":"2015","journal-title":"Toxicological Research"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1111\/cas.12884","article-title":"Dysregulation of protein methyltransferases in human cancer: An emerging target class for anticancer therapy","volume":"107","author":"Hamamoto","year":"2016","journal-title":"Cancer Science"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"7830","DOI":"10.1158\/0008-5472.CAN-10-0833","article-title":"H3K9 histone methyltransferase G9a promotes lung cancer invasion and metastasis by silencing the cell adhesion molecule Ep-CAM","volume":"70","author":"Chen","year":"2010","journal-title":"Cancer Research"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"1469","DOI":"10.1172\/JCI57349","article-title":"G9a interacts with snail and is critical for snail-mediated E-cadherin repression in human breast cancer","volume":"122","author":"Dong","year":"2012","journal-title":"The Journal of Clinical Investigation"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1186\/1476-4598-13-189","article-title":"The H3K9 methyltransferase G9a is a marker of aggressive ovarian cancer that promotes peritoneal metastasis","volume":"13","author":"Hua","year":"2014","journal-title":"Molecular Cancer"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1016\/j.neo.2020.09.004","article-title":"Targeting methyltransferase PRMT5 retards the carcinogenesis and metastasis of HNSCC via epigenetically inhibiting twist1 transcription","volume":"22","author":"Fan","year":"2020","journal-title":"Neoplasia"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"4468","DOI":"10.18632\/aging.202407","article-title":"PRMT5 regulates colorectal cancer cell growth and EMT via EGFR\/Akt\/GSK3\u03b2 signaling cascades","volume":"13","author":"Yan","year":"2021","journal-title":"Aging"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"6143","DOI":"10.1038\/onc.2016.154","article-title":"Tumor suppressor SET9 guides the epigenetic plasticity of breast cancer cells and serves as an early-stage biomarker for predicting metastasis","volume":"35","author":"Montenegro","year":"2016","journal-title":"Oncogene"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.bbrc.2021.07.086","article-title":"Set7\/9 controls proliferation and genotoxic drug resistance of NSCLC cells","volume":"572","author":"Daks","year":"2021","journal-title":"Biochemical and Biophysical Research Communications"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1038\/s41392-023-01528-y","article-title":"Methylation across the central dogma in health and diseases: New therapeutic strategies","volume":"8","author":"Liu","year":"2023","journal-title":"Signal Transduction and Targeted Therapy"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"40","DOI":"10.3389\/fonc.2014.00040","volume":"4","author":"McDermott","year":"2014","journal-title":"Frontiers in Oncology"},{"key":"ref25","first-page":"50","article-title":"Redirection of transfusion waste and by-products for Xeno-free research applications","volume":"5","author":"Zammit","year":"2019","journal-title":"Journal of Clinical and Translational Research"},{"key":"ref26","author":"Fenech","year":"2023","journal-title":"Investigating the role of the protein methyltransferases EHMT2 and PRMT5 on the epithelial-to-mesenchymal transition of colorectal cancer (Master of Science Thesis)"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"265","DOI":"10.2144\/000113083","article-title":"TScratch: A novel and simple software tool for automated analysis of monolayer wound healing assays","volume":"46","author":"Geb\u00e4ck","year":"2009","journal-title":"BioTechniques"},{"key":"ref28","first-page":"54719","article-title":"Automated quantification and analysis of cell counting procedures using ImageJ plugins","volume":"117","author":"O\u2019Brien","year":"2016","journal-title":"Journal of Visualised Experiments"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"4546","DOI":"10.1038\/s41388-018-0272-6","volume":"37","author":"Parekh","year":"2018","journal-title":"Oncogene"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1074\/mcp.M115.053272","article-title":"Proteomic analysis revealed the important role of vimentin in human cervical carcinoma HeLa cells desensitized with gambogic acid","volume":"15","author":"Yue","year":"2016","journal-title":"Molecular & Cellular proteomics"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1186\/1476-4598-5-20","article-title":"Cellular response to 5-fluorouracil (5-FU) in 5-FU-resistant colon cancer cell lines during treatment and recovery","volume":"5","author":"de Angelis","year":"2006","journal-title":"Molecular Cancer"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/j.canlet.2010.10.006","article-title":"Dysregulation of microRNA-34a expression causes drug-resistance to 5-FU in human colon cancer DLD-1 cells","volume":"300","author":"Akao","year":"2011","journal-title":"Cancer Letters"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"175253","DOI":"10.1016\/j.ejphar.2022.175253","article-title":"Long-term resistance to 5-fluorouracil promotes epithelial-mesenchymal transition, apoptosis evasion, autophagy, and reduced proliferation rate in colon cancer cells","volume":"933","author":"Sousa-Squiavinato","year":"2022","journal-title":"European Journal of Pharmacology"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1016\/j.bbrc.2011.11.142","article-title":"Chemoresistance to 5-fluorouracil induces epithelial-mesenchymal transition via up-regulation of snail in MCF7 human breast cancer cells","volume":"417","author":"Zhang","year":"2012","journal-title":"Biochemical and Biophysical Research Communications"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1309\/AJCP8EL5FTZSOBIH","article-title":"Histologic changes associated with neoadjuvant chemotherapy are predictive of nodal metastases in patients with high-risk prostate cancer","volume":"133","author":"O\u2019Brien","year":"2010","journal-title":"American Journal of Clinical Pathology"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"4092","DOI":"10.1002\/cncr.26021","article-title":"Formation of solid tumors by a single multinucleated cancer cell","volume":"117","author":"Weihua","year":"2011","journal-title":"Cancer"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"1442","DOI":"10.1158\/1078-0432.CCR-09-2878","article-title":"Patient-derived first-generation xenografts of non-small cell lung cancers: Promising tools for predicting drug responses for personalized chemotherapy","volume":"16","author":"Xin","year":"2010","journal-title":"Clinical Cancer Research"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"2675","DOI":"10.3892\/ol.2015.3136","article-title":"Acquisition of 5-fluorouracil resistance induces epithelial-mesenchymal transitions through the hedgehog signaling pathway in HCT-8 colon cancer cells","volume":"9","author":"Liu","year":"2015","journal-title":"Oncology Letters"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"14662","DOI":"10.1039\/C8RA10502B","volume":"9","author":"Lou","year":"2019","journal-title":"RSC Advances"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1186\/s13148-019-0758-2","article-title":"Histone methyltransferases EHMT1 and EHMT2 (GLP\/G9A) maintain PARP inhibitor resistance in high-grade serous ovarian carcinoma","volume":"11","author":"Watson","year":"2019","journal-title":"Clinical Epigenetics"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1186\/s40170-022-00299-4","article-title":"Tadalafil increases the antitumor activity of 5-FU through inhibiting PRMT5-mediated glycolysis and cell proliferation in colorectal cancer","volume":"10","author":"Shen","year":"2022","journal-title":"Cancer & Metabolism"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"30804","DOI":"10.1371\/journal.pone.0030804","article-title":"Parallel evolution under chemotherapy pressure in 29 breast cancer cell lines results in dissimilar mechanisms of resistance","volume":"7","author":"Tegze","year":"2012","journal-title":"PLoS One"},{"key":"ref43","first-page":"602","article-title":"SET7 interacts with HDAC6 and suppresses the development of colon cancer through inactivation of HDAC6","volume":"12","author":"Zhang","year":"2020","journal-title":"American Journal of Translational Research"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1186\/s13148-021-01160-z","article-title":"Targeting the methyltransferase SETD8 impairs tumor cell survival and overcomes drug resistance independently of p53 status in multiple myeloma","volume":"13","author":"Herviou","year":"2021","journal-title":"Clinical Epigenetics"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"4490","DOI":"10.1038\/s41598-020-61402-7","volume":"10","author":"Wu","year":"2020","journal-title":"Scientific Reports"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1186\/s13578-023-01054-y","volume":"13","author":"Wang","year":"2023","journal-title":"Cell & Bioscience"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"2585","DOI":"10.2147\/OTT.S186806","article-title":"SMYD2-OE promotes oxaliplatin resistance in colon cancer through MDR1\/P-glycoprotein via MEK\/ERK\/AP1 pathway","volume":"12","author":"Ren","year":"2019","journal-title":"OncoTargets and Therapy"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"8377","DOI":"10.7150\/thno.37628","article-title":"Inhibition of SMYD2 suppresses tumor progression by down-regulating microRNA-125b and attenuates multi-drug resistance in renal cell carcinoma","volume":"9","author":"Yan","year":"2019","journal-title":"Theranostics"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1038\/s41580-019-0147-x","article-title":"The importance of non-histone protein methylation in cancer therapy","volume":"20","author":"Rodr\u00edguez-Paredes","year":"2019","journal-title":"Nature Reviews Molecular Cell Biology"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1177\/1756283X16671287","article-title":"Targeting histone methylation for colorectal cancer","volume":"10","author":"Huang","year":"2016","journal-title":"Therapeutic Advances in Gastroenterology"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"1557","DOI":"10.1021\/acs.biochem.5b01129","article-title":"Protein methyltransferases: A distinct, diverse, and dynamic family of enzymes","volume":"55","author":"Boriack-Sjodin","year":"2016","journal-title":"Biochemistry"},{"key":"ref52","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.abb.2019.02.014","article-title":"PRMT7 as a unique member of the protein arginine methyltransferase family: A review","volume":"665","author":"Jain","year":"2019","journal-title":"Archives of Biochemistry and Biophysics"},{"key":"ref53","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.semcancer.2019.08.007","article-title":"SOX2 in development and cancer biology","volume":"67","author":"Novak","year":"2020","journal-title":"Seminars in Cancer Biology"},{"key":"ref54","doi-asserted-by":"crossref","first-page":"17639","DOI":"10.1038\/s41598-018-36251-0","article-title":"Sox2 is associated with cancer stem-like properties in colorectal cancer","volume":"8","author":"Takeda","year":"2018","journal-title":"Scientific Reports"},{"key":"ref55","doi-asserted-by":"crossref","first-page":"98635","DOI":"10.18632\/oncotarget.21709","article-title":"Sox2 Modulates Motility and Enhances Progression of Colorectal Cancer via the Rho-ROCK Signaling Pathway","volume":"8","author":"Zheng","year":"2017","journal-title":"Oncotarget"},{"key":"ref56","first-page":"vdac095","article-title":"Targeting protein arginine methyltransferase 5 sensitizes glioblastoma to trametinib","volume":"4","author":"Banasavadi-Siddegowda","year":"2022","journal-title":"Neurooncology Advances"}],"container-title":["Oncology Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.techscience.com\/files\/or\/2024\/TSP_OR-32-6\/OncolRes-32-06-49173\/OncolRes-32-49173.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,6]],"date-time":"2025-03-06T09:21:42Z","timestamp":1741252902000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.techscience.com\/or\/v32n6\/56600"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024]]},"references-count":56,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2024]]},"published-print":{"date-parts":[[2024]]}},"URL":"https:\/\/doi.org\/10.32604\/or.2024.049173","relation":{},"ISSN":["1555-3906"],"issn-type":[{"value":"1555-3906","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024]]},"assertion":[{"value":"2023-12-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-03-29","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-05-23","order":2,"name":"published","label":"Published Online","group":{"name":"publication_history","label":"Publication History"}}]}}